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Controllable Transcription - EP3545079

The patent EP3545079 was granted to Cambridge Enterprise on Jun 11, 2025. The application was originally filed on Nov 24, 2017 under application number EP17807909A. The patent is currently recorded with a legal status of "Patent Maintained As Amended".

EP3545079

CAMBRIDGE ENTERPRISE
Application Number
EP17807909A
Filing Date
Nov 24, 2017
Status
Patent Maintained As Amended
May 9, 2025
Grant Date
Jun 11, 2025
External Links
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OETKEJan 5, 2023WALLINGER RICKER SCHLOTTER TOSTMANNADMISSIBLE

Patent Citations (6) New

Patent citations refer to prior patents cited during different phases such as opposition or international search.

Citation PhasePublication Number
EXAMINATIONWO2010109187
INTERNATIONAL-SEARCH-REPORTWO2010117464
INTERNATIONAL-SEARCH-REPORTWO2016183041
OPPOSITIONCN112852877
OPPOSITIONWO2018096343
OTHERWO2010109187

Non-Patent Literature (NPL) Citations (47) New

NPL citations refer to non-patent references such as research papers, articles, or other publications cited during examination or opposition phases.

Citation PhaseReference Text
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EXAMINATION- Ying Pei ET AL, "A platform for rapid generation of single and multiplexed reporters in human iPSC lines", Scientific Reports, (20150317), vol. 5, no. 1, doi:10.1038/srep09205, pages 1 - 10, XP055713337
INTERNATIONAL-SEARCH-REPORT- Andrew Petersen ET AL, "UW-Madison Human Stem Cell Gene Editing Service A campus resource for applying CRISPR/Cas9in human pluripotent stem cells", (20160901), URL: https://www2.waisman.wisc.edu/Core-CMN/GeneEditingServiceSept2016.pdf, (20180117), XP055441585 [A] 1-30 * the whole document *
INTERNATIONAL-SEARCH-REPORT- KUN QIAN ET AL, "A Simple and Efficient System for Regulating Gene Expression in Human Pluripotent Stem Cells and Derivatives : Inducible Transgene Expression in hPSCs and Derivatives", STEM CELLS., US, (20140417), vol. 32, no. 5, doi:10.1002/stem.1653, ISSN 1066-5099, pages 1230 - 1238, XP055441569 [A] 1-30 * the whole document *
INTERNATIONAL-SEARCH-REPORT- MATTHIAS PAWLOWSKI ET AL, "Inducible and Deterministic Forward Programming of Human Pluripotent Stem Cells into Neurons, Skeletal Myocytes, and Oligodendrocytes", STEM CELL REPORTS, United States, (20170401), vol. 8, no. 4, doi:10.1016/j.stemcr.2017.02.016, ISSN 2213-6711, pages 803 - 812, XP055441144 [XP] 1-30 * the whole document *
INTERNATIONAL-SEARCH-REPORT- EIRINI P PAPAPETROU ET AL, "Gene Insertion Into Genomic Safe Harbors for Human Gene Therapy", MOLECULAR THERAPY, US, (20160401), vol. 24, no. 4, doi:10.1038/mt.2016.38, ISSN 1525-0016, pages 678 - 684, XP055441242 [A] 1-30 * the whole document *
INTERNATIONAL-SEARCH-REPORT- MICHEL SADELAIN ET AL, "Safe harbours for the integration of new DNA in the human genome", NATURE REVIEWS CANCER, (20110101), vol. 12, no. 1, doi:10.1038/nrc3179, ISSN 1474-175X, pages 51 - 58, XP055018235 [A] 1-30 * the whole document *
INTERNATIONAL-SEARCH-REPORT- R. C. DEKELVER ET AL, "Functional genomics, proteomics, and regulatory DNA analysis in isogenic settings using zinc finger nuclease-driven transgenesis into a safe harbor locus in the human genome", GENOME RESEARCH, US, (20100801), vol. 20, no. 8, doi:10.1101/gr.106773.110, ISSN 1088-9051, pages 1133 - 1142, XP055265374 [A] 1-30 * the whole document *
INTERNATIONAL-SEARCH-REPORT- ALESSANDRO BERTERO ET AL, "Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs", DEVELOPMENT, GB, (20161129), vol. 143, no. 23, doi:10.1242/dev.138081, ISSN 0950-1991, pages 4405 - 4418, XP055421687 [XP] 1-30 * the whole document *
OPPOSITION- Anonymous, "Tet-Off® and Tet-On® Gene Expression Systems User Manual", Clontech, (20121001), Clontech , (20230123), XP093016642
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OPPOSITION- LI, S. et al., "Overview of the reporter genes and reporter mouse models", Animal Model Exp Med, (20180000), vol. 1, no. 1, doi:10.1002/ame2.12008, pages 29 - 35, XP093000732
OPPOSITION- BEARD, C. et al., "Efficient method to generate single-copy transgenic mice by site-specific integration in embryonic stem cells", Genesis, (20060000), vol. 44, no. 1, doi:10.1002/gene.20180, pages 23 - 28, XP002464119
OPPOSITION- Pfaff Nils, Lachmann Nico, Ackermann Mania, Kohlscheen Saskia, Brendel Christian, Maetzig Tobias, Niemann Heiner, Antoniou Michael N., Grez Manuel, Schambach Axel, Cantz Tobias, Moritz Thomas, "A ubiquitous chromatin opening element prevents transgene silencing in pluripotent stem cells and their differentiated progeny", Stem Cells, Wiley, (20130301), vol. 31, no. 3, doi:10.1002/stem.1316, ISSN 1066-5099, pages 488 - 499, XP055775314
OPPOSITION- Sim Xiuli, Cardenas-Diaz Fabian L., French Deborah L., Gadue Paul, "A Doxycycline-Inducible System for Genetic Correction of iPSC Disease Models", Sim Xiuli, Cardenas-Diaz Fabian L., French Deborah L., Gadue Paul, Holger Erfle, Protein Chromatography : Methods and Protocols, New York, NY, Springer New York, (20140101), vol. 1353, pages 13 - 23, doi:10.1007/7651_2014_179, ISSN 1064-3745, ISBN 978-1-4939-6412-3, XP093016638
OPPOSITION- Jason A. Mills et al., "Chapter 12: Hematopoietic Differentiation of Pluripotent Stem Cells in Culture", Hematopoietic Stem Cell Protocols, (20140101), pages 181 - 194, doi:10.1007/978-1-4939-1133-2, ISBN 978-1-4939-1132-5, XP009556611
OPPOSITION- LI, P. et al., "Accelerated generation of oligodendrocyte progenitor cells from human induced pluripotent stem cells by forced expression of Sox10 and Olig2", Sci China Life Sci, (20160000), vol. 59, no. 11, doi:10.1007/s11427-016-0165-3, pages 1131 - 1138, XP036096059
OPPOSITION- HOCHEDLINGER, K. et al., "Ectopic expression of Oct-4 blocks progenitor- cell differentiation and causes dysplasia in epithelial tissues", Cell, (20050000), vol. 121, no. 3, doi:10.1016/j.cell.2005.02.018, pages 465 - 477, XP055083449
OPPOSITION- Jacob Hanna, Styliani Markoulaki, Patrick Schorderet, Bryce W. Carey, Caroline Beard, Marius Wernig, Menno P. Creyghton, Eveline J. Steine, John P. Cassady, Ruth Foreman, Christopher J. Lengner, Jessica A. Dausman, And Rudolf Jaenisch, "Direct reprogramming of terminally differentiated mature B lymphocytes to pluripotency", NIH Public Access Author Manuscript, (20080418), doi:10.1016/j.cell.2008.03.028, pages 1 - 23, XP055482309
OPPOSITION- PREMSRIRUT, P.K. et al., "A Rapid and Scalable System for Studying Gene Function in Mice Using Conditional RNA Interference", Cell, (20110000), vol. 145, no. 1, doi:10.1016/j.cell.2011.03.012, pages 145 - 158, XP055618671
OPPOSITION- AUBREY, B.J. et al., "An inducible lentiviral guide RNA platform enables the identification of tumor-essential genes and tumor-promoting mutations in vivo", Cell Rep, (20150000), vol. 10, no. 8, doi:10.1016/j.celrep.2015.02.002, pages 1422 - 1432, XP055303271
OPPOSITION- MUKHERJEE, S. et al., "Gene therapy for PIDs: progress, pitfalls and prospects", Gene, (20130000), vol. 525, no. 2, doi:10.1016/j.gene.2013.03.098, pages 174 - 181, XP028576970
OPPOSITION- Panagiotis Douvaras, Wang Jing, Zimmer Matthew, Hanchuk Stephanie, O’bara Melanie a., Sadiq Saud, Sim Fraser j., Goldman James, Fossati Valentina, "Efficient Generation of Myelinating Oligodendrocytes from Primary Progressive Multiple Sclerosis Patients by Induced Pluripotent Stem Cells", STEM CELL REPORTS, Cell Press, United States, United States , (20140812), vol. 3, no. 2, doi:10.1016/j.stemcr.2014.06.012, ISSN 2213-6711, pages 250 - 259, XP055411392
OPPOSITION- Honoré Stella M, Aybar Manuel J, Mayor Roberto, "Sox10 is required for the early development of the prospective neural crest in Xenopus embryos", Developmental Biology, ELSEVIER, AMSTERDAM, NL, AMSTERDAM, NL , (20030801), vol. 260, no. 1, doi:10.1016/S0012-1606(03)00247-1, ISSN 0012-1606, pages 79 - 96, XP093016553
OPPOSITION- PAPAPETROU, E.P. et al., "Gene Insertion Into Genomic Safe Harbors for Human Gene Therapy", Mol Ther, (20160000), vol. 24, no. 4, doi:10.1038/mt.2016.38, pages 678 - 684, XP055547341
OPPOSITION- CAREY, B. et al., "Single- gene transgenic mouse strains for reprogramming adult somatic cells", Nat Methods, (20100000), vol. 7, no. 1, doi:10.1038/nmeth.1410, pages 56 - 59, XP055074655
OPPOSITION- LOMBARDO, A. et al., "Site-specific integration and tailoring of cassette design for sustainable gene transfer", Nat Methods, (20110000), vol. 8, no. 10, doi:10.1038/nmeth.1674, pages 861 - 869, XP055602217
OPPOSITION- SADELAIN, M. et al., "Safe harbours for the integration of new DNA in the human genome", Nat Rev Cancer, (20120000), vol. 12, no. 1, doi:10.1038/nrc3179, pages 51 - 58, XP055018235
OPPOSITION- Gaspar-Maia Alexandre, Alajem Adi, Meshorer Eran, Ramalho-Santos Miguel, "Open chromatin in pluripotency and reprogramming", Nature Reviews Molecular Cell Biology, Nature Publishing Group UK, London, London, (20110101), vol. 12, no. 1, doi:10.1038/nrm3036, ISSN 1471-0072, pages 36 - 47, XP093198260
OPPOSITION- URLINGER, S. et al., "Exploring the sequence space for tetracycline- dependent transcriptional activators: novel mutations yield expanded range and sensitivity", Proc Natl Acad Sci U S A., (20000000), vol. 97, no. 14, doi:10.1073/pnas.130192197, pages 7963 - 7968, XP002320615
OPPOSITION- Klatt Denise, Cheng Erica, Hoffmann Dirk, Santilli Giorgia, Thrasher Adrian J., Brendel Christian, Schambach Axel, "Differential Transgene Silencing of Myeloid-Specific Promoters in the AAVS1 Safe Harbor Locus of Induced Pluripotent Stem Cell-Derived Myeloid Cells", HUMAN GENE THERAPY, Mary Ann Liebert, Inc. Publishers, GB, GB , (20200201), vol. 31, no. 3-4, doi:10.1089/hum.2019.194, ISSN 1043-0342, pages 199 - 210, XP093064824
OPPOSITION- NOWAK, C.M. et al., "Guide RNA engineering for versatile Cas9 functionality", Nucleic Acids Res, vol. 44, no. 20, doi:10.1093/nar/gkw908, (20161012), pages 9555 - 9564, XP055524584
OPPOSITION- Jennifer Easterbrook; Antonella Fidanza; Lesley M. Forrester, "Concise review: programming human pluripotent stem cells into blood", British Journal of Haematology, John Wiley, Hoboken, USA, Hoboken, USA, (20160321), vol. 173, no. 5, doi:10.1111/bjh.14010, ISSN 0007-1048, pages 671 - 679, XP071055884
OPPOSITION- GOSSEN, M. et al., "Transcriptional activation by tetracyclines in mammalian cells", Science, (19950000), vol. 268, no. 5218, doi:10.1126/science.7792603, pages 1766 - 1769, XP002176573
OPPOSITION- KENNEDY, K.A. et al., "Retinoic acid enhances skeletal muscle progenitor formation and bypasses inhibition by bone morphogenetic protein 4 but not dominant negative beta-catenin", BMC Biol, (20090000), vol. 7, doi:10.1186/1741-7007-7-67, page 67, XP021063657
OPPOSITION- BERTERO, A. et al., "Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs", Development, (20160000), vol. 143, no. 23, doi:10.1242/dev.138081, pages 4405 - 4418, XP055421687
OPPOSITION- CERBINI, T. et al., "Transcription activator-like effector nuclease (TALEN)-mediated CLYBL targeting enables enhanced transgene expression and one-step generation of dual reporter human induced pluripotent stem cell (iPSC) and neural stem cell (NSC) lines", PLoS One, (20150000), vol. 10, no. 1, doi:10.1371/journal.pone.0116032, page e0116032, XP055602218
OPPOSITION- Tan Lei, Hu, Li Yalan, Yang Lingchen, Cai Xiong, Liu Wei, He Jiayi, Wu Yingxin, Liu Tanbin, Wang Naidong, Yang, Adelstein Robert, Wang Aibing, Hu Wenhui, "Investigation of the molecular biology underlying the pronounced high gene targeting frequency at the Myh9 gene locus in mouse embryonic stem cells", PLOS ONE, Public Library of Science, US, US , (20200330), vol. 15, no. 3, doi:10.1371/journal.pone.0230126, ISSN 1932-6203, page e0230126, XP093131253
OPPOSITION- Merkert Sylvia, Martin Ulrich, "Site-Specific Genome Engineering in Human Pluripotent Stem Cells", International journal of molecular sciences, Molecular Diversity Preservation International (MDPI), Basel, CH, Basel, CH , (20160624), vol. 17, no. 7, doi:10.3390/ijms17071000, ISSN 1661-6596, page 1000, XP093016595
OPPOSITION- Laura Ordovás, Boon Ruben, Pistoni Mariaelena, Chen Yemiao, Sambathkumar Rangarajan, Helsen Nicky, Vanhove Jolien, Berckmans Pieter, Cai Qing, Vanuytsel Kim, Raitano Susanna, Verfaillie Catherine M., "Rapid and Efficient Generation of Recombinant Human Pluripotent Stem Cells by Recombinase-mediated Cassette Exchange in the <em>AAVS1</em> Locus", Journal of Visualized Experiments, vol. e54718, no. 117, doi:10.3791/54718, pages 1 - 9, XP055506042
OPPOSITION- Jihong Chen, Qiao Li, "Retinoic Acid Receptor Signaling in the Differentiation of Pluripotent Stem Cells into Skeletal Muscle Lineage", Pluripotent Stem Cells - From the Bench to the Clinic, InTech , (20160720), doi:10.5772/62819, ISBN 978-953-51-2472-6, XP055441127
OPPOSITION- Xu Tao, Zhang Mingliang, Laurent Timothy, Xie Min, Ding Sheng, "Concise Review: Chemical Approaches for Modulating Lineage-Specific Stem Cells and Progenitors", Stem Cells Translational Medicine, AlphaMed Press, Inc., US, US , (20130501), vol. 2, no. 5, doi:10.5966/sctm.2012-0172, ISSN 2157-6564, pages 355 - 361, XP093198262
OTHER- Anonymous, "Introduction to Tet expression systems", eNews, (20150401), pages 1 - 2, URL: https://www.jax.org/news-and-insights/2015/april/introduction-to-tet-expression-systems#, XP055713341
OTHER- IACOVINO et al., "Inducible cassette exchange: a rapid and efficient system enabling conditional gene expression in embryonic stem and primary cells /NIH Author Manuscript/", Stem Cells, (20111000), vol. 29, no. 10, pages 1580 - 1588, XP055713343
OTHER- SADELAIN et al., "Safe harbours for the integration of new DNA in the human genome", Nat Rev Cancer, (20120000), vol. 12, pages 51 - 58, XP055018235
OTHER- Ying Pei, Sierra Guadalupe, Sivapatham Renuka, Swistowski Andrzej, Rao Mahendra S., Zeng Xianmin, "A platform for rapid generation of single and multiplexed reporters in human iPSC lines", Sci Rep, (20150317), vol. 5, pages 1 - 10, XP055713337

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