Methods Of Detecting Nucleic Acids In Individual Cells And Of Identifying Rare Cells From Large Heterogeneous Cell Populations - EP2500439

The patent EP2500439 was granted to Advanced Cell Diagnostics on Aug 16, 2017. The application was originally filed on Jun 19, 2006 under application number EP12161252A. The patent is currently recorded with a legal status of "Patent Maintained As Amended".

EP2500439

ADVANCED CELL DIAGNOSTICS
Application Number
EP12161252A
Filing Date
Jun 19, 2006
Status
Patent Maintained As Amended
Jul 14, 2017
Publication Date
Aug 16, 2017
External Links
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AFFYMETRIXApr 27, 2015BOULT WADE TENNANTADMISSIBLE

Patent Citations (24) New

Patent citations refer to prior patents cited during different phases such as opposition or international search.

Citation PhasePublication Number
DESCRIPTIONUS2002172950
DESCRIPTIONUS2004091880
DESCRIPTIONUS2005181463
DESCRIPTIONUS3817837
DESCRIPTIONUS3850752
DESCRIPTIONUS3939350
DESCRIPTIONUS3996345
DESCRIPTIONUS4275149
DESCRIPTIONUS4277437
DESCRIPTIONUS4366241
DESCRIPTIONUS5124246
DESCRIPTIONUS5635352
DESCRIPTIONUS5681697
DESCRIPTIONUS5710264
DESCRIPTIONUS5849481
DESCRIPTIONUS6001983
DESCRIPTIONUS6037120
DESCRIPTIONUS6140496
DESCRIPTIONUS6365362
DESCRIPTIONUS6645731
DESCRIPTIONWO03019141
DESCRIPTIONWO9509245
OPPOSITIONUS5635352
SEARCHUS2002172950

Non-Patent Literature (NPL) Citations (62) New

NPL citations refer to non-patent references such as research papers, articles, or other publications cited during examination or opposition phases.

Citation PhaseReference Text
DESCRIPTION- BOLAND, "Advances in Colorectal Cancer Screening: Molecular Basis for Stool-Based DNA Tests for Colorectal Cancer: A Primer for Clinicans", REVIEWS IN GASTROENTEROLOGICAL DISORDERS, (2002), vol. 2, no. 1
DESCRIPTION- BRAISSANT; WAHLI, "A simplified in situ hybridization protocol using non- radioactively labeled probes to detect abundant and rare mRNAs on tissue sections", BIOCHEMICA, (1988), vol. 1, pages 10 - 16
DESCRIPTION- CSAKI ET AL., "Gold nanoparticles as novel label forDNA diagnostics", EXPERT REV MOL DIAGN, (2002), vol. 2, pages 187 - 93
DESCRIPTION- DUBERTRET ET AL., SCIENCE, (2002), vol. 298, page 1759
DESCRIPTION- FAVA TA ET AL., "Ectopic expression of guanylyl cyclase C in CD34+ progenitor cells in peripheral blood", J CLIN ONCOL., (20011001), vol. 19, no. 19, pages 3951 - 9
DESCRIPTION- GEWANTER, R. M.; A. E. KATZ ET AL., "RT-PCR for PSA as a prognostic factor for patients with clinically localized prostate cancer treated with radiotherapy", UROLOGY, (2003), vol. 61, no. 5, pages 967 - 71
DESCRIPTION- GIATROMANOLAKI ET AL., "Assessment of highly angiogenic and disseminated in the peripheral blood disease in breast cancer patients predicts for resistance to adjuvant chemotherapy and early relapse", INT J CANCER, (2004), vol. 108, no. 4, pages 620 - 7
DESCRIPTION- "Guide to Molecular Cloning Techniques", BERGER; KIMMEL, Methods in Enzymology, ACADEMIC PRESS, INC., vol. 152
DESCRIPTION- HALABI ET AL., "Prognostic significance of reverse transcriptase polymerase chain reaction for prostate-specific antigen in metastatic prostate cancer: a nested study within CALGB 9583", J CLIN ONCOL, (2003), vol. 21, no. 3, pages 490 - 5
DESCRIPTION- HARDINGHAM ET AL., "Molecular detection of blood-borne epithelial cells in colorectal cancer patients and in patients with benign bowel disease", INT J CANCER, (2000), vol. 89, no. 1, pages 8 - 13
DESCRIPTION- HAYES ET AL., "Monitoring expression of HER-2 on circulating epithelial cells in patients with advanced breast cancer", INT J ONCOL, (2002), vol. 21, no. 5, pages 1111 - 7, XP002451689
DESCRIPTION- HERZENBERG LA ET AL., "The history and future of the fluorescence activated cell sorter and flow cytometry: a view from Stanford", CLIN CHEM., (200210), vol. 48, no. 10, pages 1819 - 27, XP002485814
DESCRIPTION- HESS CJ ET AL., "Gene expression profiling of minimal residual disease in acute myeloid leukaemia by novel multiplex-PCR-based method", LEUKEMIA, (200412), vol. 18, no. 12, pages 1981 - 8
DESCRIPTION- HICKS DG ET AL., "In situ hybridization in the pathology laboratory: General principles, automation, and emerging research applications for tissue-based studies of gene expression", J MOL HISTOL., (200408), vol. 35, no. 6, pages 595 - 601
DESCRIPTION- ITO S ET AL., "Quantitative detection of CEA expressing free tumor cells in the peripheral blood of colorectal cancer patients during surgery with the real-time RT-PCR on a Light Cycler", CANCER LETTERS, (2002), vol. 183, pages 195 - 203
DESCRIPTION- JOTSUKA ET AL., "Persistent evidence of circulating tumor cells detected by means of RT-PCR for CEA mRNA predicts early relapse: a prospective study in node-negative breast cancer", SURGERY, (2004), vol. 135, no. 4, pages 419 - 26
DESCRIPTION- KENNY ET AL., J. HISTOCHEM. CYTOCHEM., (2002), vol. 50, pages 1219 - 1227
DESCRIPTION- MA PC ET AL., "Circulating tumor cells and serum tumor biomarkers in small cell lung cancer", ANTICANCER RES., (200301), vol. 23, no. LA, pages 49 - 62, XP009103203
DESCRIPTION- NATURE BIOTECHNOLOGY, (2003), vol. 21, pages 41 - 46
DESCRIPTION- NATURE BIOTECHNOLOGY, (2003), vol. 21, pages 47 - 51
DESCRIPTION- NILSSON ET AL., SCIENCE, (1994), vol. 265, pages 2085 - 2088
DESCRIPTION- PLAYER AN; SHEN LP; KENNY D; ANTAO VP; KOLBERG JA, "Single-copy gene detection using branched DNA (bDNA) in situ hybridization", J HISTOCHEM CYTOCHEM., (200105), vol. 49, no. 5, pages 603 - 12, XP001041855
DESCRIPTION- PLAYER ET AL., J. HISTOCHEM. CYTOCHEM., (2001), vol. 49, pages 603 - 611
DESCRIPTION- RUFER N, "Telomere length dynamics in human lymphocyte subpopulations measured by flow cytometry", NAT BIOTECHNOL., (199808), vol. 16, no. 8, pages 743 - 7
DESCRIPTION- SAMBROOK ET AL., Molecular Cloning - A Laboratory Manual, COLD SPRING HARBOR LABORATORY, (2000), vol. 1-3
DESCRIPTION- SANTALUCIA JR., PROC NATL ACAD SCI, (1998), vol. 95, pages 1460 - 1465
DESCRIPTION- SHARIAT ET AL., "Early postoperative peripheral blood reverse transcription PCR assay for prostate-specific antigen is associated with prostate cancer progression in patients undergoing radical prostatectomy", CANCER RES, (2003), vol. 63, no. 18, pages 5874 - 8
DESCRIPTION- SMITH ET AL., "Response of circulating tumor cells to systemic therapy in patients with metastatic breast cancer: comparison of quantitative polymerase chain reaction and immunocytochemical techniques", J CLIN ONCOL, (2000), vol. 18, no. 7, pages 1432 - 9
DESCRIPTION- "The Genetics of Colorectal Cancer", CALVERT ET AL., Annals of Internal Medicine, (2002), vol. 137, pages 603 - 612
DESCRIPTION- TIMM EA JR ET AL., "Amplification and detection of a Y-chromosome DNA sequence by fluorescence in situ polymerase chain reaction and flow cytometry using cells in suspension", CYTOMETRY, (19950915), vol. 22, no. 3, pages 250 - 5
DESCRIPTION- TIMM EA JR; STEWART CC, "Fluorescent in situ hybridization en suspension (FISHES) using digoxigenin-labeled probes and flow cytometry", BIOTECHNIQUES, (199203), vol. 12, no. 3, pages 362 - 7
DESCRIPTION- VLEMS FA ET AL., "Detection and clinical relevance of tumor cells in blood and bone marrow of patients with colorectal cancer", ANTICANCER RES., (200301), vol. 23, no. LB, pages 523 - 30
DESCRIPTION- VOGEL I ET AL., "Detection and prognostic impact of disseminated tumor cells in pancreatic carcinoma", PANCREATOLOGY, (2002), vol. 2, no. 2, pages 79 - 88
DESCRIPTION- YEN, "Physiological and Molecular Basis of Thyroid Honnone Action", PHYSIOLOGICAL REVIEWS, (2001), vol. 81, no. 3, pages 1097 - 1142, XP009099773
DESCRIPTION- YU ET AL., "Sensitive detection of RNAs in single cells by flow cytometry", NUCLEIC ACIDS RES., (1992), vol. 20, no. 1, pages 83 - 88
DESCRIPTION- BAUMAN; BENTVELZEN, "Flow cytometric detection of ribosomal RNA in suspended cells by fluorescent in situ hybridization", CYTOMETRY, (1988), vol. 9, no. 6, doi:doi:10.1002/cyto.990090602, pages 517 - 524, XP009084761
DESCRIPTION- BAUMAN JG; BENTVELZEN P, "Flow cytometric detection of ribosomal RNA in suspended cells by fluorescent in situ hybridization", CYTOMETRY, (198811), vol. 9, no. 6, doi:doi:10.1002/cyto.990090602, pages 517 - 24, XP009084761
DESCRIPTION- MOCELLIN S ET AL., "Molecular detection of circulating tumor cells in an independent prognostic factor in patients with high-risk cutaneous melanoma", INT J CANCER, (2004), vol. 111, doi:doi:10.1002/ijc.20347, pages 741 - 745, XP002628788
DESCRIPTION- MOSIMAN ET AL., "Reducing cellular autofluorescence in flow cytometry: an in situ method", CYTOMETRY, (1997), vol. 30, no. 3, doi:doi:10.1002/(SICI)1097-0320(19970615)30:3<151::AID-CYTO6>3.0.CO;2-O, pages 151 - 156, XP002909760
DESCRIPTION- BAINS MA, "Flow cytometric quantitation of sequence-specific mRNA in hemopoietic cell suspensions by primer-induced in situ (PRINS) fluorescent nucleotide labeling", EXP CELL RES., (199309), vol. 208, no. 1, doi:doi:10.1006/excr.1993.1252, pages 321 - 6, XP024788903
DESCRIPTION- MA ET AL., "A two-gene expression ratio predicts clinical outcome in breast cancer patients treated with tamoxifen", CANCER CELL, (2004), vol. 5, no. 6, doi:doi:10.1016/j.ccr.2004.05.015, pages 607 - 616, XP002317299
DESCRIPTION- NOLAN ET AL., "A simple quenching method for fluorescence background reduction and its application to the direct, quantitative detection of specific mRNA", ANAL CHEM., (2003), vol. 75, no. 22, doi:doi:10.1021/ac034803r, pages 6236 - 6243, XP003017563
DESCRIPTION- LARSSON C; KOCH J; NYGREN A; JANSSEN G; RAAP AK; LANDEGREN U; NILSSON M, "In situ genotyping individual DNA molecules by target-primed rolling-circle amplification of padlock probes", NAT METHODS., (20041118), vol. 1, no. 3, doi:doi:10.1038/nmeth723, pages 227 - 32, XP008154567
DESCRIPTION- LARSSON ET AL., "In situ genotyping individual DNA molecules by target-primed rolling-circle amplification of padlock probes", NAT METHODS., (2004), vol. 1, no. 3, doi:doi:10.1038/nmeth723, pages 227 - 232, XP008154567
DESCRIPTION- CAPODIECI ET AL., "Gene expression profiling in single cells within tissue", NAT METHODS, (2005), vol. 2, no. 9, doi:doi:10.1038/nmeth786, pages 663 - 665, XP009070911
DESCRIPTION- LI ET AL., "BEAMing up for detection and quantification of rare sequence variants", NATURE METHODS, (2006), vol. 3, no. 2, doi:doi:10.1038/nmeth850, pages 95 - 97, XP008126872
DESCRIPTION- CRISTOFANILLI M. ET AL., "Circulating tumor cells, disease progression, and survival in metastatic breast cancer", N ENGL J MED., (20040819), vol. 351, no. 8, doi:doi:10.1056/NEJMoa040766, pages 781 - 91, XP002458149
DESCRIPTION- XENIDIS ET AL., "Peripheral blood circulating cytokeratin-19 mRNA-positive cells after the completion of adjuvant chemotherapy in patients with operable breast cancer", ANN ONCOL, (2003), vol. 14, no. 6, doi:doi:10.1093/annonc/mdg259, pages 849 - 55, XP002407127
DESCRIPTION- HULTDIN M, "Telomere analysis by fluorescence in situ hybridization and flow cytometry", NUCLEIC ACIDS RES., (19980815), vol. 26, no. 16, doi:doi:10.1093/nar/26.16.3651, pages 3651 - 6, XP002978619
DESCRIPTION- ANTSON ET AL., "PCR-generated padlock probes detect single nucleotide variation in genomic DNA", NUCL ACIDS RES, (2000), vol. 28, no. 12, doi:doi:10.1093/nar/28.12.e58, page E58, XP002302312
DESCRIPTION- KOSMAN D; MIZUTANI CM; LEMONS D; COX WG; MCGINNIS W; BIER E., "Multiplex detection of RNA expression in Drosophila embryos", SCIENCE, (20040806), vol. 305, no. 5685, doi:doi:10.1126/science.1099247, page 846, XP002394668
DESCRIPTION- SCHROCK E; DU MANOIR S; VELDMAN T; SCHOELL B; WIENBERG J; FERGUSON-SMITH MA; NING Y; LEDBETTER DH; BAR-AM I; SOENKSEN D, "Multicolor spectral karyotyping of human chromosomes", SCIENCE, (19960726), vol. 273, no. 5274, doi:doi:10.1126/science.273.5274.494, pages 494 - 7, XP002110739
DESCRIPTION- PATTERSON BK, "Detection of HIV-1 DNA and messenger RNA in individual cells by PCR- driven in situ hybridization and flow cytometry", SCIENCE, (19930514), vol. 260, no. 5110, doi:doi:10.1126/science.8493534, pages 976 - 9, XP000612079
DESCRIPTION- GILBEY AM ET AL., "The detection of circulating breast cancer cells in blood", J CLIN PATHOL., (200409), vol. 57, no. 9, doi:doi:10.1136/jcp.2003.013755, pages 903 - 11, XP055028756
DESCRIPTION- GOETZ ET AL., "A Two-Gene Expression Ratio of Homeobox 13 and Interleukin-17B Receptor for Prediction of Recurrence and Survival in Women Receiving Adjuvant Tamoxifen", CLIN CANCER RES, (2006), vol. 12, doi:doi:10.1158/1078-0432.ccr-05-1263, pages 2080 - 2087, XP002572497
DESCRIPTION- MOLNAR B ET AL., "Molecular detection of circulating cancer cells. Role in diagnosis, prognosis and follow-up of colon cancer patients", DIG DIS., (2003), vol. 21, no. 4, doi:doi:10.1159/000075355, pages 320 - 5, XP008078262
DESCRIPTION- STATHOPOULOU ET AL., "Molecular detection of cytolceratin-19-positive cells in the peripheral blood of patients with operable breast cancer: evaluation of their prognostic significance", J CLIN ONCOL, (2002), vol. 20, no. 16, doi:doi:10.1200/JCO.2002.08.135, pages 3404 - 12, XP002407126
OPPOSITION- COLLINS, M.L. ET AL, "A branched DNA signal amplification assay for quantification of nucleic acid targets below 100 molecules/ml", NUCLEIC ACIDS RESEARCH, (1997), vol. 25, no. 15, pages 2979 - 2984
SEARCH- HARTLEY D P ET AL, "DETECTION OF CHEMICAL-INDUCED DIFFERENTIAL EXPRESSION OF RAT HEPATIC CYTOCHROME P450 MRNA TRANSCRIPTS USING BRANCHED DNA SIGNAL AMPLIFICATION TECHNOLOGY", DRUG METABOLISM AND DISPOSITION, WILLIAMS AND WILKINS, BALTIMORE, MD, US, (20000101), vol. 28, no. 5, ISSN 0090-9556, pages 608 - 616, XP002945499 [A] 1-28 * abstract *
SEARCH- KENNY D ET AL, "Detection of viral infection and gene expression in clinical tissue specimens using branched DNA (bDNA) in situ hybridization", JOURNAL OF HISTOCHEMISTRY AND CYTOCHEMISTRY, HISTOCHEMICAL SOCIETY, NEW YORK, NY, US, (20020901), vol. 50, no. 9, ISSN 0022-1554, pages 1219 - 1227, XP002390589 [A] 1-28 * the whole document *
SEARCH- PLAYER AUDREY N ET AL, "Single-copy gene detection using branched DNA (bDNA) in situ hybridization", JOURNAL OF HISTOCHEMISTRY AND CYTOCHEMISTRY, (200105), vol. 49, no. 5, ISSN 0022-1554, pages 603 - 611, XP001041855 [A] 1-28 * the whole document *
SEARCH- FLAGELLA M ET AL, "A multiplex branched DNA assay for parallel quantitative gene expression profiling", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS INC, NEW YORK, vol. 352, no. 1, ISSN 0003-2697, (20060501), pages 50 - 60, (20060501), XP024942379 [T] 1-28 * the whole document *

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