| EXAMINATION | - LI P ET AL, "Biallelic knockout of the alpha-1,3 galactosyltransferase gene in porcine liver-derived cells using zinc finger nucleases", JOURNAL OF SURGICAL RESEARCH, ACADEMIC PRESS INC., SAN DIEGO, CA, US, vol. 181, no. 1, doi:10.1016/J.JSS.2012.06.035, ISSN 0022-4804, (20120703), pages E39 - E45, (20120703), XP002717543 |
| OPPOSITION | - Anonymous, "CRISPR-associated protein Cas9/Csn1 [Staphylococcus aureus subsp. aureus]", Protein, (20120907), Database accession no. CCK74173, XP055514442 |
| OPPOSITION | - Anonymous, "Lipofectamine", Wikipedia, (20230408), pages 1 - 2, Wikipedia, URL: https://en.wikipedia.org/w/index.php?title=Lipofectamine&oldid=1148853655, (20240304), XP093137098 |
| OPPOSITION | - Anonymous, "pGL3 Luciferase Reporter Vectors - Technical Manual (INSTRUCTIONS FOR USE OF PRODUCTS E1741, E1751, E1761 AND E1771)", Promega, (20070901), Promega, (20240312), XP093140419 |
| OPPOSITION | - Anonymous , "Staphylococcus aureus subsp. aureus ORFX gene and pseudo SCCmec-SCC-SCCCCRISPR element, strain M06/0171 ", Nucleotide, (20130103), Database accession no. HE980450, URL: NCBI, XP093109772 |
| OPPOSITION | - Anonymous, "Staphylococcus aureus subsp. aureus ORFX gene and pseudo SCCmec-SCC-SCcrispr element strain m06/0171", Nucleotide, (20130103), Database accession no. HE980450, URL: ncbi, XP055338801 |
| OPPOSITION | - Anonymous, "Tekmira Pharmaceuticals Completes ApoB SNALP Phase 1 Clinical Trial ", Arbutus Biopharma, (20100107), pages 1 - 3, Arbutus Biopharma, URL: https://investor.arbutusbio.com/static-files/35363e85-5784-44d2-b317-2c4911091967, (20221128), XP093002538 |
| OPPOSITION | - Bruce Alberts ; Alexander Johnson ; Julian Lewis, "Chapter 24: Pathogens, Infection, and Innate Immunity", Bruce Alberts ; Alexander Johnson ; Julian Lewis, ALBERTS B. ET AL., MOLECULAR BIOLOGY OF THE CELL, NEW YORK , GARLAND SCIENCE , (20080101), pages 1485 - 1538, ISBN 978-0-8153-4105-5, XP009554138 |
| OPPOSITION | - CONG LE ET AL, "Multiplex Genome Engineering Using CRISPR/Cas Systems", Science, American Association for the Advancement of Science, US, US , (20130201), vol. 339, no. 6121, ISSN 0036-8075, pages 819 - 823, XP002779503 |
| OPPOSITION | - Coura Renata dos Santos; Nardi Nance Beyer, "The state of the art of adeno-associated virus-based vectors in gene therapy", VIROLOGY JOURNAL, BIOMED CENTRAL, LONDON, GB, GB , (20071016), vol. 4, no. 1, ISSN 1743-422X, page 99, XP021038308 |
| OPPOSITION | - FIECK ET AL, "Modifications of the E. coli Lac repressor for expression in eukaryotic cells: effects of nuclear signal sequences on protein activity and nuclear acumulation", Nucleic Acids Research, Oxford University Press, GB, GB , (19920101), vol. 20., no. 07., ISSN 0305-1048, pages 1785 - 1791., XP002095852 |
| OPPOSITION | - Laura Schramm, Hernandez Nouria, "REVIEW Recruitment of RNA polymerase III to its target promoters", Genes & Development, Cold Spring Harbor Laboratory Press, vol. 16, (20020101), pages 2593 - 2620, Genes & Development, URL: http://genesdev.cshlp.org/content/16/20/2593.full.pdf, (20150416), XP055183585 |
| OPPOSITION | - MCLEAN et al., "Gene targeted therapeutics for liver disease in alpha-1 antitrypsin deficiency", Biologics: Targets & Therapy, (20090000), vol. 3, pages 63 - 75, XP055140952 |
| OPPOSITION | - Natália Freitas, Cunha Celso, "Mechanisms and Signals for the Nuclear Import of Proteins", Current Genomics, (20090101), vol. 10, no. 29, pages 550 - 557, XP055502464 |
| OPPOSITION | - Press release: Tekmira Pharmaceuticals, (20090403), URL: https://www.pharmaceuticalonline.com/doc/tekmiras-partner-alnylam-initiates-dosing-in-0001, XP055937949 |
| OPPOSITION | - Richard R. Sinden, "Chapter 3: DNA Supercoiling", Richard R. Sinden, Richard R. Sinden, DNA Structure and Function, Academic Press, (19940101), pages 95 - 133, ISBN 0-12-645750-6, XP009554135 |
| OPPOSITION | - Richard R. Sinden, "Chapter 9: The Organization of DNA into Chromosomes", Richard R. Sinden, Richard R. Sinden, DNA Structure and Function, Academic Press, (19940101), pages 326 - 352, ISBN 0-12-645750-6, XP009554136 |
| OPPOSITION | - SEMPLE SEAN C ET AL, "Efficient encapsulation of antisense oligonucleotides in lipid vesicles using ionizable aminolipids: Formation of novel small multilamellar vesicle structures.", (20010101), vol. 1510, no. 1-2, pages 152 - 166, XP002249378 |
| OPPOSITION | - WANG et al., "Sustained correction of disease in naive andAAV2-pretreated hemophilia B dogs: AAV 2/8- mediated, liver-directed gene therapy", Blood, (20050415), vol. 105, no. 8, pages 3079 - 3086, XP055937937 |
| OPPOSITION | - Wu Jianqing, Et Al, "Self-Complementary Recombinant Adeno- Associated Viral Vectors: Packaging Capacity And The Role of Rep Proteins in Vector Purity", Human Gene Therapy, (20070201), vol. 18, no. 3, pages 171 - 182, XP055937964 |
| OPPOSITION | - Muthusamy Jayaraman; Steven M. Ansell; Barbara L. Mui; Ying K. Tam; Jianxin Chen; Xinyao Du; David Butler; Laxman Eltepu; Shigeo Matsuda; Jayaprakash K. Narayanannair; Kallanthottathil G. Rajeev; Ismail M. Hafez; Akin Akinc; Martin A. Maier; Mark A. Tracy; Pieter R. Cullis; Thomas D. Madden; Muthiah Manoharan; Michael J. Hope, "Maximizing the Potency of siRNA Lipid Nanoparticles for Hepatic Gene Silencing In Vivo", Angewandte Chemie International Edition, Verlag Chemie, Hoboken, USA, Hoboken, USA, (20120710), vol. 51, no. 34, doi:10.1002/anie.201203263, ISSN 1433-7851, pages 8529 - 8533, XP072070912 |
| OPPOSITION | - Sandra Cristea, Freyvert, Santiago, Holes, Urnov, Gregory, Cost, "In vivo cleavage of transgene donors promotes nuclease-mediated targeted integration", BIOTECHNOLOGY AND BIOENGINEERING, (20130301), vol. 110, no. 3, doi:10.1002/bit.24733, pages 871 - 880, XP055076901 |
| OPPOSITION | - Céline Souilhol, Sarah Cormier, Marie Monet, Sandrine Vandormael-Pournin, Anne Joutel, Charles Babinet, Michel Cohen-Tannoudji, "Nas transgenic mouse line allows visualization of Notch pathway activity in vivo", genesis, Wiley-Liss, (20060601), vol. 44, no. 6, doi:10.1002/dvg.20208, ISSN 1526-954X, pages 277 - 286, XP055502571 |
| OPPOSITION | - Daisuke Furutama, Morita Noriyuki, Takano Riya, Sekine Yukiko, Sadakata Tetsushi, Shinoda Yo, Hayashi Kanehiro, Mishima Yuriko, Mikoshiba Katsuhiko, Hawkes Richard, Furuichi Teiichi, "Expression of the IP3R1 promoter-driven nls-lacZ transgene in Purkinje cell parasagittal arrays of developing mouse cerebellum", Journal of Neuroscience Research, WILEY-LISS, US, US , (20100101), vol. 88, doi:10.1002/jnr.22451, ISSN 0360-4012, pages 2810 - 2825, XP055502574 |
| OPPOSITION | - Ranieri Bizzarri; Francesco Cardarelli; Michela Serresi; Fabio Beltram, "Fluorescence recovery after photobleaching reveals the biochemistry of nucleocytoplasmic exchange", Analytical and Bioanalytical Chemistry, Springer, Berlin, DE, Berlin, DE , (20120515), vol. 403, no. 8, doi:10.1007/s00216-012-6025-4, ISSN 1618-2650, pages 2339 - 2351, XP035069015 |
| OPPOSITION | - PATTERSON STACEY S ET AL, "Codon optimization of bacterial luciferase (lux) for expression in mammalian cells", Journal of Industrial Microbiology & Biotechnology, BASINGSTOKE, GB, GB , (20050301), vol. 32, no. 3, doi:10.1007/s10295-005-0211-8, ISSN 1367-5435, pages 115 - 123, XP002503994 |
| OPPOSITION | - Rémy Séverine, Tesson Laurent, Ménoret Séverine, Usal Claire, Scharenberg Andrew M., Anegon Ignacio, "Zinc-finger nucleases: a powerful tool for genetic engineering of animals", Transgenic Research, Kluwer Academic Publishers-Plenum Publishers, Ne, Ne , (20100601), vol. 19, no. 3, doi:10.1007/s11248-009-9323-7, ISSN 1573-9368, pages 363 - 371, XP019831645 |
| OPPOSITION | - Erickson Harold P, "Size and Shape of Protein Molecules at the Nanometer Level Determined by Sedimentation, Gel Filtration, and Electron Microscopy", BIOLOGICAL PROCEDURES ONLINE, BIOLOGICAL PROCEDURES ONLINE, WATERLOO, CA, CA , (20090515), vol. 11, no. 1, doi:10.1007/s12575-009-9008-x, ISSN 1480-9222, page 9008, XP021088900 |
| OPPOSITION | - Dingwall, C. ; Sharnick, S.V. ; Laskey, R.A., "A polypeptide domain that specifies migration of nucleoplasmin into the nucleus", Cell, Elsevier, Amsterdam NL, Amsterdam NL , (19820901), vol. 30, no. 2, doi:10.1016/0092-8674(82)90242-2, ISSN 0092-8674, pages 449 - 458, XP023875960 |
| OPPOSITION | - KALDERON D, ET AL., "A SHORT AMINO ACID SEQUENCE ABLE TO SPECIFY NUCLEAR LOCATION", Cell, Elsevier, Amsterdam NL, Amsterdam NL , (19841201), vol. 39, no. 03, doi:10.1016/0092-8674(84)90457-4, ISSN 0092-8674, pages 499 - 509, XP008022368 |
| OPPOSITION | - ROBERTS B L, RICHARDSON W D, SMITH A E, "THE EFFECT OF PROTEIN CONTEXT ON NUCLEAR LOCATION SIGNAL FUNCTION", Cell, Elsevier, Amsterdam NL, Amsterdam NL , (19870731), vol. 50, no. 03, doi:10.1016/0092-8674(87)90500-9, ISSN 0092-8674, pages 465 - 475, XP001194545 |
| OPPOSITION | - FOECKING M K, HOFSTETTER H, "Powerful and versatile enhancer-promoter unit for mammalian expression vectors", Gene, Elsevier Amsterdam, NL, NL , (19860101), vol. 45, no. 01, doi:10.1016/0378-1119(86)90137-X, ISSN 0378-1119, pages 101 - 105, XP009061501 |
| OPPOSITION | - Scott A. Barros, Gollob Jared A., "Safety profile of RNAi nanomedicines", Advanced Drug Delivery Reviews, Elsevier, (20121201), vol. 64, no. 15, doi:10.1016/j.addr.2012.06.007, ISSN 0169409X, pages 1730 - 1737, XP055078049 |
| OPPOSITION | - OZCAN et al., "Preclinical and clinical development of siRNA-based therapeutics", Adv. Drug Deliv. Rev., (20150629), vol. 87, doi:10.1016/j.addr.2015.01.007, pages 108 - 119, XP055504648 |
| OPPOSITION | - Pablo Perez-Pinera, David G Ousterout, Charles A Gersbach, "Advances in targeted genome editing", Current Opinion in Chemical Biology, Elsevier Current Trends, (20120801), vol. 16, no. 3-4, doi:10.1016/j.cbpa.2012.06.007, ISSN 13675931, pages 268 - 277, XP055065262 |
| OPPOSITION | - Lei s. Qi, Matthew h. Larson, Luke a. Gilbert, Jennifer a. Doudna, Jonathan s. Weissman, Adam p. Arkin, Wendell a. Lim, "Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression", Cell, Cell Press, (20130201), vol. 152, no. 5, doi:10.1016/j.cell.2013.02.022, ISSN 00928674, pages 1173 - 1183, XP055068548 |
| OPPOSITION | - Wang Haoyi; Yang Hui; Shivalila Chikdu S.; Dawlaty Meelad M.; Cheng Albert W.; Zhang Feng; Jaenisch Rudolf, "One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering", Cell, Elsevier, Amsterdam NL, Amsterdam NL , (20130509), vol. 153, no. 4, doi:10.1016/j.cell.2013.04.025, ISSN 0092-8674, pages 910 - 918, XP028538358 |
| OPPOSITION | - Luke et al, "CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes (includes Supplemental Information)", Cell, Elsevier, Amsterdam NL, Amsterdam NL , (20130718), vol. 154, no. 2, doi:10.1016/j.cell.2013.06.044, ISSN 0092-8674, pages 442 - 451,S1-S5, XP055321615 |
| OPPOSITION | - Yang Hui; Wang Haoyi; Shivalila Chikdu S.; Cheng Albert W.; Shi Linyu; Jaenisch Rudolf, "One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated Genome Engineering", Cell, Elsevier, Amsterdam NL, Amsterdam NL , (20130912), vol. 154, no. 6, doi:10.1016/j.cell.2013.08.022, ISSN 0092-8674, pages 1370 - 1379, XP028716273 |
| OPPOSITION | - Andrew r. Bassett, Charlotte Tibbit, Chris p. Ponting, Ji-Long Liu, "Highly Efficient Targeted Mutagenesis of Drosophila with the CRISPR/Cas9 System", Cell Reports, Elsevier Inc, US, US , (20130701), vol. 4, no. 1, doi:10.1016/j.celrep.2013.06.020, ISSN 2211-1247, pages 220 - 228, XP055237308 |
| OPPOSITION | - Jonathan D. Finn, Amy Rhoden Smith, Mihir C. Patel, Lucinda Shaw, Madeleine R. Youniss, Jane Van Heteren, Tanner Dirstine, Corey Ciullo, Reynald Lescarbeau, Jessica Seitzer, Ruchi R. Shah, Aalok Shah, Dandan Ling, Jacqueline Growe, Melissa Pink, Ellen Rohde, Kristy M. Wood, William E. Salomon, William F. Harrington, Christian Dombrowski, Walter R. Strapps, Yong Chang, David V. Morrissey, "A Single Administration of CRISPR/Cas9 Lipid Nanoparticles Achieves Robust and Persistent In Vivo Genome Editing", Cell Reports, Elsevier Inc, US, US , (20180201), vol. 22, no. 9, doi:10.1016/j.celrep.2018.02.014, ISSN 2211-1247, pages 2227 - 2235, XP055527484 |
| OPPOSITION | - Claudio Mussolino, Toni Cathomen, "TALE nucleases: tailored genome engineering made easy", Current Opinion in Biotechnology, Elsevier Current Trends, (20121001), vol. 23, no. 5, doi:10.1016/j.copbio.2012.01.013, ISSN 09581669, pages 644 - 650, XP055071668 |
| OPPOSITION | - Do, H.J. Song, H. Yang, H.M. Kim, D.K. Kim, N.H. Kim, J.H. Cha, K.Y. Chung, H.M. Kim, J.H., "Identification of multiple nuclear localization signals in murine Elf3, an ETS transcription factor", FEBS Letters, ELSEVIER, AMSTERDAM., NL, NL , (20060320), vol. 580, no. 7, doi:10.1016/j.febslet.2006.02.049, ISSN 0014-5793, pages 1865 - 1871, XP005335679 |
| OPPOSITION | - YAN et al., "Human thyroxine binding globulin (TBG) promoter directs efficient and sustaining transgene expression in liver-specific pattern", Gene, (20120720), vol. 506, doi:10.1016/j.gene.2012.07.009, pages 289 - 294, XP055350459 |
| OPPOSITION | - Li P, Estrada J L, Burlak C, Tector A J, "Biallelic knockout of the alpha-1,3 galactosyltransferase gene in porcine liver-derived cells using zinc finger nucleases", Journal of Surgical Research, ACADEMIC PRESS INC., SAN DIEGO, CA., US, US , (20130501), vol. 181, no. 1, doi:10.1016/j.jss.2012.06.035, ISSN 0022-4804, pages E39 - E45, XP002717543 |
| OPPOSITION | - Yuxuan Wu, Dan Liang, Yinghua Wang, Meizhu Bai, Wei Tang, Shiming Bao, Zhiqiang Yan, Dangsheng Li, Jinsong Li, "Correction of a Genetic Disease in Mouse via Use of CRISPR-Cas9", Cell Stem Cell, Elsevier, Cell Press, (20131201), vol. 13, no. 6, doi:10.1016/j.stem.2013.10.016, ISSN 19345909, pages 659 - 662, XP055196555 |
| OPPOSITION | - Gerald Schwank, Bon-Kyoung Koo, Valentina Sasselli, Johanna f. Dekkers, Inha Heo, Turan Demircan, Nobuo Sasaki, Sander Boymans, Edwin Cuppen, Cornelis k. Van der ent, Edward e.S. Nieuwenhuis, Jeffrey m. Beekman, Hans Clevers, "Functional Repair of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients", Cell Stem Cell, Elsevier, Cell Press, (20131201), vol. 13, no. 6, doi:10.1016/j.stem.2013.11.002, ISSN 19345909, pages 653 - 658, XP055102687 |
| OPPOSITION | - Goldfarb, D.S. Corbett, A.H. Mason, D.A. Harreman, M.T. Adam, S.A., "Importin α: a multipurpose nuclear-transport receptor", Trends in cell biology, ELSEVIER SCIENCE LTD., XX, XX , (20040901), vol. 14, no. 9, doi:10.1016/j.tcb.2004.07.016, ISSN 0962-8924, pages 505 - 514, XP004552656 |
| OPPOSITION | - Thomas Gaj, Charles A. Gersbach, Carlos F. Barbas, "ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering", TRENDS IN BIOTECHNOLOGY., ELSEVIER PUBLICATIONS, CAMBRIDGE., GB, GB , (20130701), vol. 31, no. 7, doi:10.1016/j.tibtech.2013.04.004, ISSN 0167-7799, pages 397 - 405, XP055418607 |
| OPPOSITION | - Daniel Boden, Et Al, "Efficient Gene Transfer of HIV-1-Specific Short Hairpin RNA into Human Lymphocytic Cells Using Recombinant Adeno-associated Virus Vectors", Molecular Therapy, Elsevier Inc., US, US , (20040301), vol. 9, no. 3, doi:10.1016/j.ymthe.2003.11.025, ISSN 1525-0016, pages 396 - 402, XP055487100 |
| OPPOSITION | - Judge, A.D. Bola, G. Lee, A.C.H. MacLachlan, I., "Design of Noninflammatory Synthetic siRNA Mediating Potent Gene Silencing in Vivo", Molecular Therapy, Elsevier Inc., US, US , (20060301), vol. 13, no. 3, doi:10.1016/j.ymthe.2005.11.002, ISSN 1525-0016, pages 494 - 505, XP005326762 |
| OPPOSITION | - Imagawa M. Sakaue R. Tanabe A. Osada S. Nishihara T., "Two nuclear localization signals are required for nuclear translocation of nuclear factor 1-A", FEBS Letters, ELSEVIER, AMSTERDAM., NL, NL , (20001103), vol. 484, no. 2, doi:10.1016/S0014-5793(00)02119-0, ISSN 0014-5793, pages 118 - 124, XP004337724 |
| OPPOSITION | - Van den Ackerveken Guido; Marois Eric; Bonas Ulla, "Recognition of the Bacterial Avirulence Protein AvrBs3 Occurs inside the Host Plant Cell", Cell, Elsevier, Amsterdam NL, Amsterdam NL , (19951227), vol. 87, no. 7, doi:10.1016/S0092-8674(00)81825-5, ISSN 0092-8674, pages 1307 - 1316, XP029186424 |
| OPPOSITION | - GEISBERT THOMAS W ET AL, "Postexposure protection of non-human primates against a lethal Ebola virus challenge with RNA interference: a proof-of-concept study.", The Lancet, ELSEVIER, AMSTERDAM, NL, AMSTERDAM, NL , (20100529), vol. 375, no. 9729, doi:10.1016/S0140-6736(10)60357-1, ISSN 0140-6736, pages 1896 - 1905, XP002610586 |
| OPPOSITION | - KARRAN P, "DNA DOUBLE STRAND BREAK REPAIR IN MAMMALIAN CELLS", CURRENT OPINION IN GENETICS & DEVELOPMENT., CURRENT BIOLOGY LTD., XX, XX , (20000401), vol. 10, no. 02, doi:10.1016/S0959-437X(00)00069-1, ISSN 0959-437X, pages 144 - 150, XP001206008 |
| OPPOSITION | - Manuela Villion, Sylvain Moineau, "The double-edged sword of CRISPR-Cas systems", Cell Research, (20130101), vol. 23, no. 1, doi:10.1038/cr.2012.124, ISSN 10010602, pages 15 - 17, XP055086684 |
| OPPOSITION | - CHENG ALBERT W, ET AL, "Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system", Cell Research, Springer Singapore, Singapore, Singapore , (20131001), vol. 23, no. 10, doi:10.1038/CR.2013.122, ISSN 1001-0602, pages 1163 - 1171, XP002735688 |
| OPPOSITION | - Nannan Chang, Changhong Sun, Lu Gao, Dan Zhu, Xiufei Xu, Xiaojun Zhu, Jig-Wei Xiong, Jianzhong Jeff Xi, "Genome editing with RNA-guided Cas9 nuclease in Zebrafish embryos", Cell Research, Springer Singapore, Singapore, Singapore , (20130401), vol. 23, no. 4, doi:10.1038/cr.2013.45, ISSN 1001-0602, pages 465 - 472, XP055251146 |
| OPPOSITION | - D Carroll, "Progress and prospects: Zinc-finger nucleases as gene therapy agents", Gene Therapy, Macmillan Press Ltd., (20081101), vol. 15, no. 22, doi:10.1038/gt.2008.145, ISSN 09697128, pages 1463 - 1468, XP055041104 |
| OPPOSITION | - HIBBITT et al., "RNAi-mediated knockdown of HMG CoA reductase enhances gene expression from physiologically regulated low-density lipoprotein receptor therapeutic vectors in vivo", Gene Therapy, (20110728), vol. 19, doi:10.1038/gt.2011.103, pages 463 - 467, XP055073263 |
| OPPOSITION | - Hibbitt O; Agkatsev S; Owen C; Cioroch M; Seymour L; Channon K; Wade-Martins R, "RNAi-mediated knockdown of HMG CoA reductase enhances gene expression from physiologically regulated low-density lipoprotein receptor therapeutic vectors in vivo", Gene Therapy, Nature Publishing Group, London, GB, GB , (20110728), vol. 19, no. 4, doi:10.1038/gt.2011.103, ISSN 0969-7128, pages 463 - 467, XP037770485 |
| OPPOSITION | - Li L, Wang R, Wilcox D, Zhao X, Song J, Lin X, Kohlbrenner W M, Fesik S W, Shen Y, "Tumor vasculature is a key determinant for the efficiency of nanoparticle-mediated siRNA delivery", Gene Therapy, Nature Publishing Group, London, GB, GB , (20120701), vol. 19, no. 7, doi:10.1038/gt.2011.146, ISSN 0969-7128, pages 775 - 780, XP093002513 |
| OPPOSITION | - MCCARTY, "Self-complementary AAV Vectors; Advances and Applications", Mol Ther., (20080805), vol. 16, no. 10, doi:10.1038/mt.2008.171, pages 1648 - 1656, XP002714134 |
| OPPOSITION | - CUNNINGHAM et al., "Gene Delivery to the Juvenile Mouse Liver Using AAV2/8 Vectors", Molecular Therapy, (20080415), vol. 16, no. 6, doi:10.1038/mt.2008.72, pages 1081 - 1088, XP055610915 |
| OPPOSITION | - Zhijian Wu, Hongyan Yang, Peter Colosi, "Effect of Genome Size on AAV Vector Packaging", Molecular Therapy, Elsevier Inc., US, US , (20100101), vol. 18, no. 1, doi:10.1038/mt.2009.255, ISSN 1525-0016, pages 80 - 86, XP055235504 |
| OPPOSITION | - Annemart Koornneef, Piotr Maczuga, Richard van Logtenstein, Florie Borel, Bas Blits, Tita Ritsema, Sander van Deventer, Harald Petry, Pavlina Konstantinova, "Apolipoprotein B Knockdown by AAV-delivered shRNA Lowers Plasma Cholesterol in Mice"", Molecular Therapy, Elsevier Inc., US, US , (20110401), vol. 19, no. 4, doi:10.1038/MT.2011.6, ISSN 1525-0016, pages 731 - 740, XP002732021 |
| OPPOSITION | - NISCHAL et al., "Long-term Expression of Apolipoprotein B mRNA-specific Hammerhead Riboyzme via scAAV8. 2 Vector Inhibits Atherosclerosis in mice", Molecular Therapy - Nucleic Acids, (20131001), vol. 2, XP055937943 |
| OPPOSITION | - ZIMMERMANN TRACY S ET AL, "RNAi-mediated gene silencing in non-human primates.", Nature, Nature Publishing Group UK, London, London, (20060504), vol. 441, no. 7089, doi:10.1038/nature04688, ISSN 0028-0836, pages 111 - 114, XP002412249 |
| OPPOSITION | - Mark E. Kleinman, Kiyoshi Yamada, Atsunobu Takeda, Vasu Chandrasekaran, Miho Nozaki, Judit Z. Baffi, Romulo J. C. Albuquerque, Satoshi Yamasaki, Masahiro Itaya, Yuzhen Pan, Binoy Appukuttan, Daniel Gibbs, Zhenglin Yang, Katalin Karikó, Balamurali K. Ambati, Traci A. Wilgus, Luisa A. DiPietro, Eiji Sakurai, Kang Zhang, Justine R. Smith, Ethan W. Taylor, Jayakrishna Ambati, "Sequence- and target-independent angiogenesis suppression by siRNA via TLR3", Nature, (20080403), vol. 452, no. 7187, doi:10.1038/nature06765, pages 591 - 598, XP002650477 |
| OPPOSITION | - Elitza Deltcheva, Krzysztof Chylinski, Cynthia M. Sharma, Karine Gonzales, Yanjie Chao, Zaid A. Pirzada, Maria R. Eckert, Jörg Vogel, Emmanuelle Charpentier, "CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III", Nature, Nature Publishing Group UK, London, London, (20110331), vol. 471, no. 7340, doi:10.1038/nature09886, ISSN 0028-0836, pages 602 - 607,2pp, XP055068535 |
| OPPOSITION | - Li H, Haurigot V, Doyon Y, Li T, Wong S Y, Bhagwat A S, Malani N, Anguela X M, Sharma R, Ivanciu L, Murphy S L, Finn J D, Khazi F R, Zhou S, Paschon D E, Rebar E J, Bushman F D, Gregory P D, Holmes M C, High K A, "In vivo genome editing restores haemostasis in a mouse model of haemophilia", Nature, Nature Publishing Group UK, London, London, (20110714), vol. 475, no. 7355, doi:10.1038/nature10177, ISSN 0028-0836, pages 217 - 221, XP002688791 |
| OPPOSITION | - Yusa Kosuke; Rashid S. Tamir; Strick-Marchand Helene; Varela Ignacio; Liu Pei-Qi; Paschon David E.; Miranda Elena; Ordóñez Adriana; Hannan Nicholas R. F.; Rouhani Foad J.; Darche Sylvie; Alexander Graeme; Marciniak Stefan J.; Fusaki Noemi; Hasegawa Mamoru; Holmes Michael C.; Di Santo James P.; Lomas David A.; Bradley Allan; Vallier Ludovic, "Targeted gene correction of α1-antitrypsin deficiency in induced pluripotent stem cells", Nature, Nature Publishing Group UK, London, London, (20111012), vol. 478, no. 7369, doi:10.1038/nature10424, ISSN 0028-0836, pages 391 - 394, XP037548563 |
| OPPOSITION | - Victoria M. Bedell, Ying Wang, Jarryd M. Campbell, Tanya L. Poshusta, Colby G. Starker, Randall G. Krug Ii, Wenfang Tan, Sumedha G. Penheiter, Alvin C. Ma, Anskar Y. H. Leung, Scott C. Fahrenkrug, Daniel F. Carlson, Daniel F. Voytas, Karl J. Clark, Jeffrey J. Essner, Stephen C. Ekker, "In vivo genome editing using a high-efficiency TALEN system", Nature, ¬Macmillan Journals Ltd., etc.|, (20120101), vol. 491, no. 7422, doi:10.1038/nature11537, ISSN 00280836, pages 114 - 118, XP055048281 |
| OPPOSITION | - MORRISSEY DAVID V ET AL, "Potent and persistent in vivo anti-HBV activity of chemically modified siRNAs.", Nature Biotechnology, Nature Publishing Group US, New York, New York, (20050801), vol. 23, no. 8, doi:10.1038/nbt1122, ISSN 1087-0156, pages 1002 - 1007, XP002452540 |
| OPPOSITION | - Morrissey David V, Jennifer A Lockridge; Lucinda Shaw; Karin Blanchard; Kristi Jensen; Wendy Breen; Kimberly Hartsough; Lynn Machemer; Susan Radka; Vasant Jadhav; Narendra Vaish; Shawn Zinnen; Chandra Vargeese; Keith Bowman; Chris S Shaffer; Lloyd B Jeffs; Adam Judge; Ian Maclachlan & Barry Polisky, "Supplementary information: Potent and persistent in vivo anti-HBV activity of chemically modified siRNAs", Nature Biotechnology, Nature Publishing Group US, New York, New York, (20050801), vol. 23, no. 8, doi:10.1038/nbt1122, ISSN 1087-0156, pages 1 - 6, XP093137097 |
| OPPOSITION | - MORRISSEY et al., "Potent and persistent in vivo anti- HBV activity of chemically modified siRNAs", Nat Biotechnol., (20050724), vol. 23, no. 8, doi:10.1038/nbt1122, pages 1002 - 1007, XP008157520 |
| OPPOSITION | - WOLFF J A, SEBESTY¹N M G, "Nuclear security breached: DNA chemistry may provide a solution to the deceptively difficult problem of enhancing DNA nuclear transport in nonviral vectors", Nature Biotechnology, Nature Publishing Group US, New York, New York, (20011201), vol. 19, doi:10.1038/nbt1201-1118, ISSN 1087-0156, pages 1118 - 1120, XP002969666 |
| OPPOSITION | - LOMBARDO ANGELO, ET AL, "Gene editing in human stem cells using zinc finger nucleases and integrase-defective lentiviral vector delivery.", Nature Biotechnology, Nature Publishing Group US, New York, New York, (20071101), vol. 25, no. 11, doi:10.1038/nbt1353, ISSN 1087-0156, pages 1298 - 1306, XP002465562 |
| OPPOSITION | - Sean C Semple, Akin Akinc, Jianxin Chen, Ammen P Sandhu, Barbara L Mui, Connie K Cho, Dinah W Y Sah, Derrick Stebbing, Erin J Crosley, Ed Yaworski, et al., "Rational design of cationic lipids for siRNA delivery", Nature Biotechnology, Nature Publishing Group US, New York, New York, (20100201), vol. 28, no. 2, doi:10.1038/NBT.1602, ISSN 1087-0156, pages 172 - 176, XP002633693 |
| OPPOSITION | - Cui Xiaoxia; Ji Diana; Fisher Daniel A; Wu Yumei; Briner David M; Weinstein Edward J, "Targeted integration in rat and mouse embryos with zinc-finger nucleases", Nature Biotechnology, Nature Publishing Group US, New York, New York, (20110101), vol. 29, no. 1, doi:10.1038/nbt.1731, ISSN 1087-0156, pages 64 - 67, XP037104089 |
| OPPOSITION | - Jeffrey C Miller, Siyuan Tan, Guijuan Qiao, Kyle A Barlow, Jianbin Wang, Danny F Xia, Xiangdong Meng, David E Paschon, Elo Leung, Sarah J Hinkley, Gladys P Dulay, Kevin L Hua, Irina Ankoudinova, Gregory J Cost, Fyodor D Urnov, H Steve Zhang, Michael C Holmes, Lei Zhang, Philip D Gregory & Edward J Rebar, "A TALE nuclease architecture for efficient genome editing.(includes Online Methods)", Nature Biotechnology, (20110201), vol. 29, no. 2, doi:10.1038/nbt.1755, ISSN 1546-1696, pages 143 - 148+2pp, XP002685898 |
| OPPOSITION | - Dirk Hockemeyer, Haoyi Wang, Samira Kiani, Christine S Lai, Qing Gao, John P Cassady, Gregory J Cost, Lei Zhang, Yolanda Santiago, Jeffrey C Miller, Bryan Zeitler, Jennifer M Cherone, Xiangdong Meng, Sarah J Hinkley, Edward J Rebar, Philip D Gregory, Fyodor D Urnov, Rudolf Jaenisch, "Genetic engineering of human pluripotent cells using TALE nucleases", Nature Biotechnology, Gale Group Inc., (20110801), vol. 29, no. 8, doi:10.1038/nbt.1927, ISSN 10870156, pages 731 - 734, XP055018244 |
| OPPOSITION | - Laurent Tesson, Claire Usal, Séverine Ménoret, Elo Leung, Brett J Niles, Séverine Remy, Yolanda Santiago, Anna I Vincent, Xiangdong Meng, Lei Zhang, Philip D Gregory, Ignacio Anegon, Gregory J Cost, "Knockout rats generated by embryo microinjection of TALENs.", Nature Biotechnology, Nature Publishing Group US, New York, New York, (20110801), vol. 29, no. 8, doi:10.1038/NBT.1940, ISSN 1087-0156, pages 695 - 696, XP002681970 |
| OPPOSITION | - Rodolphe Barrangou, "RNA-mediated programmable DNA cleavage", Nature Biotechnology, Nature Publishing Group US, New York, New York, (20120910), vol. 30, no. 9, doi:10.1038/nbt.2357, ISSN 1087-0156, pages 836 - 838, XP055118675 |
| OPPOSITION | - Woong Y Hwang, Yanfang Fu, Deepak Reyon, Morgan L Maeder, Shengdar Q Tsai, Jeffry D Sander, Randall T Peterson, J-R Joanna Yeh, J Keith Joung, "Efficient genome editing in zebrafish using a CRISPR-Cas system", Nature Biotechnology, Nature Publishing Group US, New York, New York, (20130129), vol. 31, no. 3, doi:10.1038/nbt.2501, ISSN 1087-0156, pages 227 - 229, XP055086625 |
| OPPOSITION | - Seung Woo Cho, Sojung Kim, Jong Min Kim, Jin-Soo Kim, "Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease (includes Supplementary information)", Nature Biotechnology, Nature Publishing Group US, New York, New York, (20130301), vol. 31, no. 3, doi:10.1038/nbt.2507, ISSN 1087-0156, pages 230 - 232+1-11, XP002699850 |
| OPPOSITION | - Patrick D Hsu, David A Scott, Joshua A Weinstein, F Ann Ran, Silvana Konermann, Vineeta Agarwala, Yinqing Li, Eli J Fine, Xuebing Wu, Ophir Shalem, Thomas J Cradick, Luciano A Marraffini, Gang Bao & Feng Zhang, "DNA targeting specificity of RNA-guided Cas9 nucleases.", Nature Biotechnology, (20130901), vol. 31, no. 9, doi:10.1038/nbt.2647 , ISSN 1546-1696, pages 827 - 832, XP002718604 |
| OPPOSITION | - Hao Yin, Wen Xue, Sidi Chen, Roman L Bogorad, Eric Benedetti, Markus Grompe, Victor Koteliansky, Phillip A Sharp, Tyler Jacks, Daniel G Anderson, "Genome editing with Cas9 in adult mice corrects a disease mutation and phenotype", Nature Biotechnology, Gale Group Inc., vol. 32, no. 6, doi:10.1038/nbt.2884, ISSN 10870156, pages 551 - 553, XP055176822 |
| OPPOSITION | - YIN et al., "Genome editing with Cas9 in adult mice corrects a disease mutation and phenotype", Nat Biotechnol., (20140330), vol. 32, no. 6, doi:10.1038/nbt.2884, pages 551 - 553, XP055176941 |
| OPPOSITION | - John A Zuris, David B Thompson, Yilai Shu, John P Guilinger, Jeffrey L Bessen, Johnny H Hu, Morgan L Maeder, J Keith Joung, Zheng-Yi Chen, David R Liu, "Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo", Nature Biotechnology, Nature Publishing Group US, New York, New York, (20150101), vol. 33, no. 1, doi:10.1038/nbt.3081, ISSN 1087-0156, pages 73 - 80, XP055562063 |
| OPPOSITION | - REBAR ET AL, "Induction of angiogenesis in a mouse model using engineered transcription factors", Nature Medicine, Nature Publishing Group US, New York, New York, (20021104), vol. 8, no. 12, doi:10.1038/nm795, ISSN 1078-8956, pages 1427 - 1432, XP002993087 |
| OPPOSITION | - KANASTY et al., "Delivery materials for siRNA therapeutics", Nature Materials, (20131023), vol. 12, doi:10.1038/nmat3765, pages 967 - 977, XP055181421 |
| OPPOSITION | - Prashant Mali, Kevin M Esvelt & George M Church, "Cas9 as a versatile tool for engineering biology.", NATURE METHODS, Nature Publishing Group, GB, GB , (20131001), vol. 10, no. 10, doi:10.1038/nmeth.2649 , ISSN 1548-7105, pages 957 - 963, XP002718606 |
| OPPOSITION | - Kevin M Esvelt, Mali Prashant, Braff Jonathan L, Moosburner Mark, Yaung Stephanie J, Church George M, "Orthogonal Cas9 proteins for RNA-guided gene regulation and editing", Nature Methods, Nature Pub. Group, (20130929), vol. 10, no. 11, doi:10.1038/nmeth.2681, ISSN 15487091, pages 1116 - 1121, XP055128928 |
| OPPOSITION | - RAN et al., "Genome engineering using the CRISPR-Cas9 system", Nature Protocols, (20131024), vol. 8, no. 11, doi:10.1038/nprot.2013.143, pages 2281 - 2308, XP009174668 |
| OPPOSITION | - WHITEHEAD KATHRYN A , LANGER ROBERT, ANDERSON DANIEL G, "Knocking down barriers: advances in siRNA delivery.", Nature Reviews. Drug Discovery, Nature Publishing Group, GB, GB , (20090201), vol. 8, no. 2, doi:10.1038/nrd2742, ISSN 1474-1784, pages 129 - 138, XP002559384 |
| OPPOSITION | - Fyodor D. Urnov, Edward J. Rebar, Michael C. Holmes, H. Steve Zhang & Philip D. Gregory, "Genome editing with engineered zinc finger nucleases", Nature Reviews: Genetics, Nature Publishing Group, United Kingdom, United Kingdom , (20100901), vol. 11, no. 9, doi:10.1038/nrg2842, ISSN 1471-0064, pages 636 - 646, XP008150557 |
| OPPOSITION | - J. Keith Joung, Sander Jeffry D., "TALENs: a widely applicable technology for targeted genome editing", Nature Reviews Molecular Cell Biology, Nature Publishing Group UK, London, London, vol. 14, no. 1, doi:10.1038/nrm3486, ISSN 1471-0072, pages 49 - 55, XP055282847 |
| OPPOSITION | - Makarova Kira S; Haft Daniel H; Barrangou Rodolphe; Brouns Stan J J; Charpentier Emmanuelle; Horvath Philippe; Moineau Sylvain; Mojica Francisco J M; Wolf Yuri I; Yakunin Alexander F; van der Oost John; Koonin Eugene V, "Evolution and classification of the CRISPR-Cas systems", Nature Reviews Microbiology, NATURE PUBLISHING GROUP, GB, GB , (20110509), vol. 9, no. 6, doi:10.1038/nrmicro2577, ISSN 1740-1526, pages 467 - 477, XP009155547 |
| OPPOSITION | - Lee Y-H; Judge A D; Seo D; Kitade M; Gómez-Quiroz L E; Ishikawa T; Andersen J B; Kim B-K; Marquardt J U; Raggi C; Avital I; Conner E A; MacLachlan I; Factor V M; Thorgeirsson S S, "Molecular targeting of CSN5 in human hepatocellular carcinoma: a mechanism of therapeutic response", Oncogene, Nature Publishing Group UK, London, London , (20110418), vol. 30, no. 40, doi:10.1038/onc.2011.126, ISSN 0950-9232, pages 4175 - 4184, XP037744023 |
| OPPOSITION | - Wei Tuo, Cheng Qiang, Min Yi-Li, Olson Eric N., Siegwart Daniel J., "Systemic nanoparticle delivery of CRISPR-Cas9 ribonucleoproteins for effective tissue specific genome editing", Nature Communications, (20201201), vol. 11, no. 1, doi:10.1038/s41467-020-17029-3, XP055873647 |
| OPPOSITION | - Nomura S; Merched A; Nour E; Dieker C; Oka K; Chan L, "Low-density lipoprotein receptor gene therapy using helper-dependent adenovirus produces long-term protection against atherosclerosis in a mouse model of familial hypercholesterolemia", Gene Therapy, Nature Publishing Group, London, GB, GB , (20040722), vol. 11, no. 20, doi:10.1038/sj.gt.3302310, ISSN 0969-7128, pages 1540 - 1548, XP037770779 |
| OPPOSITION | - Rossi J J, "RNAi therapeutics: SNALPing siRNAs in vivo", Gene Therapy, Nature Publishing Group, London, GB, GB , (20051006), vol. 13, no. 7, doi:10.1038/sj.gt.3302661, ISSN 0969-7128, pages 583 - 584, XP037773059 |
| OPPOSITION | - Le Bec C; Douar A M, "Gene Therapy Progress and Prospects - Vectorology: design and production of expression cassettes in AAV vectors", Gene Therapy, Nature Publishing Group, London, GB, GB , (20060202), vol. 13, no. 10, doi:10.1038/sj.gt.3302724, ISSN 0969-7128, pages 805 - 813, XP037770951 |
| OPPOSITION | - Chen C-C; Ko T-M; Ma H-I; Wu H-L; Xiao X; Li J; Chang C-M; Wu P-Y; Chen C-H; Han J-M; Yu C-P; Jeng K-S; Hu C-P; Tao M-H, "Long-term inhibition of hepatitis B virus in transgenic mice by double-stranded adeno-associated virus 8-delivered short hairpin RNA", Gene Therapy, Nature Publishing Group, London, GB, GB , (20060824), vol. 14, no. 1, doi:10.1038/sj.gt.3302846, ISSN 0969-7128, pages 11 - 19, XP037772840 |
| OPPOSITION | - Thomas Laumonier, Sheng Yang, Stephane Konig, Christine Chauveau, Ignacio Anegon, Pierre Hoffmeyer and Jacques Menetrey, "Lentivirus mediated HO-1 gene transfer enhances myogenic precursor cell survival after autologous transplantation in pig", Molecular Therapy, Elsevier Inc., US, US , (20080201), vol. 16, no. 2, doi:10.1038/sj.mt.6300354, ISSN 1525-0016, pages 404 - 410, XP008159717 |
| OPPOSITION | - Hirotaka Ebina, Naoko Misawa, Yuka Kanemura, Yoshio Koyanagi, "Harnessing the CRISPR/Cas9 system to disrupt latent HIV-1 provirus", Scientific Reports, vol. 3, doi:10.1038/srep02510, pages 1 - 7, XP055110157 |
| OPPOSITION | - NATHWANI et al., "Adenovirus-Associated Virus Vector- Mediated Gene Transfer in Hemophilia B", N Engl J Med., (20111222), vol. 365, no. 25, doi:10.1056/NEJMoa1108046, pages 2357 - 2365, XP055079598 |
| OPPOSITION | - Anonymous, "CLINICAL STUDY PROTOCOL - CONFIDENTIAL: ALN-TTR01-001; A Phase 1, Randomized, Single-Blind, Placebo-Controlled, Dose Escalation Trial to Evaluate the Safety and Tolerability of a Single Dose of Intravenous ALN-TTR01 in Patients with TTR Amyloidosis", Alnylam Pharmaceuticals, doi:10.1056/NEJMoal208760, (20091216), Alnylam Pharmaceuticals, (20240312), XP093140407 |
| OPPOSITION | - MOEHLE E A, ET AL, "Targeted gene addition into a specified location in the human genome using designed zinc finger nucleases + corrections", Proceedings of the National Academy of Sciences, National Academy of Sciences, (20070201), vol. 104, no. 9, doi:10.1073/PNAS.0611478104, ISSN 0027-8424, pages 3055 - 3060, 6090, XP002518477 |
| OPPOSITION | - BEUMER KELLY J ET AL, "Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases.", PNAS, Proceedings of the National Academy of Sciences, National Academy of Sciences, US, US , (20081216), vol. 105, no. 50, doi:10.1073/pnas.0810475105, ISSN 1091-6490, pages 19821 - 19826, XP002782686 |
| OPPOSITION | - K. T. Love, K. P. Mahon, C. G. Levins, K. A. Whitehead, W. Querbes, J. R. Dorkin, J. Qin, W. Cantley, L. L. Qin, T. Racie, M. Frank-Kamenetsky, K. N. Yip, R. Alvarez, D. W. Y. Sah, A. De Fougerolles, K. Fitzgerald, V. Koteliansky, A. Akinc, R. Langer, D. G. Anderson, "Lipid-like materials for low-dose, in vivo gene silencing", Proceedings of the National Academy of Sciences, National Academy of Sciences, (20100202), vol. 107, no. 5, doi:10.1073/pnas.0910603106, ISSN 00278424, pages 1864 - 1869, XP055077922 |
| OPPOSITION | - Bruno Tinland, Et Al, "The T-DNA-linked VirD2 protein contains two distinct functional nuclear localization signals", Proc. Natl. Acad. Sci., (19920801), vol. 89, doi:10.1073/pnas.89.16.7442, pages 7442 - 7446, XP055254906 |
| OPPOSITION | - Feng Liang, Han Mingguang, Romanienko Peter J, Jasin Maria, Radding Charles M, "Homology-directed repair is a major double-strand break repair pathway in mammalian cells", Genetics and approved February, (19980401), vol. 95, no. 18, doi:10.1073/pnas.95.9.5172, pages 5172 - 5177, XP055502633 |
| OPPOSITION | - Y.-S. Dai, Et Al, "The Transcription Factors GATA4 and dHAND Physically Interact to Synergistically Activate Cardiac Gene Expression through a p300-dependent Mechanism", Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, US, US , (20020705), vol. 277, no. 27, doi:10.1074/jbc.M202490200, ISSN 0021-9258, pages 24390 - 24398, XP055251716 |
| OPPOSITION | - I.-H. Park, Et Al, "Regulation of Ribosomal S6 Kinase 2 by Mammalian Target of Rapamycin", Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, US, US , (20020823), vol. 277, no. 35, doi:10.1074/jbc.M204080200, ISSN 0021-9258, pages 31423 - 31429, XP055251717 |
| OPPOSITION | - S. L. Planey, Et Al, "Inhibition of Glucocorticoid-induced Apoptosis in 697 Pre-B Lymphocytes by the Mineralocorticoid Receptor N-terminal Domain", Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, US, US , (20021101), vol. 277, no. 44, doi:10.1074/jbc.M205085200, ISSN 0021-9258, pages 42188 - 42196, XP055251719 |
| OPPOSITION | - Alec E. Hodel, Michelle T. Harreman, Kanika F. Pulliam, Mary Elizabeth Harben, Jordan S. Holmes, Mary R. Hodel, Keith M. Berland, Anita H. Corbett, "Nuclear Localization Signal Receptor Affinity Correlates with in Vivo Localization in Saccharomyces cerevisiae", Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, US, US , (20060818), vol. 281, no. 33, doi:10.1074/jbc.M601718200, ISSN 0021-9258, pages 23545 - 23556, XP055502581 |
| OPPOSITION | - C. Dingwall, Jacqueline Robbins, Stephen M Dilworth, Bruce Roberts, William D Richardson¢, "The nucleoplasmin nuclear location sequence is larger and more complex than that of SV-40 large T antigen", The journal of cell biology, The Rockefeller University Press, US, US , (19880901), vol. 107, no. 3, doi:10.1083/jcb.107.3.841, ISSN 0021-9525, pages 841 - 849, XP055514316 |
| OPPOSITION | - Marjorie Robbins, Adam Judge, Ellen Ambegia, Catherine Choi, Ed Yaworski, Lorne Palmer, Kevin Mcclintock, Ian Maclachlan, "Misinterpreting the Therapeutic Effects of Small Interfering RNA Caused by Immune Stimulation", Human Gene Therapy, Mary Ann Liebert, (20081001), vol. 19, no. 10, doi:10.1089/hum.2008.131, ISSN 10430342, pages 991 - 999, XP055046701 |
| OPPOSITION | - Händel E M, Gellhaus K, Khan K, Bednarski C, Cornu T I, Müller-Lerch F, Kotin R M, Heilbronn R, Cathomen T., "Versatile and efficient genome editing in human cells by combining zinc-finger nucleases with adeno-associated viral vectors.", HUMAN GENE THERAPY, Mary Ann Liebert, Inc. Publishers, GB, GB , (20120301), vol. 23, no. 3, doi:10.1089/hum.2011.140, ISSN 1043-0342, pages 321 - 329, XP002730956 |
| OPPOSITION | - KASSIM et al., "Adeno-Associated Virus Serotype 8 Gene Therapy Leads to Significant Lowering of Plasma Cholesterol Levels in Humanized Mouse Models of Homozygous and Heterozygous Familial Hypercholesterolemia", Human Gene Therapy, (20130100), vol. 24, doi:10.1089/hum.2012.108, pages 19 - 26, XP055348442 |
| OPPOSITION | - SALOMON S, PUCHTA H, "Capture of genomic and T-DNA sequences during double-strand break repair in somatic plant cells", The EMBO journal / European Molecular Biology Organization, IRL Press, Oxford, Oxford , (19981015), vol. 17, no. 20, doi:10.1093/emboj/17.20.6086, ISSN 0261-4189, pages 6086 - 6095, XP002259898 |
| OPPOSITION | - Holger Puchta, "The repair of double-strand breaks in plants: mechanisms and consequences for genome evolution", JOURNAL OF EXPERIMENTAL BOTANY, OXFORD UNIVERSITY PRESS, GB, GB , (20041122), vol. 56, no. 409, doi:10.1093/jxb/eri025, ISSN 0022-0957, pages 1 - 14, XP002394840 |
| OPPOSITION | - S. J. Orlando, Santiago Y., Dekelver R. C., Freyvert Y., Boydston E. A., Moehle E. A., Choi V. M., Gopalan S. M., Lou J. F., Li J., Miller J. C., Holmes M. C., Gregory P. D., Urnov F. D., Cost G. J., "Zinc-finger nuclease-driven targeted integration into mammalian genomes using donors with limited chromosomal homology", Nucleic Acids Research, Oxford University Press, GB, GB , (20100801), vol. 38, no. 15, doi:10.1093/nar/gkq512, ISSN 0305-1048, pages e152 - e152 - 15, XP055076783 |
| OPPOSITION | - Tomas Cermak, Erin L Doyle, Michelle Christian, Li Wang, Yong Zhang, Clarice Schmidt, Joshua A Baller, Nikunj V Somia, Adam J Bogdanove and Daniel F Voytas, "Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting.", Nucleic Acids research, Oxford University Press, GB, GB , (20110701), vol. 39, no. 12, doi:10.1093/nar/gkr218, ISSN 1362-4962, pages 1 - 11, XP002659530 |
| OPPOSITION | - J. E. Dicarlo, J. E. Norville, P. Mali, X. Rios, J. Aach, G. M. Church, "Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems", Nucleic Acids Research, Information Retrieval Ltd., (20130401), vol. 41, no. 7, doi:10.1093/nar/gkt135, ISSN 03051048, pages 4336 - 4343, XP055086617 |
| OPPOSITION | - M. Maresca, Lin V. G., Guo N., Yang Y., "Obligate Ligation-Gated Recombination (ObLiGaRe): Custom-designed nuclease-mediated targeted integration through nonhomologous end joining", Genome Research, Cold Spring Harbor Laboratory Press, (20130301), vol. 23, no. 3, doi:10.1101/gr.145441.112, ISSN 10889051, pages 539 - 546, XP055077484 |
| OPPOSITION | - M. Shieh, Et Al, "Nuclear targeting of the maize R protein requires two nuclear localization sequences", PLANT PHYSIOLOGY, (19930201), vol. 101, no. 2, doi:10.1104/pp.101.2.353, pages 353 - 361, XP055241771 |
| OPPOSITION | - Ming Luo, C. W. Mark Pang, Alison E. Gerken, Thomas G. Brock, "Multiple Nuclear Localization Sequences Allow Modulation of 5-Lipoxygenase Nuclear Import", TRAFFIC, MUNKSGAARD,, DK, DK , (20040901), vol. 5, no. 11, doi:10.1111/j.1600-0854.2004.00227.x, ISSN 1398-9219, pages 847 - 854, XP055267733 |
| OPPOSITION | - PORTEUS M H, BALTIMORE D, "Chimeric nucleases stimulate gene targeting in human cells", Science, American Association for the Advancement of Science, US, US , (20030502), vol. 300, doi:10.1126/science.1078395, ISSN 0036-8075, page 763, XP002974231 |
| OPPOSITION | - BIBIKOVA M, ET AL., "Enhancing gene targeting with designed zinc finger nucleases", Science, American Association for the Advancement of Science, US, US , (20030502), vol. 300, doi:10.1126/science.1079512, ISSN 0036-8075, page 764, XP002974267 |
| OPPOSITION | - GEURTS ARON M ET AL, "Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases", Science, American Association for the Advancement of Science, US, US , (20090724), vol. 325, no. 5939, doi:10.1126/SCIENCE.1172447, ISSN 0036-8075, page 433, XP002580718 |
| OPPOSITION | - JINEK et al., "A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity", Science, (20120817), vol. 337, doi:10.1126/science.1225829, pages 816 - 821, XP055229606 |
| OPPOSITION | - M. Jinek, K. Chylinski, I. Fonfara, M. Hauer, J. A. Doudna, E. Charpentier, "A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity", Science, American Association for the Advancement of Science, US, US , (20120817), vol. 337, no. 6096, doi:10.1126/science.1225829, ISSN 0036-8075, pages 816 - 821, XP055229606 |
| OPPOSITION | - BROUNS, "A Swiss Army Knife of Immunity", Science, (20120817), vol. 337, doi:10.1126/science.1227253, pages 808 - 809, XP055113718 |
| OPPOSITION | - S. J. J. Brouns, "A Swiss Army Knife of Immunity", Science, American Association for the Advancement of Science, (20120817), vol. 337, no. 6096, doi:10.1126/science.1227253, ISSN 00368075, pages 808 - 809, XP055113718 |
| OPPOSITION | - CONG et al., "Multiplex Genome Engineering Using CRISPR/Cas Systems", Science, (20130103), vol. 339, doi:10.1126/science.1231143, pages 819 - 823, XP055300065 |
| OPPOSITION | - P. Mali, L. Yang, K. M. Esvelt, J. Aach, M. Guell, J. E. Dicarlo, J. E. Norville, G. M. Church, "RNA-Guided Human Genome Engineering via Cas9", Science, American Association for the Advancement of Science, (20130215), vol. 339, no. 6121, doi:10.1126/science.1232033, ISSN 00368075, pages 823 - 826, XP055111247 |
| OPPOSITION | - Kinnevey, Et Al, "Emergence of Sequence Type 779 Methicillin-Resistant Staphylococcus aureus Harboring a Novel Pseudo Staphylococcal Cassette Chromosome mec (SCCmec)-SCC-SCCcRISPR Composite Element 1n Irish Hospitals", Antimicrob Agents Chemother, (20130101), vol. 57, no. 1, doi:10.1128/AAC.01689-12, pages 524 - 531, XP055338796 |
| OPPOSITION | - J. S. Lee, A. G. Hadjipanayis, S. L. Welkos, "Venezuelan Equine Encephalitis Virus-Vectored Vaccines Protect Mice against Anthrax Spore Challenge", Infection and immunity, American Society for Microbiology, US, US , (20030301), vol. 71, no. 3, doi:10.1128/IAI.71.3.1491-1496.2003, ISSN 0019-9567, pages 1491 - 1496, XP055519514 |
| OPPOSITION | - ROUET P., SMIH F., JASIN M., "Introduction of double-strand breaks into the genome of mouse cells by expression of a rare-cutting endonuclease.", MOLECULAR AND CELLULAR BIOLOGY, American Society for Pharmacology and Experimental Therapeutics, US, US , (19941201), vol. 14., no. 12., doi:10.1128/mcb.14.12.8096, ISSN 0270-7306, pages 8096 - 8106, XP000572018 |
| OPPOSITION | - D. G. Miller, L. M. Petek, D. W. Russell, "Human Gene Targeting by Adeno-Associated Virus Vectors Is Enhanced by DNA Double-Strand Breaks", MOLECULAR AND CELLULAR BIOLOGY, American Society for Pharmacology and Experimental Therapeutics, US, US , (20030515), vol. 23, no. 10, doi:10.1128/MCB.23.10.3550-3557.2003, ISSN 0270-7306, pages 3550 - 3557, XP055502623 |
| OPPOSITION | - FISCHER-FANTUZZI L; VESCO C, "Cell-dependent efficiency of reiterated nuclear signals in a mutant simian virus 40 oncoprotein targeted to the nucleus", MOLECULAR AND CELLULAR BIOLOGY, American Society for Pharmacology and Experimental Therapeutics, US, US , (19981201), vol. 8, no. 12, doi:10.1128/MCB.8.12.549, ISSN 0270-7306, pages 5495 - 5503, XP009140368 |
| OPPOSITION | - N Rudin, J E Haber, "Efficient repair of HO-induced chromosomal breaks in Saccharomyces cerevisiae by recombination between flanking homologous sequences", Molecular and Cellular Biology, American Society for Microbiology, United States, United States , doi:10.1128/MCB.8.9.3918, (19880901), pages 3918 - 3928, Molecular and Cellular Biology, URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC365451/pdf/molcellb00069-0358.pdf, XP055502440 |
| OPPOSITION | - Michael R. Lieber, "The Mechanism of Double-Strand DNA Break Repair by the Nonhomologous DNA End-Joining Pathway", ANNUAL REVIEW OF BIOCHEMISTRY, PALTO ALTO, CA, US, US , (20100607), vol. 79, no. 1, doi:10.1146/annurev.biochem.052308.093131, ISSN 0066-4154, pages 181 - 211, XP055340167 |
| OPPOSITION | - Ketola Anna, Hinkkanen Ari, Yongabi Felicitas, Furu Petra, Määttä Ann-Marie, Liimatainen Timo, Pirinen Risto, Björn Marko, Hakkarainen Tanja, Mäkinen Kimmo, Wahlfors Jarmo, Pellinen Riikka, "Oncolytic Semliki Forest Virus Vector as a Novel Candidate against Unresectable Osteosarcoma", Cancer research, American Association for Cancer Research, 2019 San Antonio Breast Cancer Symposium, San Antonio, Texas, 2019 San Antonio Breast Cancer Symposium, San Antonio, Texas , (20081015), vol. 68, no. 20, doi:10.1158/0008-5472.CAN-08-0251, ISSN 0008-5472, pages 8342 - 8350, XP055983305 |
| OPPOSITION | - JUDGE ADAM D ET AL, "Confirming the RNAi-mediated mechanism of action of siRNA-based cancer therapeutics in mice", The Journal of Clinical Investigation, B M J Group, GB, GB , (20090301), vol. 119, no. 3, doi:10.1172/JCI37515, ISSN 0021-9738, pages 661 - 673, XP002618489 |
| OPPOSITION | - Anguela Xavier M.; Sharma Rajiv; Doyon Yannick; Miller Jeffrey C.; Li Hojun; Haurigot Virginia; Rohde Michelle E.; Wong Sunnie Y.; Davidson Robert J.; Zhou Shangzhen; Gregory Philip D.; Holmes Michael C.; High Katherine A., "Robust ZFN-mediated genome editing in adult hemophilic mice", Blood, American Society of Hematology, US, US , (20131107), vol. 122, no. 19, doi:10.1182/blood-2013-04-497354, ISSN 0006-4971, pages 3283 - 3287, XP086691844 |
| OPPOSITION | - Jensen Nanna M; Dalsgaard Trine; Jakobsen Maria; Nielsen Roni R; SÃ ̧rensen Charlotte B; Bolund Lars; Jensen Thomas G, "An update on targeted gene repair in mammalian cells: methods and mechanisms", Journal of Biomedical Science, Kluwer Academic Publishers, Do, Do , (20110202), vol. 18, no. 1, doi:10.1186/1423-0127-18-10, ISSN 1423-0127, pages 1 - 10, XP021085735 |
| OPPOSITION | - Brice V McConnell;Karen Koto;Arthur Gutierrez-Hartmann, "Nuclear and cytoplasmic LIMK1 enhances human breast cancer progression", MOLECULAR CANCER, BIOMED CENTRAL, LONDON, GB, GB , (20110618), vol. 10, no. 1, doi:10.1186/1476-4598-10-75, ISSN 1476-4598, pages 1 - 13, XP021103688 |
| OPPOSITION | - Wuyang Zhu;Jiangjiao Li;Li Tang;Huanqin Wang;Jia Li;Juanjuan Fu;Guodong Liang, "Glycoprotein is enough for sindbis virus-derived DNA vector to express heterogenous genes", VIROLOGY JOURNAL, BIOMED CENTRAL, LONDON, GB, GB , (20110710), vol. 8, no. 1, doi:10.1186/1743-422X-8-344, ISSN 1743-422X, page 344, XP021105747 |
| OPPOSITION | - Kira S Makarova;L Aravind;Yuri I Wolf;Eugene V Koonin, "Unification of Cas protein families and a simple scenario for the origin and evolution of CRISPR-Cas systems", Biology Direct, BioMed Central, (20110714), vol. 6, no. 1, doi:10.1186/1745-6150-6-38, ISSN 1745-6150, pages 1 - 27, XP021105829 |
| OPPOSITION | - Ari E. Friedland, Reshica Baral, Pankhuri Singhal, Katherine Loveluck, Shen Shen, Minerva Sanchez, Eugenio Marco, Gregory M. Gotta, Morgan L. Maeder, Edward M. Kennedy, Anand V. R. Kornepati, Alexander Sousa, Mckensie A. Collins, Hari Jayaram, Bryan R. Cullen, David Bumcrot, "Characterization of Staphylococcus aureus Cas9: a smaller Cas9 for all-in-one adeno-associated virus delivery and paired nickase applications", Genome Biology, (20151201), vol. 16, no. 1, doi:10.1186/s13059-015-0817-8, XP055347837 |
| OPPOSITION | - Chieh Chang, Yi-Wen Hsieh, Bluma J. Lesch, Cornelia I. Bargmann, Chiou-Fen Chuang, "Microtubule-based localization of a synaptic calcium-signaling complex is required for left-right neuronal asymmetry in C. elegans", Development, The Company of Biologists Ltd., GB, GB , (20110815), vol. 138, no. 16, doi:10.1242/dev.069740, ISSN 0950-1991, pages 3509 - 3518, XP055502632 |
| OPPOSITION | - Hruscha Alexander; Krawitz Peter; Rechenberg Alexandra; Heinrich Verena; Hecht Jochen; Haass Christian; Schmid Bettina, "Efficient CRISPR/Cas9 genome editing with low off-target effects in zebrafish", Development, The Company of Biologists Ltd., GB, GB , (20131201), vol. 140, no. 24, doi:10.1242/dev.099085, ISSN 0950-1991, pages 4982 - 4987, XP009177513 |
| OPPOSITION | - Keisuke Sato, Et Al, "Generation of Adeno-Associated Virus Vector Enabling Functional Expression of Oxytocin Receptor and Fluorescence Marker Genes Using the Human eIF4G Internal Ribosome Entry Site Element", Bioscience, Biotechnology, and Biochemistry, Japan Society for Bioscience, Biotechnology, and Agrochemistry, JP, JP , (20090923), vol. 73, no. 9, doi:10.1271/bbb.90287, ISSN 0916-8451, pages 2145 - 2148, XP055487142 |
| OPPOSITION | - Christopher S. Raymond, Philippe Soriano, "High-Efficiency FLP and ΦC31 Site-Specific Recombination in Mammalian Cells", PLOS ONE, Public Library of Science, US, US , (20070101), vol. 2, no. 1, doi:10.1371/journal.pone.0000162, ISSN 1932-6203, pages e162/1 - 4, XP055069825 |
| OPPOSITION | - Eric Campeau, Et Al, "A Versatile Viral System for Expression and Depletion of Proteins in Mammalian Cells. article e6529", PLoS ONE, (20090801), vol. 4, no. 8, doi:10.1371/journal.pone.0006529, pages 1 - 18, XP055251120 |
| OPPOSITION | - Gabriella Cotugno, Patrizia Annunziata, Maria Vittoria Barone, Marianthi Karali, Sandro Banfi, Alberto Auricchio, "Impact of Age at Administration, Lysosomal Storage, and Transgene Regulatory Elements on AAV2/8-Mediated Rat Liver Transduction", PLoS ONE, vol. 7, no. 3, doi:10.1371/journal.pone.0033286, page e33286, XP055279601 |
| OPPOSITION | - Woong Y. Hwang, Yanfang Fu, Deepak Reyon, Morgan L. Maeder, Prakriti Kaini, Jeffry D. Sander, J. Keith Joung, Randall T. Peterson, Jing-Ruey Joanna Yeh, "Heritable and Precise Zebrafish Genome Editing Using a CRISPR-Cas System", PLoS ONE, vol. 8, no. 7, doi:10.1371/journal.pone.0068708, page e68708, XP055196397 |
| OPPOSITION | - WEBER et al., "AAV-Mediated Delivery of Zinc Finger Nucleases Targeting Hepatitis B Virus Inhibits Active Replication", Plos One, (20140514), vol. 9, no. 5, doi:10.1371/journal.pone.0097579, pages 1 - 14, XP055155850 |
| OPPOSITION | - James A. Gagnon, Eivind Valen, Summer B. Thyme, Peng Huang, Laila Ahkmetova, Andrea Pauli, Tessa G. Montague, Steven Zimmerman, Constance Richter, Alexander F. Schier, "Efficient Mutagenesis by Cas9 Protein-Mediated Oligonucleotide Insertion and Large-Scale Assessment of Single-Guide RNAs", PLoS ONE, vol. 9, no. 5, doi:10.1371/journal.pone.0098186, page e98186, XP055389880 |
| OPPOSITION | - HOOPER et al., "Anti-PCSK9 therapies for the treatment of hypercholesterolemia", Expert Opinion on Biological Therapy, (20121217), vol. 13, no. 3, doi:10.1517/14712598.2012.748743, pages 429 - 435, XP009172526 |
| OPPOSITION | - S. K. Upadhyay, J. Kumar, A. Alok, R. Tuli, "RNA-Guided Genome Editing for Target Gene Mutations in Wheat", G3: Genes|Genomes|Genetics, Genetics Society of America, (20131209), vol. 3, no. 12, doi:10.1534/g3.113.008847, ISSN 21601836, pages 2233 - 2238, XP055195466 |
| OPPOSITION | - Michelle Christian et al, "Targeting DNA Double-Strand Breaks with TAL Effector Nucleases (plus supporting information)", GENETICS, GENETICS SOCIETY OF AMERICA, AUSTIN, TX, US, US , (20101001), vol. 186, no. 2, doi:10.1534/GENETICS.110.120717, ISSN 0016-6731, pages 757 - 761, 1SI-8SI, XP002632806 |
| OPPOSITION | - D. Carroll, "Genome Engineering With Zinc-Finger Nucleases", Genetics, Genetics Society of America [etc.], (20110801), vol. 188, no. 4, doi:10.1534/genetics.111.131433, ISSN 00166731, pages 773 - 782, XP055171682 |
| OPPOSITION | - S. J. Gratz, A. M. Cummings, J. N. Nguyen, D. C. Hamm, L. K. Donohue, M. M. Harrison, J. Wildonger, K. M. O'connor-Giles, "Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease", Genetics, Genetics Society of America [etc.], (20130801), vol. 194, no. 4, doi:10.1534/genetics.113.152710, ISSN 00166731, pages 1029 - 1035, XP055102690 |
| OPPOSITION | - Z. Yu, M. Ren, Z. Wang, B. Zhang, Y. S. Rong, R. Jiao, G. Gao, "Highly Efficient Genome Modifications Mediated by CRISPR/Cas9 in Drosophila (includes Supporting Information)", GENETICS, GENETICS SOCIETY OF AMERICA, AUSTIN, TX, US, US , (20130901), vol. 195, no. 1, doi:10.1534/genetics.113.153825, ISSN 0016-6731, pages 289 - 291,1-14, XP055242275 |
| OPPOSITION | - Nicholas Lyssenko, Hanna-Rose Wendy, Schlegel Robert, "Cognate putative nuclear localization signal effects strong nuclear localization of a GFP reporter and facilitates gene expression studies in Caenorhabditis elegans", Biotechniques, Informa Healthcare, US, US , (20071101), vol. 43, no. 5, doi:10.2144/000112615, ISSN 0736-6205, pages 596 - 600, XP055268137 |
| OPPOSITION | - BANASZEWSKA ANNA, PIECHOTA MICHAL, PLEWA ROBERT, "Proprotein convertase subtilisin/kexin type 9: A new target molecule for gene therapy", CELLULAR & MOLECULAR BIOLOGY LETTERS, UNIVERSITY OF WROCAW. INSTITUTE OF BIOCHEMISTRY, WROCAW; PL, PL, PL , (20120601), vol. 17, no. 2, doi:10.2478/S11658-012-0006-7, ISSN 1425-8153, pages 228 - 239, XP002732020 |
| OPPOSITION | - Gómez-Aguado Itziar, Rodríguez-Castejón Julen, Vicente-Pascual Mónica, Rodríguez-Gascón Alicia, Solinís María Ángeles, Del Pozo-Rodríguez Ana, "Nanomedicines to Deliver mRNA: State of the Art and Future Perspectives", Nanomaterials, vol. 10, no. 2, doi:10.3390/nano10020364, page 364, XP055911347 |
| OPPOSITION | - Martin Jinek, Alexandra East, Aaron Cheng, Steven Lin, Enbo Ma, Jennifer Doudna, "RNA-programmed genome editing in human cells.", eLife, eLife Sciences Publications Ltd., GB, GB , (20130129), vol. 2, doi:10.7554/eLife.00471, ISSN 2050-084X, pages e00471 - e00471-9, XP002699851 |
| OPPOSITION | - Allison Lange, Ryan E. Mills, Christopher J. Lange, Murray Stewart, Scott E. Devine, Anita H. Corbett, "Classical nuclear localization signals: Definition, function, and interaction with importin [alpha]", Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, US, US , (20070223), vol. 282, no. 8, doi:10.1074/JBC.R600026200, ISSN 0021-9258, pages 5101 - 5105, XP002674492 |
