Adapted Lepidopteran Insect Cells For The Production Of Recombinant Proteins - EP2970916

The patent EP2970916 was granted to Merck Sharp & Dohme on Apr 14, 2021. The application was originally filed on Mar 13, 2014 under application number EP14775102A. The patent is currently recorded with a legal status of "Revoked".

EP2970916

MERCK SHARP & DOHME
Application Number
EP14775102A
Filing Date
Mar 13, 2014
Status
Revoked
Jan 17, 2025
Publication Date
Apr 14, 2021
External Links
Slate, Register, Google Patents

Patent Summary

Patent Family

Patent Family

Patent Oppositions (2)

Patent oppositions filed by competitors challenge the validity of a granted patent. These oppositions are typically based on claims of prior art, lack of novelty, or non-obviousness. They are a key part of the process for determining a patent's strength and enforceability.

CompanyOpposition DateRepresentativeOpposition Status

Get instant alerts for new oppositions and patent status changes

BOEHRINGER INGELHEIM VETMEDICAJan 14, 2022D YOUNGADMISSIBLE
STRAWMANJan 14, 2022GREAVES BREWSTERADMISSIBLE

Patent Citations (25) New

Patent citations refer to prior patents cited during different phases such as opposition or international search.

Citation PhasePublication Number
INTERNATIONAL-SEARCH-REPORTUS2013344084
OPPOSITIONCN102634476
OPPOSITIONCN1124163
OPPOSITIONCN1962857
OPPOSITIONEP1482031
OPPOSITIONJPS63148982
OPPOSITIONJPS63148983
OPPOSITIONUS2002127582
OPPOSITIONUS2004259150
OPPOSITIONUS2009068741
OPPOSITIONUS2011086413
OPPOSITIONUS2011117087
OPPOSITIONUS2013344084
OPPOSITIONUS201361779845
OPPOSITIONUS201361792613
OPPOSITIONUS5024947
OPPOSITIONUS6767741
OPPOSITIONWO0166696
OPPOSITIONWO02066603
OPPOSITIONWO2008033517
OPPOSITIONWO8901029
OPPOSITIONWO9100341
OPPOSITIONWO9404660
OPPOSITIONWO9808934
SEARCHWO0166696

Non-Patent Literature (NPL) Citations (66) New

NPL citations refer to non-patent references such as research papers, articles, or other publications cited during examination or opposition phases.

Citation PhaseReference Text
EXAMINATION- G M Lee ET AL, "Serum Can Act as a Shear Protecting Agent in Agitated Hybridoma Cell Cultures", Hybridoma, United States, doi:10.1089/hyb.1989.8.639, (19891201), pages 639 - 645, URL: https://www.liebertpub.com/doi/pdf/10.1089/hyb.1989.8.639, (20180720), XP055492831
EXAMINATION- HSU S H ET AL, "A CELL LINE DERIVED FROM OVARIAN TISSUE OF CULEX-TRITAENIORHYNCHUS-SUMMOROSUS", JOURNAL OF MEDICAL ENTOMOLOGY, ENTOMOLOGICAL SOCIETY OF AMERICA, LANHAM, MD, US, (19720101), vol. 9, no. 1, ISSN 0022-2585, pages 86 - 91, XP009195277
INTERNATIONAL-SEARCH-REPORT- GAIGE, D., "TNMFH Insect Media.xls.", PDF DOCUMENT., (20030326), URL: http://ebookbrowsee.net/tnmfh-insect-media-pdf-d298031253, (20140527), XP055280720
INTERNATIONAL-SEARCH-REPORT- KOVAL ET AL., "PH Dependency of Cell Attachment and Growth at Both Clonal and Subculture Densities of Cultured Lepidopteran Cells.", IN VITRO CELL . DEV. BIOL., (199007), vol. 26, no. 7, pages 665 - 670 ., XP008180965
INTERNATIONAL-SEARCH-REPORT- KUO ET AL., "Cell -based analysis of Chikungunya virus E1 protein in membrane fusion.", J BIOMED SCI., (20120421), vol. 19, no. 44, pages 1 - 12, XP055280716
INTERNATIONAL-SEARCH-REPORT- METZ ET AL., "Functional processing and secretion of Chikungunya virus E1 and E2 glycoproteins in insect cells.", J VIROL, (20110715), vol. 8, no. 353, pages 1 - 12, XP021105754
INTERNATIONAL-SEARCH-REPORT- IKONOMOU ET AL., "Design of an Efficient medium for Insect Cell Growth and Recombinant Protein Production.", IN VITRO CELL . DEV. BIOL. (ANIMAL, (200110), vol. 37, no. 9, pages 549 - 559, XP009058879
INTERNATIONAL-SEARCH-REPORT- METZ ET AL., "Effective Chikungunya Virus-like Particle Vaccine Produced in Insect Cells.", PLOS NEGL TROP DIS., (20130314), vol. 7, no. 3, pages 1 - 11, XP055280724
OPPOSITION- AGATHOS, S. et al., "Insect Cell Culture", AGATHOS, S. et al., Richard H. B., Julian E. Davies, ASM Press, (20100000), XP055889321
OPPOSITION- Anonymous, "11825 - Medium 199, 10X", ThermoFisher - Technical Resource, URL: https://www.thermofisher.com/nl/en/home/technical-resources/media-formulation.89.html
OPPOSITION- Anonymous, "12350 - Medium 199, Hank's", ThermoFisher - Technical Resources, (20160418), ThermoFisher - Technical Resources, URL: https://www.thermofisher.com/nl/en/home/technical-resources/media-formulation.91.html, (20220209), XP055889313
OPPOSITION- Anonymous, "DMEM/F12 Complete with 2mM L-Glut and 10% FBS", Merck, (2021), URL: https://www.sigmaaldrich.com/GB/en/product/mm/slm243b, XP055889312
OPPOSITION- Anonymous, "Dulbecco's Modified Eagle's Medium/ Ham's Nutrient Mixture F-12", SAFC Biosciences - Product Information, (20061200), XP055889318
OPPOSITION- Anonymous, "Growth and Maintenance of Insect Cell Lines", Invitrogen, (20020000), URL: www.invitrogen.com, XP055889332
OPPOSITION- Anonymous, "Hank's Balanced Salts", Sigma-Aldrich - Product Information, (20070000), XP055889317
OPPOSITION- Anonymous, "IPL-41 Insect Medium Formulation", ThermoFisher - Technical Resources, URL: https://www.thermofisher.com/nl/en/home/technical-resources/media-formulation.75.html
OPPOSITION- Anonymous, "TNM-FH Insect Medium With L-Glutamine, Lactalbumin hydrolysate and Yeast Extract Without Sodium bicarbonate Product Code: IM008", TNM-FH - Product Information, (20130101), pages 1 - 2, TNM-FH - Product Information, URL: https://himedialabs.com/TD/IM008.pdf, (20220208), XP055888759
OPPOSITION- Arthur H. Mcintosh, K. Maramorosch And C. Rechtoris, "Adaptation of an Insect Cell Line (Agallia constricta) in a Mammalian Cell Culture Medium", In Vitro, (19730101), vol. 8, no. 5, pages 375 - 378, XP055889311
OPPOSITION- BEAS-CATENA et al., "Adaptation of the Se301 insect cell line to suspension culture. Effect of turbulence on growth and on production of nucleopolyhedrovius (SeMNPV)", Cytotechnology, (20110000), vol. 63, pages 543 - 552, XP055892017
OPPOSITION- BROEDEL et al., "The Case for Serum-Free Media", BioProcess International, (20030000), pages 57 - 58, XP055892051
OPPOSITION- BRONDYK, W., "Selecting an appropriate method for expressing a recombinant protein", BRONDYK, W., Richard Burgess, Murray Deutscher, Guide to Protein Purification, Elsevier, (20090000), pages 131 - 147, XP055889323
OPPOSITION- Cynthia L. Goodman, Galal N. El Sayed, Arthur H. Mcintosh, James J. Grasela, Brad Stiles, "Establishment and characterization of insect cell lines from 10 lepidopteran species", In Vitro Cell Dev Biol, (20010601), vol. 37, no. 6, pages 367 - 373, XP055889316
OPPOSITION- GRACE, "ESTABLISHMENT OF FOUR STRAINS OF CELLS FROM INSECT TISSUES GROWN IN VITRO", Nature, (19620000), vol. 195, pages 788 - 789, XP055891902
OPPOSITION- "Helicoverpa_zea", Wikipedia, URL: https://web.archive.org/web/ 20120425 101623/ http://en.wikipedia.org/wiki/ Helicoverpa_zea, XP055892074
OPPOSITION- Joseph F. Morgan, Et Al.,, "Nutrition of Animal Cells in Tissue Culture. I. Initial Studies on a Synthetic Medium (17557)", Proceedings of the Society for Experimental Biology and Medicine, (19500101), vol. 73, no. 1, pages 1 - 8, XP055254255
OPPOSITION- KOVAL et al., "pH DEPENDENCY OF CELL ATTACHMENT AND GROWTH AT BOTH CLONAL AND SUBCULTURE DENSITIES OF CULTURED LEPIDOPTERAN CELLS", In Vitro Cell . Dev. Biol., (19900000), vol. 26, no. 7, pages 665 - 670, XP008180965
OPPOSITION- KOVAL ET AL., "PH Dependency of Cell Attachment and Growth at Both Clonal and Subculture Densities of Cultured Lepidopteran Cells.", IN VITRO CELLULAR AND DEVELOPMENTAL BIOLOGY., THE ASSOCIATION, GAITHERSBURG, MD., US, US , (19900701), vol. 26, no. 7, ISSN 0883-8364, pages 665 - 670, XP008180965
OPPOSITION- KURTTI et al., "THE EFFECT OF OSMOTIC PRESSURE AND pH ON THE GROWTH OF HELIOTHIS ZEA CELLS IN VITRO", Insect and mite nutrition, (19720000), XP055892033
OPPOSITION- Murhammer, D., "Pluronic Polyols—Cell Protection", Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology, (20091215), Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology, URL: https://onlinelibrary.wiley.com/doi/10.1002/9780470054581.eib488, (20220209), XP055889320
OPPOSITION- "Precipitation by selective Denaturation", SCOPES R K, Protein Purification: Principles and Practice (Third Edition), Springer, (19940101), pages 95 - 101, XP002904861
OPPOSITION- SCHLAEGER, "Medium design for insect cell culture", Cytotechnology, (19960000), vol. 20, pages 57 - 70, XP055891982
OPPOSITION- SOHI, S., "The effect of pH and osmotic pressure on the growth and survival of three lepidopteran cell lines", SOHI, S., Kurstak; Edouard, Invertebrate systems in vitro, Elsevier, NL, (19800000), XP009191709
OPPOSITION- S.S. Sohi, "The effect of pH and osmotic pressure on the growth and survival of three lepidopteran cell lines.", S.S. Sohi, Kurstak, Edouard [Hrsg.], Invertebrate systems in vitro, NL , Elsevier , (19800101), pages 35 - 43, ISBN 0-444-80181-2, XP009191709
OPPOSITION- TNM-FH Insect Media, URL: https://www.sigmaaldrich.com/deepweb/assets/ sigmaaldrich/product/documents/112/696/t1032for.pdf
OPPOSITION- Van Der Pol Leo A., "Sparging - shear sensitivity of animal cells", Thesis, Wageningen University, Wageningen University, (19980101), Thesis, URL: https://edepot.wur.nl/198209, (20220208), XP055888795
OPPOSITION- WEISS, S et al., "Chapter 4 Insect Cell Culture in Serum-Free Media", Methods in Molecular Biology, Humana Press, (19950000), vol. 39, pages 79 - 95, XP055889336
OPPOSITION- Mcintosh A.H., Grasela J.J., Goodman C.L., "Replication of Helicoverpa zea Nuclear Polyhedrosis Virus in Homologous Cell Lines Grown in Serum-Free Media", JOURNAL OF INVERTEBRATE PATHOLOGY, SAN DIEGO, CA, US, US , (19950901), vol. 66, no. 2, doi:10.1006/jipa.1995.1073, ISSN 0022-2011, pages 121 - 124, XP055889310
OPPOSITION- Bawden Alison L, Gordon Karl H.J, Hanzlik Terry N, "The Specificity of Helicoverpa armigera Stunt Virus Infectivity", JOURNAL OF INVERTEBRATE PATHOLOGY, SAN DIEGO, CA, US, US , (19990901), vol. 74, no. 2, doi:10.1006/jipa.1999.4858, ISSN 0022-2011, pages 156 - 163, XP055889341
OPPOSITION- Goodman Cynthia L., Mcintosh Arthur H., El Sayed Galal N., Grasela James J., Stiles Brad, "Production of selected baculoviruses in newly established lepidopteran cell lines", IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY. ANIMAL., Springer US, New York, New York , (20010601), vol. 37, no. 6, doi:10.1007/BF02577574, ISSN 1071-2690, pages 374 - 379, XP055889338
OPPOSITION- IKONOMOU L, SCHNEIDER Y-J, AGATHOS S N, "Insect cell culture for industrial production of recombinant proteins.", Applied Microbiology and Biotechnology, Springer Berlin Heidelberg, Berlin/Heidelberg, Berlin/Heidelberg, (20030701), vol. 62, no. 1, doi:10.1007/s00253-003-1223-9, ISSN 0175-7598, pages 1 - 20, XP002550783
OPPOSITION- Yan-lei Wu; Lei Jiang; Yoshifumi Hashimoto; Robert R. Granados; Guo-xun Li, "Establishment, growth kinetics, and susceptibility to AcMNPV of heat tolerant lepidopteran cell lines", Virologica Sinica, SP Wuhan Institute of Virology, CAS, Heidelberg, Heidelberg , (20110612), vol. 26, no. 3, doi:10.1007/s12250-011-3177-x, ISSN 1995-820X, pages 198 - 205, XP019915201
OPPOSITION- Hirumi, H. ; Maromorosch, K., "The in vitro cultivation of embryonic leafhopper tissues", Experimental Cell Research, ELSEVIER, AMSTERDAM, NL, AMSTERDAM, NL , (19641201), vol. 36, no. 3, doi:10.1016/0014-4827(64)90318-0, ISSN 0014-4827, pages 625 - 631, XP024855822
OPPOSITION- Goldstein, N.I.; McIntosh, A.H., "The proteins and glycoproteins of insect cells in culture", INSECT BIOCHEMISTRY, LONDON, GB, GB , (19800101), vol. 10, no. 4, doi:10.1016/0020-1790(80)90013-X, ISSN 0020-1790, pages 419 - 427, XP023400808
OPPOSITION- MacConaill, M., "Calcium precipitation from mammalian physiological salines (ringer solutions) and the preparation of high [ca] media", JOURNAL OF PHARMACOLOGICAL METHODS., ELSEVIER SCIENCE PUBLISHING, NEW YORK, NY., US, US , (19850901), vol. 14, no. 2, doi:10.1016/0160-5402(85)90052-X, ISSN 0160-5402, pages 147 - 155, XP023772282
OPPOSITION- MITSUHASHI, J., "Media for Insect Cell Culture", Advances in Cell Culture, (19820000), vol. 2, pages 133 - 196, XP055888779
OPPOSITION- Cox, M.M.J. ; Hollister, J.R., "FluBlok, a next generation influenza vaccine manufactured in insect cells", BIOLOGICALS., ACADEMIC PRESS LTD., LONDON., GB, GB , (20090601), vol. 37, no. 3, doi:10.1016/j.biologicals.2009.02.014, ISSN 1045-1056, pages 182 - 189, XP026136313
OPPOSITION- Jean-Christophe Drugmand, Yves-Jacques Schneider, Spiros N. Agathos, "Insect cells as factories for biomanufacturing", BIOTECHNOLOGY ADVANCES., ELSEVIER PUBLISHING, BARKING., GB, GB , (20120901), vol. 30, no. 5, doi:10.1016/j.biotechadv.2011.09.014, ISSN 0734-9750, pages 1140 - 1157, XP055302952
OPPOSITION- Linda H.L. Lua ; Steven Reid, "Growth, viral production and metabolism of a Helicoverpa zea cell line in serum-free culture", Cytotechnology, Kluwer Academic Publishers, Do, Do , (20030701), vol. 42, no. 3, doi:10.1023/B:CYTO.0000015795.46813.44, ISSN 1573-0778, pages 109 - 120, XP019236794
OPPOSITION- Ephrussi Boris, Beadle G. W., "A Technique of Transplantation for Drosophila", American Naturalist, American Society of Naturalists, Chicago, Chicago , (19360501), vol. 70, no. 728, doi:10.1086/280658, ISSN 0003-0147, pages 218 - 225, XP055889346
OPPOSITION- G M Lee, J M Savinell, B O Palsson, "Serum Can Act as a Shear Protecting Agent in Agitated Hybridoma Cell Cultures", Hybridoma, Mary Ann Liebert, Inc., Publishers, United States, United States , doi:10.1089/hyb.1989.8.639, (19891201), pages 639 - 645, Hybridoma, URL: https://www.liebertpub.com/doi/pdf/10.1089/hyb.1989.8.639, XP055492831
OPPOSITION- Loeb Marcia J., Schneiderman Howard A., "Prolonged Survival of Insect Tissues in Vitro", ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA, ENTOMOLOGICAL SOCIETY OF AMERICA, COLLEGE PARK, MD, US, US , (19560901), vol. 49, no. 5, doi:10.1093/aesa/49.5.493, ISSN 0013-8746, pages 493 - 494, XP055889343
OPPOSITION- HSU S H; LI S Y; CROSS J H, "A CELL LINE DERIVED FROM OVARIAN TISSUE OF CULEX-TRITAENIORHYNCHUS-SUMMOROSUS", JOURNAL OF MEDICAL ENTOMOLOGY., ENTOMOLOGICAL SOCIETY OF AMERICA, LANHAM, MD., US, US , (19720101), vol. 9, no. 1, doi:10.1093/jmedent/9.1.86, ISSN 0022-2585, pages 86 - 91, XP009195277
OPPOSITION- Melnick Joseph L., "TISSUE CULTURE TECHNIQUES AND THEIR APPLICATION TO ORIGINAL ISOLATION, GROWTH, AND ASSAY OF POLIOMYELITIS AND ORPHAN VIRUSES", Annals of the New York Academy of Sciences, New York Academy of Sciences., US, US , (19550901), vol. 61, no. 4, doi:10.1111/j.1749-6632.1955.tb42532.x, ISSN 0077-8923, pages 754 - 773, XP055889315
OPPOSITION- KUO et al., "Cell-based analysis of Chikungunya virus E1protein in membrane fusion", J. Biomed. Sci., (20120000), vol. 19, no. 44, pages 1 - 12, XP055280716
OPPOSITION- Szu-Cheng Kuo;Ying-Ju Chen;Yu-Ming Wang;Pei-Yi Tsui;Ming-Der Kuo;Tzong-Yuan Wu;Szecheng J Lo, "Cell-based analysis of Chikungunya virus E1 protein in membrane fusion", Journal of Biomedical Science, Kluwer Academic Publishers, Do, Do , (20120421), vol. 19, no. 1, doi:10.1186/1423-0127-19-44, ISSN 1423-0127, page 44, XP021094766
OPPOSITION- Stefan W Metz;Corinne Geertsema;Byron E Martina;Paulina Andrade;Jacco G Heldens;Monique M van Oers;Rob W Goldbach;Just M Vlak;Gorben P Pijlman, "Functional processing and secretion of Chikungunya virus E1 and E2 glycoproteins in insect cells", VIROLOGY JOURNAL, BIOMED CENTRAL, LONDON, GB, GB , (20110715), vol. 8, no. 1, doi:10.1186/1743-422X-8-353, ISSN 1743-422X, page 353, XP021105754
OPPOSITION- GERBAL MURIEL ET AL, "Adaptation of an insect cell line of Spodoptera frugiperda to grow at 37degree C: Characterization of an endodiploid clone.", IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY. ANIMAL., Springer US, New York, New York , (20000201), vol. 36, no. 2, doi:10.1290/1071-2690(2000)036<0117:AOAICL>2.3.CO;2, ISSN 1071-2690, pages 117 - 124, XP002152389
OPPOSITION- Grasela James J., Mcintosh Arthur H., Goodman Cynthia L., Wilson Louise E., King Linda A., "Expression of the Green Fluorescent Protein Carried by Autographa californica Multiple Nucleopolyhedrovirus in Insect Cell Lines", In Vitro Cellular & Developmental Biology - Animal, Springer-Verlag, New York, New York , (20000301), vol. 36, no. 3, doi:10.1290/1071-2690(2000)036<0205:EOTGFP>2.0.CO;2, ISSN 1543-706X, pages 205 - 210, XP055888774
OPPOSITION- IKONOMOU L, ET AL., "DESIGN OF AN EFFICIENT MEDIUM FOR INSECT CELL GROWTH AND RECOMBINANT PROTEIN PRODUCTION", IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY. ANIMAL., Springer US, New York, New York , (20011001), vol. 37, no. 09, doi:10.1290/1071-2690(2001)037<0549:DOAEMF>2.0.CO;2, ISSN 1071-2690, pages 549 - 559, XP009058879
OPPOSITION- Goodman Cynthia L., Wang Amy A., Nabli Henda, Mcintosh Arthur H., Wittmeyer Jennifer L., Grasela James J., "Development and partial characterization of heliothine cell lines from embryonic and differentiated tissues", In Vitro Cellular & Developmental Biology - Animal, Springer-Verlag, New York, New York , (20040101), vol. 40, no. 3, doi:10.1290/1543-706X(2004)040<0089:DAPCOH>2.0.CO;2, ISSN 1543-706X, page 89, XP055888789
OPPOSITION- Mcintosh Arthur H., Ignoffo Carlo M., "Characterization of Five Cell Lines Established from Species of Heliothis", APPLIED ENTOMOLOGY AND ZOOLOGY., NIPPON OYO DOBUTSU KONCHU GAKKAI, TOKYO., JP, JP , (19830101), vol. 18, no. 2, doi:10.1303/aez.18.262, ISSN 0003-6862, pages 262 - 269, XP055889340
OPPOSITION- Stefan W. Metz, Joy Gardner, Corinne Geertsema, Thuy T. Le, Lucas Goh, Just M. Vlak, Andreas Suhrbier, Gorben P. Pijlman, "Effective Chikungunya Virus-like Particle Vaccine Produced in Insect Cells", PLoS Neglected Tropical Diseases, vol. 7, no. 3, doi:10.1371/journal.pntd.0002124, page e2124, XP055280724
SEARCH- SOHI S S ED - KURSTAK ET AL, The effect of pH and osmotic pressure on the growth and survival of three lepidopteran cell lines, INVERTEBRATE SYSTEMS IN VITRO, ELSEVIER, NL, PAGE(S) 35 - 43, (19800101), ISBN 0-444-80181-2, XP009191709 [X] 10,14-17 * the whole document * * page 35, paragraph 2 *
SEARCH- T D C Grace, "TNM-FH Insect Media", Nature Nature In Vitro, (19620101), pages 788 - 789, URL: https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Formulation/t1032for.pdf, XP055303100 [A] 1-20
SEARCH- COX M M J ET AL, "FluBlok, a next generation influenza vaccine manufactured in insect cells", BIOLOGICALS, ACADEMIC PRESS LTD., LONDON, GB, vol. 37, no. 3, doi:10.1016/J.BIOLOGICALS.2009.02.014, ISSN 1045-1056, (20090601), pages 182 - 189, (20090317), XP026136313 [X] 10-13,19-20 * the whole document * * page 183, paragraph L *
SEARCH- JEAN-CHRISTOPHE DRUGMAND ET AL, "Insect cells as factories for biomanufacturing", BIOTECHNOLOGY ADVANCES., GB, (20120901), vol. 30, no. 5, doi:10.1016/j.biotechadv.2011.09.014, ISSN 0734-9750, pages 1140 - 1157, XP055302952 [DA] 1-20 * the whole document * * section � 3.2; page 1147 *

Download Citation Report

Get a free citation report including examiner, opposition, and international search citations.

Get Citation Report

Dossier Documents

The dossier documents provide a comprehensive record of the patent’s prosecution history - including filings, correspondence, and decisions made by patent offices - and are crucial for understanding the patent’s legal journey and any challenges it may have faced during examination.

  • Date

    Description

  • Get instant alerts for new documents