Prevention Of Disulfide Bond Reduction During Recombinant Production Of Polypeptides - EP2188302

The patent EP2188302 was granted to Genentech on Nov 1, 2017. The application was originally filed on Jul 8, 2008 under application number EP08781481A. The patent is currently recorded with a legal status of "Revoked".

EP2188302

GENENTECH
Application Number
EP08781481A
Filing Date
Jul 8, 2008
Status
Revoked
Mar 10, 2025
Publication Date
Nov 1, 2017
External Links
Slate, Register, Google Patents

Patent Summary

Patent Family

Patent Family

Patent Oppositions (13)

Patent oppositions filed by competitors challenge the validity of a granted patent. These oppositions are typically based on claims of prior art, lack of novelty, or non-obviousness. They are a key part of the process for determining a patent's strength and enforceability.

CompanyOpposition DateRepresentativeOpposition Status

Get instant alerts for new oppositions and patent status changes

SANDOZJan 22, 2020MAIWALDINTERVENTION ADMISSIBLE
DF MP DORRIES FRANK MOLNIA & POHLMAN PATENTANWALTE RECHTSANWALTE PARG MBBAug 1, 2018EDERADMISSIBLE
HOFFMANN EITLEAug 1, 2018HOFFMANN EITLEADMISSIBLE
MAIWALDAug 1, 2018MAIWALDADMISSIBLE
MATHYS & SQUIREAug 1, 2018GRIFFINADMISSIBLE
MERCK SHARP & DOHMEAug 1, 2018CARPMAELS & RANSFORDADMISSIBLE
OETKEAug 1, 2018-ADMISSIBLE
DR H ULRICH DORRIESAug 1, 2018DORRIESWITHDRAWN
DR H ULRICH DORRIESAug 1, 2018DORRIES, HANS ULRICH , DF-MP DORRIES FRANK-MOLNIA & POHLMANWITHDRAWN
BAYERJul 31, 2018DRIEHAUSADMISSIBLE
GRUNECKER PATENT UND RECHTSANWALTE PARTG MBBJul 30, 2018GRUNECKERADMISSIBLE
GRUNECKER PATENT UND RECHTSANWALTE PARTG MBBJul 30, 2018GRUNECKER PATENTADMISSIBLE
MICHALSKI HUTTERMANN & PARTNER PATENTANWALTE MBBJul 30, 2018-ADMISSIBLE

Patent Citations (42) New

Patent citations refer to prior patents cited during different phases such as opposition or international search.

Citation PhasePublication Number
INTERNATIONAL-SEARCH-REPORTWO9726357
OPPOSITIONEP0363126
OPPOSITIONEP0612846
OPPOSITIONEP1228076
OPPOSITIONEP1241250
OPPOSITIONEP2188302
OPPOSITIONUS2004029229
OPPOSITIONUS2004138424
OPPOSITIONUS2005038231
OPPOSITIONUS2005100984
OPPOSITIONUS2006105389
OPPOSITIONUS2006142549
OPPOSITIONUS2006216790
OPPOSITIONUS4572798
OPPOSITIONUS5808006
OPPOSITIONUS5830761
OPPOSITIONUS5866362
OPPOSITIONUS6566514
OPPOSITIONUS6872563
OPPOSITIONUS7189811
OPPOSITIONUS7723490
OPPOSITIONUS85573406P
OPPOSITIONWO0012537
OPPOSITIONWO0220818
OPPOSITIONWO2004001056
OPPOSITIONWO2005016968
OPPOSITIONWO2006034488
OPPOSITIONWO2006047340
OPPOSITIONWO2006054063
OPPOSITIONWO2006060083
OPPOSITIONWO2006084264
OPPOSITIONWO2008127305
OPPOSITIONWO2009009523
OPPOSITIONWO9310260
OPPOSITIONWO9726357
OPPOSITIONWO9814467
OPPOSITIONWO9821234
OPPOSITIONWO9933988
OPPOSITIONWO9954440
OTHERUS2004138424
OTHERUS2006142549
OTHERWO2008127305

Non-Patent Literature (NPL) Citations (127) New

NPL citations refer to non-patent references such as research papers, articles, or other publications cited during examination or opposition phases.

Citation PhaseReference Text
INTERNATIONAL-SEARCH-REPORT- SMITH M A ET AL, "SPECIFIC CLEAVAGE OF IMMUNOGLOBULIN G BY COPPER IONS", INTERNATIONAL JOURNAL OF PEPTIDE AND PROTEIN RESEARCH, MUNKSGAARD, COPENHAGEN, DK, (19960701), vol. 48, no. 1, ISSN 0367-8377, pages 48 - 55, XP000594105 [A] 1-55,58-63
INTERNATIONAL-SEARCH-REPORT- TEILUM K ET AL, "Disulfide Bond Formation and Folding of Plant Peroxidases Expressed as Inclusion Body Protein inEscherichia coliThioredoxin Reductase Negative Strains", PROTEIN EXPRESSION AND PURIFICATION, ACADEMIC PRESS, SAN DIEGO, CA, (19990201), vol. 15, no. 1, ISSN 1046-5928, pages 77 - 82, XP004441744 [Y] 3,5,7-10,19-37,39-55,61-63 * abstract * * page 77, right-hand column, last sentence of last full paragraph. *
INTERNATIONAL-SEARCH-REPORT- LI LAN ET AL, "Low level formation of potent catalytic IgG fragments mediated by disulfide bond instability", MOLECULAR IMMUNOLOGY, (1996), vol. 33, no. 7-8, ISSN 0161-5890, pages 593 - 600, XP002519389
INTERNATIONAL-SEARCH-REPORT- URIG S ET AL, "On the potential of thioredoxin reductase inhibitors for cancer therapy", SEMINARS IN CANCER BIOLOGY, SAUNDERS SCIENTIFIC PUBLICATIONS, PHILADELPHIA, PA, US, vol. 16, no. 6, ISSN 1044-579X, (20061201), pages 452 - 465, (20061201), XP024908082 [Y] 3,5,7-10,19-37,39-55,61-63 * the whole document *
INTERNATIONAL-SEARCH-REPORT- POWIS G ET AL, "Thioredoxin redox control of cell growth and death and the effects of inhibitors", CHEMICO-BIOLOGICAL INTERACTIONS 19980424 IE, (19980424), vol. 111-112, ISSN 0009-2797, pages 23 - 34, XP002519386 [Y] 3,5,7-10,19-37,39-55,61-63 * the whole document *
INTERNATIONAL-SEARCH-REPORT- NORDBERG J ET AL, "Reactive oxygen species, antioxidants, and the mammalian thioredoxin system.", FREE RADICAL BIOLOGY & MEDICINE 1 DEC 2001, (20011201), vol. 31, no. 11, ISSN 0891-5849, pages 1287 - 1312, XP002519388 [Y] 3,5,7-10,19-37,39-55,61-63 * the whole document *
INTERNATIONAL-SEARCH-REPORT- SALAS-SOLANO OSCAR ET AL, "Optimization and validation of a quantitative capillary electrophoresis sodium dodecyl sulfate method for quality control and stability monitoring of monoclonal antibodies.", ANALYTICAL CHEMISTRY 15 SEP 2006, (20060915), vol. 78, no. 18, ISSN 0003-2700, pages 6583 - 6594, XP002519390
INTERNATIONAL-SEARCH-REPORT- ZHANG WEI ET AL, "Free sulfhydryl in recombinant monoclonal antibodies", BIOTECHNOLOGY PROGRESS, (200205), vol. 18, no. 3, ISSN 8756-7938, pages 509 - 513, XP002500112 [A] 1-55,58-63
INTERNATIONAL-SEARCH-REPORT- CHADERJIAN WENDY B ET AL, "Effect of copper sulfate on performance of a serum-free CHO cell culture process and the level of free thiol in the recombinant antibody expressed", BIOTECHNOLOGY PROGRESS, (200503), vol. 21, no. 2, ISSN 8756-7938, pages 550 - 553, XP002500111 [XD] 1,2,4,6,11-18,38,58-60 * the whole document * [Y] 3,5,7-10,19-37,39-55,61-63
INTERNATIONAL-SEARCH-REPORT- KERBLAT I ET AL, "Importance of thioredoxin in the proteolysis of an immunoglobulin G as antigen by lysosomal Cys-proteases", IMMUNOLOGY, (199905), vol. 97, no. 1, ISSN 0019-2805, pages 62 - 68, XP002519384 [Y] 3,5,7-10,19-37,39-55,61-63 * the whole document *
INTERNATIONAL-SEARCH-REPORT- POWIS G ET AL, "Properties and biological activities of thioredoxins", ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY 2001 US, (2001), vol. 41, ISSN 0362-1642, pages 261 - 295, XP002519387 [Y] 3,5,7-10,19-37,39-55,61-63 * the whole document *
INTERNATIONAL-SEARCH-REPORT- BOBOVNIKOVA Y ET AL, "Characterization of soluble, disulfide bond-stabilized, prokaryotically depressed human thyrotropin receptor ectodomain", ENDOCRINOLOGY, (1997), vol. 138, no. 2, ISSN 0013-7227, pages 588 - 593, XP002519385 [Y] 3,5,7-10,19-37,39-55,61-63 * abstract * * page 588, column R, paragraphs 1-6 *
OPPOSITION- "9.9 Mammals", C HOWE, Gene Cloning and Manipulation, Cambridge University Press, Cambridge University Press, page 185, XP055515279
OPPOSITION- A. HOLMGREN, "Thioredoxin catalyzes the reduction of insulin disulfides by dithiothreitol and dihydrolipoamide.", The Journal of Biological Chemistry, (19890000), vol. 254, no. 9, pages 9627 - 9632, XP055514845
OPPOSITION- AJOUZ et al., "Release of Thioredoxin via the Mechanosensitive Channel MscL during Osmotic Downshock of Escherichia coli Cells", J. Biological Chemistry, (19980000), vol. 273, no. 41, pages 26670 - 26674, XP055514779
OPPOSITION- Anonymous, "1,2-bis(2-Aminophenoxy)ethane-N,N,N'N'-tetraacetic acid", Sigma, (20021200), XP055514973
OPPOSITION- Anonymous, "EDTA and EGTA checlating agents products information", Interchim
OPPOSITION- Anonymous, "Hexokinase from Saccharomyces cerevisiae", Sigma Product Information, (20170310), pages 1 - 2, XP055515441
OPPOSITION- Anonymous, "L-Cystein Product number C 8755", Sigma, (20030100), XP055515385
OPPOSITION- Becker et al., "Thioredoxin reductase as a pathophysiological factor and drug target", Eur. J. Biochem., (20000000), vol. 267, pages 6118 - 6125, XP055515415
OPPOSITION- Berg et al., "20.3 Der Pentosephosphatweg erzeugt NADPH und C5-Kohlenhydrate", Biochemie, Heidelberg Berlin, pages 616 - 617, XP055514951
OPPOSITION- BERGGREN et al., "Thioredoxin and thioredoxin reductase gene expression in human tumors and cell lines, and the effects of serum stimulation and hypoxia.", Anticancer Research, (19960000), vol. 16, pages 3459 - 3466, XP002062159
OPPOSITION- Drugbank, (20071118), Database accession no. DB05829, XP055514766
OPPOSITION- Excerpt from DrugBank showing the HC/LC sequences of Not used as prior art Trastuzumab
OPPOSITION- FUJIKI et al., "Studies on the disulfide bonds in human pituitary follicle­ stimulating hormone.", Biochim Biophys Acta, (19800000), vol. 624, no. 2, XP055515352
OPPOSITION- G. DE LA FUENTE, "Specific Inactivation of Yeast Hexokinase Induced by Xylose in the Presence of a Phosphoryl Donor Substrate", Eur. J. Biochem., (19700000), vol. 16, pages 240 - 243, XP055514910
OPPOSITION- HIGASHI et al., "Conformation of Factor Vila Stabilized by a Labile Disulfide Bond (Cys-310-Cys-329) in the Protease Domain Is Essential for Interaction with Tissue Factor", The Journal of Biological Chemistry, (19970000), vol. 272, no. 41, pages 25724 - 25730, XP055515357
OPPOSITION- JUNN et al., "Vitamin D3 up-regulated protein 1 mediates oxidative stress via suppressing the thioredoxin function", J Immunol, (20000000), vol. 164, pages 6287 - 6295, XP002226293
OPPOSITION- KAJI et al., "Adenosine Triphosphate Activity of Yeast Hexokinase and Its Relation to the Mechanism of the Hexokinase Reaction", The Journal of Biological Chemistry, (19650000), vol. 240, no. 11, pages 4454 - 4462, XP055514955
OPPOSITION- List of approved antibodies as of 2017 Not used as prior art
OPPOSITION- LUNN et al., "Localization of thioredoxin from Escherichia coli in an osmotically sensitive compartment.", J. Biological Chemistry, (19810000), vol. 257, no. 19, pages 11424 - 11430, XP000606609
OPPOSITION- MCDONALD, "Yeast Hexokinase", Method Enzymol., (19550000), pages 269 - 276, XP055515268
OPPOSITION- "Methoden zur Isolierung von Proteinen", Geoffrey Zubay, BIOCHEMIE, McGraw-Hill International (UK) Ltd, (20000000), pages 147 - 148, XP055514932
OPPOSITION- MILHAUSEN et al., "Evidence for an essential lysine in glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides.", Eur. J. Biochem., (19750000), vol. 50, pages 453 - 461, XP055515265
OPPOSITION- R K Finn, "AGITATION-AERATION IN THE LABORATORY AND IN INDUSTRY", Finn Bacteriol Rev., (19540000), vol. 18, pages 254 - 274, XP055515363
OPPOSITION- SAMBROOK et al., "Lysis of Cells", Molecular Cloning A Laboratory Manual, Cold Spring Harbor Laboratory Press, (19890000), pages 18.30 - 18.31, XP055514944
OPPOSITION- "Sealing up animal cell culture", M BUTLER, Animal Cell Culture and Technology, (20040000), page 183, XP055515374
OPPOSITION- VOET, "2. The reaction of glycolysis", Biochemistry, (19950000), page 447,618,786,811, XP055514967
OPPOSITION- HUTCHINSON et al., "Shear stress analysis of mammalian cell suspensions for prediction of industrial centrifugation and its verification", Biotechnology and Bioengineering, (20060000), vol. 95, no. 3, pages 483 - 491, XP055514888
OPPOSITION- SANDBERG et al., "Mapping and Partial Characterization of Proteases Expressed by a CHO Production Cell Line", Biotechnology and Bioengineering, (20060000), vol. 95, no. 5, pages 961 - 971, XP002453336
OPPOSITION- TREXLER-SCHMIDT et al., "Identification and prevention of antibody disulfide bond reduction during cell culture manufacturing", Biotechnol. Bioeng., (20100000), vol. 106, no. 3, pages 452 - 461, XP002694206
OPPOSITION- TREXLER-SCHMIDT et al., "Identification and prevention of antibody disulfide bond reduction during cell culture manufacturing", Biotechnology and Bioengineering, (20100000), vol. 106, no. 3, pages 452 - 461, XP002694206
OPPOSITION- KAO et al., "Mechanism of Antibody Reduction in Cell Culture Production Processes", Biotechnol. Bioeng., (20100000), vol. 107, no. 4, pages 622 - 632, XP002694207
OPPOSITION- KAO et al., "Mechanism of Antibody Reduction in Cell Culture Production Processes", Biotechnology and Bioengineering, (20100000), vol. 107, no. 4, pages 622 - 632, XP002694207
OPPOSITION- BACKER et al., "Large-scale production of monoclonal antibodies in suspension culture.", Biotechnol. Bioeng., (19880000), vol. 32, pages 993 - 1000, XP055315406
OPPOSITION- FISCHER et al., "Isolation, renaturation, and formation of disulfide bonds of eukaryotic proteins expressed in Escherichia coli as inclusion bodies", Biotechnology and Bioengineering, (19930000), vol. 41, pages 3 - 13, XP002017121
OPPOSITION- GROMER et al., "The Thioredoxin System - From Science to Clinic", Medicinal Research Reviews, (20040000), vol. 24, no. 1, pages 40 - 89, XP002694209
OPPOSITION- GROMER S et al., "The thioredoxin system: From science to clinic", Med. Res. Rev., (20040000), vol. 24, no. 1, pages 40 - 89, XP002694209
OPPOSITION- MICHAELS JD et al., "Sparging and agitation-induced injury of cultured animals cells: Do cell-to-bubble interactions in the bulk liquid injure cells?", Biotechnology and Bioengineering, (19960000), vol. 51, pages 399 - 409, XP055514716
OPPOSITION- GORDON G et al., "On the mechanism of interation of steroids with human glucose 6-phosphate dehydrogenase", Archives of Biochemistry and Biophysics, vol. 318, no. 1, (19950000), pages 25 - 29, URL: 10.1006/abbi.1995.1199, XP055514709
OPPOSITION- SÖDERBERG et al., "Monoclonal antibodies to human thioredoxin reductase.", Biochem. Bioph. Res. Co., (19980000), vol. 249, no. 1, XP055515274
OPPOSITION- TEILUM et al., "Disulfide bond formation and folding of plant peroxidases expressed as inclusion body protein in Escherichia coli thioredoxin reductase negative strains.", Protein Expres. Purif., (19990000), vol. 15, pages 77 - 82, XP004441744
OPPOSITION- TEILUM et al., "Disulfide Bond Formation and Folding of Plant Peroxidases Expressed as Inclusion Body Protein inEscherichia coliThioredoxin Reductase Negative Strains", Protein Expression and Purification, (19990000), vol. 15, pages 77 - 82, XP004441744
OPPOSITION- TEILUM K et al., "Disulfide Bond Formation and Folding of Plant Peroxidases Expressed as Inclusion Body Protein inEscherichia coliThioredoxin Reductase Negative Strains", Protein Expression and Purification, (19990000), vol. 15, pages 77 - 82, XP004441744
OPPOSITION- BROADWATER et al., "Spinach Holo-Acyl Carrier Protein: Overproduction and Phosphopantetheinylation inEscherichia coliBL21(DE3),in VitroAcylation, and Enzymatic Desaturation of Histidine-Tagged Isoform I", Prot. Exp. Purif., (19990000), vol. 15, pages 314 - 326, XP004441709
OPPOSITION- LEVY et al., "Production of Correctly Folded Fab Antibody Fragment in the Cytoplasm of Escherichia coli trxB gor Mutants via the Coexpression of Molecular Chaperones", Protein Expression and Purification, (20010000), vol. 23, pages 338 - 347, XP055024235
OPPOSITION- EMERY et al., "Oxygenation of intensive cell-culture system", Appl. Microbiol. Biotechnol., (19950000), vol. 43, pages 1028 - 1033, XP008123555
OPPOSITION- OBLONG et al., "Reversible inhibition of human thioredoxin reductase activity by cytotoxic alkyl 2-imidazolyl disulfide analogues", Cancer C hemother Pharmacol, (19940000), vol. 34, pages 434 - 438, XP009017128
OPPOSITION- YOON et al., "Effect of culture temperature on follicle-stimulating hormone production by Chinese hamster ovary cells in a perfusion bioreactor", Appl Microbiol Biotechnol, (20070000), vol. 76, no. 1, pages 83 - 89, XP019538802
OPPOSITION- THOMSEN et al., "The amino acid sequence of human glucagon", FEBS Letters, (19720000), vol. 21, no. 3, pages 315 - 319, XP055514759
OPPOSITION- CABILLY, S., "Growth at sub-optimal temperatures allows the production of functional, antigen-binding Fab fragments in Escherichia coli", Gene, (19890000), vol. 85, no. 2, pages 553 - 557, XP023544994
OPPOSITION- OH et al., "Thioredoxin secreted upon ultraviolet A irradiation modulates activities of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 in human dermal fibroblasts", Archives of Biochemistry and Biophysics, (20040000), vol. 423, pages 218 - 226, XP004488900
OPPOSITION- SCHWARTZ AG et al., "Dehydroepiandrosterone, glucose-6-phosphate dehydrogenase, and longevity", Ageing Research Reviews, (20040000), vol. 3, pages 171 - 187, XP055514707
OPPOSITION- CARTER-FRANKLIN et al., "Fragments of protein A eluted during protein A affinity chromatography", J. Chromatogr., (20070000), vol. 1163, pages 105 - 111, XP022202197
OPPOSITION- Boettcher, "Choosing the Right Tool for the Job: RNAi, TALEN, or CRISPR", Molecular Cell, vol. 58, no. 4, (20150521), pages 575 - 585, XP029129109
OPPOSITION- URIG et al., "On the potential of thioredoxin reductase inhibitors for cancer therapy", Seminars in Cancer Biology, (20060000), vol. 16, no. 6, pages 452 - 465, XP024908082
OPPOSITION- URIG et al., "On the potential of thioredoxin reductase inhibitors for cancer therapy", Seminars in Cancer Biology, (20060000), vol. 16, pages 452 - 465, XP005727270
OPPOSITION- URIG et al., "On the potential of thioredoxin reductase inhibitors for cancer therapy", Semin. Cancer Biol., (20060000), vol. 16, no. 6, pages 452 - 465, XP024908082
OPPOSITION- KIRKPATRICK et al., "Mechanisms of Inhibition of the Thioredoxin Growth Factor System by Antitumor 2-Imidazolyl Disulfides", Biochemical Pharmacology, (19980000), vol. 55, pages 987 - 994, XP002979943
OPPOSITION- POWIS et al., "Thioredoxin redox control of cell growth and death and the effects of inhibitors", Chem-Biol Interact., (19980000), pages 111 - 112, XP002519386
OPPOSITION- POWIS et al., "Thioredoxin redox control of cell growth and death and the effects of inhibitors", Chemico-Biological Interactions, (19980000), vol. 111-112, pages 23 - 34, XP002519386
OPPOSITION- POWIS et al., "Thioredoxin redox control of cell growth and death and the effects of inhibitors", Chemico-Biological Interactions, (19980000), vol. 111, no. 112, pages 23 - 34, XP002519386
OPPOSITION- Holmgren, "Reduction of disulfides by thioredoxin. Exceptional reactivity of insulin and suggested functions of thioredoxin in mechanism of hormone action", The Journal of biological chemistry, (19790000), vol. 254, pages 9113 - 9119, XP055515419
OPPOSITION- MAGNUSSON CGM et al., "Human IgG is substrate for the thioredoxin system: differential cleavage pattern of interchain disulfide bridges in IgG subclasses", Mol. Immunol., (19970000), vol. 34, pages 709 - 717, XP002497450
OPPOSITION- MAGNUSSON et al., "Human IgG is substrate for the thioredoxin system: differential cleavage pattern of interchain disulfide bridges in IgG subclasses", Molecular immunology, (19970000), vol. 34, no. 10, pages 709 - 717, XP002497450
OPPOSITION- MAGNUSSON et al., "Human IgG is substrate for the thioredoxin system: differential cleavage pattern of interchain disulfide bridges in IgG subclasses", Molecular Immunology, (19970000), vol. 34, no. 10, pages 709 - 717, XP002497450
OPPOSITION- MAGNUSSON et al., "Human IgG is substrate for the thioredoxin system: differential cleavage pattern of interchain disulfide bridges in IgG subclasses", MOLECULAR IMMUNOLOGY, vol. 34, no. 10, (19970701), pages 709 - 717, XP002497450
OPPOSITION- MAGNUSSON et al., "Human IgG is substrate for the thioredoxin system: differential cleavage pattern of interchain disulfide bridges in IgG subclasses", Mol. Immunol., (19970000), vol. 34, no. 10, pages 709 - 717, XP002497450
OPPOSITION- NORDBERG et al., "Reactive oxygen species, antioxidants, and the mammalian thioredoxin system", Free Radical Biology & Medicine, (20010000), vol. 31, no. 11, pages 1287 - 1312, XP002519388
OPPOSITION- NORDBERG et al., "Reactive oxygen species, antioxidants, and the mammalian thioredoxin system", Free Radical Bio. Med., (20010000), vol. 31, no. 11, pages 1287 - 1312, XP002519388
OPPOSITION- NORDBERG J et al., "Reactive oxygen species, antioxidants, and the mammalian thioredoxin system", Free Radical Biology & Medicine, (20010000), vol. 31, no. 11, pages 1287 - 1312, XP002519388
OPPOSITION- WIPF et al., "New Inhibitors of the Thioredoxin-Thioredoxin Reductase System Based on a Naphthoquinone Spiroketal Natural Product Lead", Bioorganic and Medicinal Chemistry Letters, (20010000), vol. 11, pages 2637 - 2641, XP055514897
OPPOSITION- WIPF et al., "New inhibitors of the thioredoxin-thioredoxin reductase system based on a naphthoquinone spiroketal natural product lead", Bioorgan. Med. Chem. Lett., (20010000), vol. 11, pages 2637 - 2641, XP055514897
OPPOSITION- HOLMGREN, "Thioredoxin structure and mechanism: conformational changes on oxidation of the active-side sulfhydryls to a disulfide", Minireview, Structure, (19950000), vol. 3, no. 3, pages 239 - 243, XP004587846
OPPOSITION- GLOCKSHUBER et al., "The disulfide bonds in antibody variable domains: effects on stability, folding in vitro, and functional expression in Escherichia coli", Biochemistry, (19920000), vol. 31, pages 1270 - 1279, XP009100444
OPPOSITION- Zhang et al., "Free sulfhydryl in recombinant monoclonal antibodies", BIOTECHNOLOGY PROGRESS,, vol. 18, (20020501), pages 509 - 513, XP002500112
OPPOSITION- ZHANG et al., "Free sulfhydryl in recombinant monoclonal antibodies", Biotechnol. Prog, (20020000), vol. 18, pages 509 - 513, XP002500112
OPPOSITION- Z HANG et al., "Free Sulfhydryl in Recombinant Monoclonal Antibodies", Biotechnol. Prog., (20020000), vol. 18, pages 509 - 513, XP002500112
OPPOSITION- ZHANG et al., "Free sulfhydryl in recombinant monoclonal antibodies", Biotechnol. Prog., (20020500), vol. 18, pages 509 - 513, XP002500112
OPPOSITION- ZHANG W et al., "Free sulfhydryl in recombinant monoclonal antibodies", Biotechnol. Prog., (20020000), vol. 18, pages 509 - 513, XP002500112
OPPOSITION- Chaderjian, "Effect of Copper Sulfate on Performance of a Serum-Free CHO Cell Culture Process and the Level of Free Thiol in the Recombinant Antibody Expressed", Biotechnol. Prag., (20050300), vol. 21, pages 550 - 553, XP009107034
OPPOSITION- CHADERJIAN et al., "Effect of Copper Sulfate on Performance of a Serum-Free CHO Cell Culture Process and the Level of Free Thiol in the Recombinant Antibody Expressed", Biotechnol Prog, (20050300), vol. 21, no. 2, pages 550 - 553, XP009107034
OPPOSITION- CHADERJIAN et al., "Effect of copper sulfate on performance of a serum-free CHO cell culture process and the level of free thiol in the recombinant antibody expressed", Biotechnology Progress, (20050000), vol. 21, pages 550 - 553, XP002500111
OPPOSITION- CHADERJIAN et al., "Effect of copper sulfate on performance of a serum-free CHO cell culture process and the level of free thiol in the recombinant antibody expressed", Biotechnol. Prog., (20050000), vol. 21, no. 2, pages 550 - 553, XP002500111
OPPOSITION- CHADERJIAN et al., "Effect of copper sulfate on performance of a serum-free CHO cell culture process and the level of free thiol in the recombinant antibody expressed", Biotechnol. Prog., (20050000), vol. 21, pages 550 - 553, XP002500111
OPPOSITION- CHADERJIAN WB et al., "Effect of copper sulfate on performance of a serum-free CHO cell culture process and the level of free thiol in the recombinant antibody expressed", Biotechnol. Prog., (20050000), vol. 21, pages 550 - 553, XP002500111
OPPOSITION- YIGZAW et al., "Exploitation of the adsorptive properties of depth filters for host cell protein removal during monoclonal antibody purification", Biotechnol. Prog, (20060000), vol. 22, pages 288 - 296, XP002472117
OPPOSITION- ROUSH et al., "Advances in Primary Recovery: Centrifugation and Membrane Technology", Biotechnol. Prog., (20080000), vol. 24, no. 3, pages 488 - 495, XP055090116
OPPOSITION- ROUSH et al., "Advances in Primary Recovery: Centrifugation and Membrane Technology", Biotechnol. Prog., (20080000), vol. 24, pages 488 - 495, XP055090116
OPPOSITION- J .S.WALL, "Disulfide Bonds: Determination, Location, and Influence on Molecular Properties of Proteins", J Agri Food Chem, (19710000), vol. 19, pages 619 - 625, XP055514799
OPPOSITION- MUKHERJEE et al., "Cytotoxic and antiangiogenic activity of AW464 (NSC 706704), a novel thioredoxin inhibitor: an in vitro study", British Journal of Cancer, (20050000), vol. 92, pages 350 - 358, XP055514891
OPPOSITION- RAMIREZ et al., "Production, recovery and immunogenicity of the protective antigen from a recombinant strain of Bacillus anthracis.", J. Indust. Microbiol. Biotechnol., (20020000), vol. 28, pages 232 - 238, XP055514727
OPPOSITION- KERBLAT et al., "Importance of thioredoxin in the proteolysis of an immunoglobulin G as antigen by lysosomal Cys-proteases", Immunology, (19990000), vol. 97, pages 62 - 68, XP002519384
OPPOSITION- KERBLAT et al., "Importance of thioredoxin in the proteolysis of an immunoglobulin G as antigen by lysosomal Cys-proteases", Immunology, (19990500), vol. 97, no. 1, pages 62 - 68, XP002519384
OPPOSITION- KERBLAT I et al., "Importance of thioredoxin in the proteolysis of an immunoglobulin G as antigen by lysosomal Cys-proteases", Immunology, (19990000), vol. 97, no. 1, pages 62 - 68, XP002519384
OPPOSITION- WATABE S et al., "Mitochondrial thioredoxin reductase in bovine adrenal cortexIts purification, properties, nucleotide/amino acid sequences, and identification of selenocysteine", Eur. J. Biochem., (19990000), vol. 264, pages 74 - 84, XP055514705
OPPOSITION- PRINZ et al., "The role of the thioredoxin and glutaredoxin pathways in reducing protein disulfide bonds in the Escherichia coli cytoplasm", J. Biological Chemistry, (19970000), vol. 272, no. 25, pages 15661 - 15667, XP002515937
OPPOSITION- TIAN WN, BRAUNSTEIN LD et al., "Importance of Glucose-6-phosphate Dehydrogenase Activity for Cell Growth", JBC, (19980000), vol. 273, no. 17, pages 10609 - 10617, XP055514719
OPPOSITION- JOHANSSON C, "Human mitochondrial glutaredoxin reduces S-glutathionylated proteins with high affinity accepting electrons from either glutathione or thioredoxin reductase.", JBC, (20040000), vol. 279, no. 9, pages 7537 - 7543, XP055514721
OPPOSITION- FAHRNER et al., "Industrial Purification of Pharmaceutical Antibodies: Development, Operation, and Validation of Chromatography Processes", Biotechnology and Genetic Engineering Rev., (20010000), vol. 18, pages 301 - 327, XP003035628
OPPOSITION- SMITH et al., "Aurothioglucose inhibits murine thioredoxin reductase activity in vivo", American Society for Nutritional Sciences, (19990000), vol. 129, no. 1, pages 194 - 198, XP055514900
OPPOSITION- SMITH et al., "Aurothioglucose inhibits murine thioredoxin reductase activity in vivo.", J. Nutr., (19990000), vol. 129, pages 194 - 198, XP055514900
OPPOSITION- LYDERSEN et al., "Acid precipitation of mammalian cell fermentation broth.", Annals of the New York Academy of Sciences, (19941130), vol. 745, no. 1, pages 222 - 231, XP009033271
OPPOSITION- LYDERSEN et al., "Acid precipitation of mammalian cell fermentation broth", Ann. NY Acad. Sci., (19940000), vol. 745, no. 1, pages 222 - 231, XP009033271
OPPOSITION- SINGH et al., "Biochemical and Immunological Characterization of Bacterially Expressed and Refolded Plasmodium falciparum 42-Kilodalton C-Terminal Merozoite Surface Protein 1", Infection and Immunity, (20031200), pages 6766 - 6774, XP055028227
OPPOSITION- POWIS et al., "Properties and biological activities of thioredoxins", Annual Review of Pharmacology and Toxicology, (20010000), vol. 41, pages 261 - 295, XP002519387
OPPOSITION- POWIS et al., "Properties and biological activities of thioredoxins", Annu. Rev. Pharmacol. Toxicol., (20010000), vol. 41, pages 261 - 295, XP002519387
OPPOSITION- BRAGADIN et al., "Effect of Metal Complexes on Thioredoxin Reductase and the Regulation of Mitochondrial Permeability Conditions", Annals of the New York Academy of Sciences, (20040000), vol. 1030, pages 348 - 354, XP055514905
OPPOSITION- CROMWELL et al., "Protein Aggregation and Bioprocessing", The AAPS Journal, (20060000), vol. 8, no. 3, pages E572 - E579, XP035718852
OPPOSITION- CROMWELL MEM et al., "Protein aggregation and bioprocessing", AAPSJ, (20060000), vol. 8, pages E572 - E579, XP035718852
OPPOSITION- BOBOVNIKOVA et al., "Characterization of soluble, disulfide bond-stabilized, prokaryotically depressed human thyrotropin receptor ectodomain", Endocrinology, (19960000), vol. 138, no. 2, pages 588 - 593, XP002519385
OPPOSITION- BOBOVNIKOVA et al., "Characterization of soluble, disulfide bond-stabilized, prokaryotically depressed human thyrotropin receptor ectodomain", Endocrinology, (19970000), vol. 138, no. 2, pages 588 - 593, XP002519385
OPPOSITION- BOBOVNIKOVA Y et al., "Characterization of soluble, disulfide bond-stabilized, prokaryotically depressed human thyrotropin receptor ectodomain", Endocrinology, (19970000), vol. 138, no. 2, pages 588 - 593, XP002519385
OPPOSITION- BIGALOW et al., "The thioredoxin reductase/thioredoxin system:Novel redox targets for cancer therapy", Cancer Biol. Ther., (20050000), vol. 4, no. 1, pages 13 - 20, XP055515262
OPPOSITION- HUTTERER et al., "Monoclonal antibody disulfide reduction during manufacturing: Untangling process effects from product effects.", mAbs, (20130000), vol. 5, no. 4, pages 608 - 613, XP055514733
OTHER- ROUSH D.J. ET AL, "Advances in Primary Recovery: Centrifugation and Membrane Technology", BIOTECHNOL. PROG., (20080606), vol. 24, pages 488 - 495, XP055090116
OTHER- LYDERSEN B.K. ET AL, "ACID PRECIPITATION OF MAMMALIAN CELL FERMENTATION BROTH", ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, (19941130), vol. 745, pages 222 - 231, XP009033271

Download Citation Report

Get a free citation report including examiner, opposition, and international search citations.

Get Citation Report

Dossier Documents

The dossier documents provide a comprehensive record of the patent’s prosecution history - including filings, correspondence, and decisions made by patent offices - and are crucial for understanding the patent’s legal journey and any challenges it may have faced during examination.

  • Date

    Description

  • Get instant alerts for new documents