Compositions And Methods For Synthesizing 5'-Capped Rnas - EP3954225

The patent EP3954225 was granted to Trilink Biotechnologies on Nov 22, 2023. The application was originally filed on Sep 20, 2016 under application number EP21173142A. The patent is currently recorded with a legal status of "Granted And Under Opposition".

EP3954225

TRILINK BIOTECHNOLOGIES
Application Number
EP21173142A
Filing Date
Sep 20, 2016
Status
Granted And Under Opposition
Oct 20, 2023
Grant Date
Nov 22, 2023
External Links
Slate, Register, Google Patents

Patent Summary

Patent Family

Patent Family

Patent Oppositions

Patent oppositions filed by competitors challenge the validity of a granted patent. These oppositions are typically based on claims of prior art, lack of novelty, or non-obviousness. They are a key part of the process for determining a patent's strength and enforceability.

CompanyOpposition DateOpposition Status

Get instant alerts for new oppositions and patent status changes

COHAUSZ & FLORACK PATENT UND RECHTSANWALTE PARTNERSCHAFTSGESELLSCHAFT MBBAug 22, 2024ADMISSIBLE

Patent Citations (10) New

Patent citations refer to prior patents cited during different phases such as opposition or international search.

Citation PhasePublication NumberPublication Link
DESCRIPTIONUS2007281308
DESCRIPTIONUS2012156751
DESCRIPTIONUS6762298
DESCRIPTIONUS7074596
DESCRIPTIONUS8344118
OPPOSITIONWO2008016473
OPPOSITIONWO2009058911
OPPOSITIONWO2022006368
SEARCHWO2008016473
SEARCHWO2009058911

Non-Patent Literature (NPL) Citations (136) New

NPL citations refer to non-patent references such as research papers, articles, or other publications cited during examination or opposition phases.

Citation PhaseReference TextLink
DESCRIPTION- Analytical Chemistry, (20090000), vol. 81, pages 4955 - 4962-
DESCRIPTION- Ann. Rev. Biochem., (19990000), vol. 68, pages 913 - 963-
DESCRIPTION- Biochemistry, (19800000), vol. 19, pages 3245 - 3253-
DESCRIPTION- Biochemistry, (19850000), vol. 24, pages 5716 - 5723-
DESCRIPTION- Biochemistry, (19880000), vol. 27, pages 3966 - 3974-
DESCRIPTION- Bioconjug. Chem., (19990000), vol. 10371, page 378-
DESCRIPTION- Bioorganic & Medicinal Chemistry, (20130000), vol. 21, pages 7921 - 7928-
DESCRIPTION- Bioorgan. Med. Chem. Letters, (20070000), vol. 17, pages 5295 - 5299-
DESCRIPTION- Bioorg. Med. Chem. Lett., (20070000), vol. 17, pages 5295 - 5299-
DESCRIPTION- Cell, (19760000), vol. 9, pages 645 - 653-
DESCRIPTION- Cell, (19790000), vol. 16, pages 815 - 25-
DESCRIPTION- Cell, (19850000), vol. 40, pages 223 - 24-
DESCRIPTION- Collect. Symp. Ser., (20080000), vol. 10, pages 259 - 263-
DESCRIPTION- CREY-DESBIOLLES, C., Nucleic Acids Res., (20050000), vol. 33, pages 2620 - 2628-
DESCRIPTION- EMBO, (20020000), vol. 21, pages 2757 - 2768-
DESCRIPTION- Federation of Experimental Biologists Society Letter, (19780000), vol. 96, pages 1 - 11-
DESCRIPTION- HILL, F., Proc Natl Acad. Sci. USA, (19980000), vol. 95, pages 4258 - 4263-
DESCRIPTION- ISHICAWA et al., Nucleic Acids Symposium, (20090000), vol. 53, page 129-
DESCRIPTION- ISHICAWA et al., Nucleic Acids Symposium Series, (20090000), vol. 53, no. 53, page 129-
DESCRIPTION- J. Biol. Chem., (19730000), vol. 248, pages 2235 - 2244-
DESCRIPTION- J. Biol. Chem., (19750000), vol. 250, page 9322-
DESCRIPTION- J. Biol. Chem., (19780000), vol. 253, pages 5228 - 5231-
DESCRIPTION- J. Biol. Chem., (19800000), vol. 255, page 11588-
DESCRIPTION- J. Biol. Chem., (19920000), vol. 267, page 16430-
DESCRIPTION- J. Biol. Chem., (19940000), vol. 269, pages 14974 - 14981-
DESCRIPTION- J. Biol. Chem., (19960000), vol. 271, pages 11936 - 11944-
DESCRIPTION- J. Biol. Chem., (19990000), vol. 274, pages 30337 - 3040-
DESCRIPTION- J. Biol. Chem., (20030000), vol. 278, pages 2819 - 2823-
DESCRIPTION- J. Gen. Virol., (20100000), vol. 91, pages 112 - 121-
DESCRIPTION- J. Mol. Biol., (19850000), vol. 183, pages 165 - 177-
DESCRIPTION- J. Mol. Biol., (20070000), vol. 370, pages 256 - 268-
DESCRIPTION- J. Org. Chem., (19990000), vol. 64, pages 5836 - 5840-
DESCRIPTION- LEBEDEV, A.V. et al., E., Perspect. Drug Discov. Des.,, (19960000), vol. 4, pages 17 - 40-
DESCRIPTION- LOAKES, D., Nucleic Acids Res., (20010000), vol. 29, pages 2437 - 2447-
DESCRIPTION- Meth. Enzymol.,, (19890000), vol. 180, pages 42 - 50-
DESCRIPTION- Methods Enzymol., (19890000), vol. 180, pages 51 - 62-
DESCRIPTION- Methods Mol Bio, (19970000), vol. 1, no. 74, pages 99 - 110-
DESCRIPTION- Methods Mol. Biol, (19970000), vol. 74, pages 99 - 110-
DESCRIPTION- Methods Mol. Biol., (20040000), vol. 252, pages 9 - 17-
DESCRIPTION- Microbiol. Rev., (19800000), vol. 44, page 175-
DESCRIPTION- Mol. Cell Biol., (19870000), vol. 7, pages 3371 - 3379-
DESCRIPTION- Nature, (19750000), vol. 255, pages 33 - 37-
DESCRIPTION- Nature, (19770000), vol. 266, page 235-
DESCRIPTION- Nucl. Acids Res., (19820000), vol. 10, pages 6353 - 6362-
DESCRIPTION- Nucl. Acids Res., (19840000), vol. 12, pages 7035 - 7056-
DESCRIPTION- Nucl. Acids Res., (19940000), vol. 22, pages 2430 - 2431-
DESCRIPTION- Nucleic Acid Research, (19870000), vol. 15, pages 8783 - 8798-
DESCRIPTION- Nucleic Acid Research, (20150000), vol. 43, pages 482 - 492-
DESCRIPTION- Nucleic Acids Res., (19810000), vol. 9, pages 31 - 45-
DESCRIPTION- Nucleic Acids Res., (19870000), vol. 15, pages 8783 - 8798-
DESCRIPTION- Nucleic Acids Res., (19980000), vol. 26, page 3208-
DESCRIPTION- Nucleic Acids Res., (20080000), vol. 36, page e131-
DESCRIPTION- Nucleic Acids Res., (20110000), vol. 39, page 4756-
DESCRIPTION- Nucleic Acids Research, (19930000), vol. 21, pages 1097 - 1101-
DESCRIPTION- Nucleic Acids Research, (20090000), vol. 37, pages 1925 - 1935-
DESCRIPTION- Nucleoside Nucleotides & Nucleic acids, (20060000), vol. 25, pages 337 - 340-
DESCRIPTION- Nucleosides, Nucleotides, and Nucleic Acids, (20050000), vol. 24, pages 1131 - 1134-
DESCRIPTION- Nucleosides, Nucleotides, and Nucleic Acids, (20060000), vol. 25, no. 3, pages 307 - 340-
DESCRIPTION- OHKUBO et al., Org. Letters, (20130000), vol. 15, pages 4386 - 4389-
DESCRIPTION- Org. Biomol. Chem., (20050000), vol. 3, pages 3851 - 3868-
DESCRIPTION- PITULLE, C et al., Gene, (19920000), vol. 112, pages 101 - 105-
DESCRIPTION- PREPARATA, FPOLIVER, JS, J. Comput. Biol., (20040000), pages 753 - 765-
DESCRIPTION- Proc. Natl. Acad. Sci. USA, (19750000), vol. 72, pages 1189 - 1193-
DESCRIPTION- Proc. Natl. Acad. Sci. USA, (19800000), vol. 77, pages 3952 - 3956-
DESCRIPTION- Proc. Natl. Acad. Sci. U. S. A., (19860000), vol. 83, pages 3614 - 3618-
DESCRIPTION- Proc. Natl. Acad. Sci. USA, (19970000), vol. 94, page 9573-
DESCRIPTION- Prog. Nuc. Acid Res., (19880000), vol. 35, pages 173 - 207-
DESCRIPTION- Prog. Nucleic Acid Res. Mol. Biol., (19950000), vol. 50, pages 101 - 129-
DESCRIPTION- Prog. Nucleic Acid Res. Mol. Biol., (20010000), vol. 66, pages 1 - 40-
DESCRIPTION- RNA, (19950000), vol. 1, pages 957 - 967-
DESCRIPTION- RNA, (20010000), vol. 7, pages 1486 - 1495-
DESCRIPTION- RNA, (20030000), vol. 9, pages 1108 - 1122-
DESCRIPTION- RNA, (20080000), vol. 14, pages 1119 - 1131-
DESCRIPTION- STEC, W.J. et al., Chem. Int. Ed. Engl., (19940000), vol. 33, pages 709 - 722-
DESCRIPTION- WALDNER et al., Bioorg. Med. Chem. Letters, (19960000), vol. 6, pages 2363 - 2366-
EXAMINATION- M. Ishikawa ET AL, "Preparation of eukaryotic mRNA having differently methylated adenosine at the 5'-terminus and the effect of the methyl group in translation", Nucleic Acids Symposium Series, GB, (20090901), vol. 53, no. 1, doi:10.1093/nass/nrp065, ISSN 0261-3166, pages 129 - 130, XP055331388
OPPOSITION- Alberts Bruce, Johnson Alexander, Lewis Julian, Raff Martin, Roberts Keith, Walter Peter, "Passage; Chapter 6 - How Cells Read the Genome: From DNA to Protein", Alberts Bruce, Johnson Alexander, Lewis Julian, Raff Martin, Roberts Keith, Walter Peter, ALBERTS B. ET AL., MOLECULAR BIOLOGY OF THE CELL, NEW YORK , GARLAND SCIENCE , (20080101), pages 331 - 339, ISBN 978-0-8153-4105-5, XP055896354-
OPPOSITION- Anonymous, "Luciferase SP6/T7 Control DNAs", Promega Products - Protein Expression - Cell-Free Protein Expression, Promega, pages 1 - 1, Promega Products - Protein Expression - Cell-Free Protein Expression, URL: https://nld.promega.com/products/protein-expression/cell-free-protein-expression/luciferase-sp6_t7-control-dnas/?catNum=L4821, XP093320023-
OPPOSITION- Anonymous, "mMESSAGE mMACHINE® T7 Ultra Kit - Synthesis of Translation Enhanced Capped Transcripts", User Guide, ambion®, (20140221), no. AM1345, pages 1 - 36, XP055897133-
OPPOSITION- Anonymous, "pGEM®-4Z Vector - instructions for use of product P2161", Technical Bulletin, Promega, (20090501), pages 1 - 7, XP055896367-
OPPOSITION- D23a - Annex A (CV) referred to in Experimental Report D23 on file-
OPPOSITION- D23b - Annex B (technical note) referred to in Experimental Report D23 already on file-
OPPOSITION- D24 - Experimental report “Capping Efficiency Comparison: -1+1 vs. +1+2 hybridization”-
OPPOSITION- D25 - Supplemental experimental report to D24 “Capping Efficiency Comparison: -1+1 vs. +1+2 hybridization”-
OPPOSITION- D26 - Expert Declaration by Professor Jeffery Coller, plus annexed CV.-
OPPOSITION- D28 - Post-filed data showing improved translation efficiency when R3 is O-alkyl instead of OH. Opposition to European Patent No. EP 3954225 B1 Page 3 Response by Patent Proprietor 03 January 2025-
OPPOSITION- D29 - Post-filed data showing improved capping efficiency and improved translation efficiency for compounds having adenine at B1 and uracil at B10-
OPPOSITION- D30 - Declaration of Dr Chanfeng Zhao-
OPPOSITION- D37a - Communication “Promega T7 Control DNA L4821” from Promega representative providing pLucT7 plasmid sequence dated 14 March 2025-
OPPOSITION- D37 - Sequence of Promega T7 Luc plasmid provided by Promega-
OPPOSITION- D44 - Declaration of Dr. Brian Liau, the author of D23 and principal scientist at GDMC responsible for the experiments reported in D23-
OPPOSITION- D45 - Experimental report “aborted RNA shortmers in IVT reactions”-
OPPOSITION- D46 - Experimental report “translational efficiency”-
OPPOSITION- Gruegelsiepe Heike, Schon Astrid, Kirsebom Leif A., Hartmann Roland K., "Chapter 1 - Enzymatic RNA Synthesis, Ligation and Modification", Handbook of RNA Biochemistry, Part I - RNA Synthesis, Wiley-VCH, (20050101), pages 3 - 21, ISBN 3-527-30826-1, XP055896409-
OPPOSITION- Harvey Lodish, "Chapter 4.2 Transcription of Protein-Coding Genes and Formation of Functional mRNA", Molecular cell biology (6th ed.)., W.H. Freeman, (20080101), pages 120 - 126, ISBN 0-7167-7601-4, XP009563340-
OPPOSITION- Liau Brian, "mRNA Capping Analysis Report - dinucleotide vs. trinuc/eotide cap analogs", Genetic Design & Manufacturing, (20210101), pages 1 - 34, XP093098425-
OPPOSITION- Pasquinelli Amy E, Dahlberg J E, Lund E, "Reverse 5' caps in RNAs made in vitro by phage RNA polymerases", RNA, COLD SPRING HARBOR LABORATORY PRESS, US, US , (19951101), vol. 1, no. 9, ISSN 1355-8382, pages 957 - 967, XP093061904-
OPPOSITION- Dudley Ed, Bond Liz, "Mass spectrometry analysis of nucleosides and nucleotides : MASS SPECTROMETRY OF NUCLEOSIDES AND NUCLEOTIDES", MASS SPECTROMETRY REVIEWS., JOHN WILEY AND SONS, NEW YORK, NY., US, US , (20140701), vol. 33, no. 4, doi:10.1002/mas.21388, ISSN 0277-7037, pages 302 - 331, XP093165178
OPPOSITION- Beckert Bertrand, Masquida Benoît, "Synthesis of RNA by In Vitro Transcription : Methods and Protocols", RNA : Methods and Protocols : Methods and Protocols, Totowa, NJ, Humana Press, (20110101), vol. 703, pages 29 - 41, doi:10.1007/978-1-59745-248-9_3, ISSN 1064-3745, ISBN 978-1-59745-248-9, XP093153524
OPPOSITION- Krupp, G., "RNA synthesis: strategies for the use of bacteriophage RNA polymerases", Gene, Elsevier Amsterdam, NL, NL , (19881210), vol. 72, no. 1-2, doi:10.1016/0378-1119(88)90129-1, ISSN 0378-1119, pages 75 - 89, XP025722560
OPPOSITION- Tuytten, R. ; Lemiere, F. ; Van Dongen, W. ; Esmans, E.L. ; Witters, E. ; Herrebout, W. ; Van Der Veken, B. ; Dudley, E. ; Newton, R.P., "Intriguing Mass Spectrometric Behavior of Guanosine Under Low Energy Collision-Induced Dissociation: H"2O Adduct Formation and Gas-Phase Reactions in the Collision Cell", Journal of the American society for mass spectrometry, Elsevier science INC, US, US , (20050801), vol. 16, no. 8, doi:10.1016/j.jasms.2005.03.026, ISSN 1044-0305, pages 1291 - 1304, XP027790353
OPPOSITION- Kennedy, W.P. Momand, J.R. Yin, Y.W., "Mechanism for De Novo RNA Synthesis and Initiating Nucleotide Specificity by T7 RNA Polymerase", Journal of Molecular Biology, Academic Press, United Kingdom, United Kingdom , (20070601), vol. 370, no. 2, doi:10.1016/j.jmb.2007.03.041, ISSN 0022-2836, pages 256 - 268, XP022103110
OPPOSITION- Sriram Vaidyanathan, Azizian Krist T., Haque A.K.M. Ashiqul, Henderson Jordana M., Hendel Ayal, Shore Sabrina, Antony Justin S., Hogrefe Richard I., Kormann Michael S.D., Porteus Matthew H., Mccaffrey Anton P., "Uridine Depletion and Chemical Modification Increase Cas9 mRNA Activity and Reduce Immunogenicity without HPLC Purification", Molecular Therapy-Nucleic Acids, Cell Press, US, US , (20180901), vol. 12, doi:10.1016/j.omtn.2018.06.010, ISSN 2162-2531, pages 530 - 542, XP055531206
OPPOSITION- Pierre Fechter; Joëlle Rudinger; Richard Giegé; Anne Théobald-Dietrich, "Ribozyme processed tRNA transcripts with unfriendly internal promoter for T7 RNA polymerase: production and activity", FEBS Letters, ELSEVIER, AMSTERDAM., NL, NL , (19981020), vol. 436, no. 1, doi:10.1016/S0014-5793(98)01096-5, ISSN 0014-5793, pages 99 - 103, XP071237558
OPPOSITION- Nam Sang-Chul, Changwon Kang, "Transcription Initiation Site Selection and Abortive Initiation Cycling of Phage SP6 RNA Polymerase", Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, US, US , (19881205), vol. 263, no. 24, doi:10.1016/S0021-9258(19)81332-7, ISSN 0021-9258, pages 18123 - 18127, XP055896394
OPPOSITION- Ishikawa, M. Hirao, I. Yokoyama, S., "Synthesis of 3-(2-deoxy-@b-d-ribofuranosyl)pyridin-2-one and 2-amino-6-(N,N-dimethylamino)-9-(2-deoxy-@b-d-ribofuranosyl)purine derivatives for an unnatural base pair", Tetrahedron Letters, Elsevier, AMSTERDAM, NL, AMSTERDAM, NL, (20000501), vol. 41, no. 20, doi:10.1016/S0040-4039(00)00520-7, ISSN 0040-4039, pages 3931 - 3934, XP004203291
OPPOSITION- FUKUOKA K., ET AL., "ONE-POT REACTION FOR THE SYNTHESIS OF M7G5'PPPG AND M7G5'PPPA BY USING AN ACTIVATABLE BIFUNCTIONAL PHOSPHORYLATING REAGENT.", Tetrahedron Letters, Elsevier, AMSTERDAM, NL, AMSTERDAM, NL, (19940214), vol. 35., no. 07., doi:10.1016/S0040-4039(00)79966-7, ISSN 0040-4039, pages 1063 - 1066., XP000608145
OPPOSITION- Ichiro Hirao, Michiko Kimoto, Shun-ichi Yamakage, Masahide Ishikawa, Jun Kikuchi, Shigeyuki Yokoyama, "A unique unnatural base pair between a C analogue, pseudoisocytosine and an A analogue, 6-methoxypurine, in replication", Bioorganic & Medicinal Chemistry Letters, ELSEVIER, Amsterdam NL, Amsterdam NL , (20020520), vol. 12, no. 5A, doi:10.1016/S0960-894X(02)00184-1, ISSN 0960-894X, pages 1391 - 1393, XP002974102
OPPOSITION- Stepinski Janusz, Waddell Clint, Stolarski Ryszard, Darzynkiewicz Edward, Rhoads Robert E, "Synthesis and properties of mRNAs containing the novel "anti-reverse" cap analogs 7-methyl(39-O-methyl)GpppG and 7-methyl(39-deoxy)GpppG", RNA, COLD SPRING HARBOR LABORATORY PRESS, US, US , (20011001), vol. 7, no. 10, doi:10.1017.S1355838201014078, ISSN 1355-8382, pages 1486 - 1495, XP093209922
OPPOSITION- Nakayama Hiroshi, Yamauchi Yoshio, Nobe Yuko, Sato Ko, Takahashi Nobuhiro, Shalev-Benami Moran, Isobe Toshiaki, Taoka Masato, "Method for Direct Mass-Spectrometry-Based Identification of Monomethylated RNA Nucleoside Positional Isomers and Its Application to the Analysis of Leishmania rRNA", Analytical Chemistry, American Chemical Society, (20191217), vol. 91, no. 24, doi:10.1021/acs.analchem.9b03735, ISSN 0003-2700, pages 15634 - 15643, XP093165179
OPPOSITION- Diane Imburgio, Minqing Rong, Kaiyu Ma, William T. Mcallister, "Studies of Promoter Recognition and Start Site Selection by T7 RNA Polymerase Using a Comprehensive Collection of Promoter Variants †", Biochemistry, American Chemical Society, (20000801), vol. 39, no. 34, doi:10.1021/bi000365w, ISSN 00062960, pages 10419 - 10430, XP055125483
OPPOSITION- AXELROD V. D., KRAMER F. R., "Transcription from bacteriophage T7 and SP6 RNA polymerase promoters in the presence of 3'-deoxyribonucleoside 5'-triphosphate chain terminators", Biochemistry, American Chemical Society, (19851001), vol. 24, no. 21, doi:10.1021/bi00342a005, ISSN 0006-2960, pages 5716 - 5723, XP002912288
OPPOSITION- Johnson Patrick A., Gaspar Mariafrancis A., Levicky Rastislav, "Polymer-Anchored DNA Gene Monolayers", Journal of the American Chemical Society, ACS Publications, (20040801), vol. 126, no. 32, doi:10.1021/ja048415y, ISSN 0002-7863, pages 9910 - 9911, XP093320020
OPPOSITION- Johnson Patrick A., Gaspar Mariafrancis A., Levicky Rastislav, "Supporting Information: Polymer-Anchored DNA Gene Monolayers", Journal of the American Chemical Society, ACS Publications, (20040801), vol. 126, no. 32, doi:10.1021/ja048415y, ISSN 0002-7863, pages 1 - 5, XP093320019
OPPOSITION- Anilkumar R. Kore, Muthian Shanmugasundaram, Irudaya Charles, Alexander V. Vlassov, Timothy J. Barta, "Locked Nucleic Acid (LNA)-Modified Dinucleotide mRNA Cap Analogue: Synthesis, Enzymatic Incorporation, and Utilization", Journal of the American Chemical Society, ACS Publications, (20090513), vol. 131, no. 18, doi:10.1021/ja901655p, ISSN 0002-7863, pages 6364 - 6365, XP055088810
OPPOSITION- KUHN A N, ET AL, "Phosphorothioate cap analogs increase stability and translational efficiency of RNA vaccines in immature dendritic cells and induce superior immune responses in vivo", Gene Therapy, Nature Publishing Group, London, GB, GB , (20100801), vol. 17, no. 8, doi:10.1038/GT.2010.52, ISSN 0969-7128, pages 961 - 971, XP002607370
OPPOSITION- T. OHTSUKI ET AL., "Unnatural base pairs for specific transcription", Proceedings of the National Academy of Sciences (PNAS), National Academy of Sciences, (20010424), vol. 98, no. 9, doi:10.1073/PNAS.091532698, ISSN 0027-8424, pages 4922 - 4925, XP002546129
OPPOSITION- Kathleen E. Mcginness, Gerald F. Joyce, "Substitution of Ribonucleotides in the T7 RNA Polymerase Promoter Element", Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, US, US , (20020125), vol. 277, no. 4, doi:10.1074/jbc.M108820200, ISSN 0021-9258, pages 2987 - 2991, XP055736077
OPPOSITION- Iaroslav Kuzmine, Philip A. Gottlieb, Craig T. Martin, "Binding of the Priming Nucleotide in the Initiation of Transcription by T7 RNA Polymerase", Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, US, US , (20030131), vol. 278, no. 5, doi:10.1074/jbc.M208405200, ISSN 0021-9258, pages 2819 - 2823, XP055693453
OPPOSITION- GRUDZIEN EWA ET AL, "Differential inhibition of mRNA degradation pathways by novel cap analogs", Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, US, US , (20060101), vol. 281, no. 4, doi:10.1074/jbc.M509121200, ISSN 0021-9258, pages 1857 - 1867, XP002452226
OPPOSITION- Kamzolova S.G., Beskaravainy P.M., Osypov A.A., Dzhelyadin T.R., Temlyakova E.A., Sorokin A.A., "Electrostatic map of T7 DNA: comparative analysis of functional and electrostatic properties of T7 RNA polymerase-specific promoters", JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, ADENINE PRESS, NEW YORK, NY, US, US , (20140803), vol. 32, no. 8, doi:10.1080/07391102.2013.819298, ISSN 0739-1102, pages 1184 - 1192, XP093093281
OPPOSITION- Yutaka Suzuki; Hirotoshi Taira; Tatsuhiko Tsunoda; Junko Mizushima‐Sugano; Jun Sese; Hiroko Hata; Toshio Ota; Takao Isogai; Toshihiro Tanaka; Shinichi Morishita; Kousaku Okubo; Yoshiyuki Sakaki; Yusuke Nakamura; Akira Suyama; Sumio Sugano, "Diverse transcriptional initiation revealed by fine, large‐scale mapping of mRNA start sites", EMBO REPORTS, NATURE PUBLISHING GROUP, LONDON, GB, GB , (20010501), vol. 2, no. 5, doi:10.1093/embo-reports/kve085, ISSN 1469-221X, pages 388 - 393, XP072228544
OPPOSITION- ARNAUD-BARBE N ET AL, "Transcription of RNA templates by T7 RNA polymerase.", Nucleic Acids Research, Information Retrieval Ltd., England, England, (19980801), vol. 26, no. 15, doi:10.1093/nar/26.15.3550, ISSN 0305-1048, pages 3550 - 3554, XP002305270
OPPOSITION- F. Peyrane, B. Selisko, E. Decroly, J. J. Vasseur, D. Benarroch, B. Canard, K. Alvarez, "High-yield production of short GpppA- and 7MeGpppA-capped RNAs and HPLC-monitoring of methyltransfer reactions at the guanine-N7 and adenosine-2'O positions.", Nucleic Acids research, Oxford University Press, GB, GB , (20070101), vol. 35, no. 4, doi:10.1093/NAR/GKL1119, ISSN 1362-4962, pages E26.1 - E26.11, XP002631670
OPPOSITION- Tricia M. Coleman, Guocan Wang, Faqing Huang, "Superior 5' homogeneity of RNA from ATP-initiated transcription under the T7  2.5 promoter", Nucleic Acids Research, Oxford University Press, GB, GB , (20040101), vol. 32, no. 1, doi:10.1093/nar/gnh007, ISSN 0305-1048, pages 14e - 4, XP055718310
OPPOSITION- ISHIKAWA M, HIRAO I, YOKOYAMA S, "CHEMICAL SYNTHESIS OF NOVEL BASE PAIRS AND THEIR ENZYMATIC INCORPORATION INTO DNA", Nucleic Acids Symposium Series, Oxford University Press, GB, GB , (19991101), vol. 42, no. 1, doi:10.1093/nass/42.1.125, ISSN 0261-3166, pages 125 - 126, XP001089633
OPPOSITION- M. Ishikawa, R. Murai, H. Hagiwara, T. Hoshino, K. Suyama, "Preparation of eukaryotic mRNA having differently methylated adenosine at the 5'-terminus and the effect of the methyl group in translation", Nucleic Acids Symposium Series, Oxford University Press, GB, GB , (20090901), vol. 53, no. 1, doi:10.1093/nass/nrp065, ISSN 0261-3166, pages 129 - 130, XP055331388
OPPOSITION- Joubert Pascale, Pautigny Carine, Madelaine Marie-Françoise, Rasschaert Denis, "Identification of a new cleavage site of the 3C-like protease of rabbit haemorrhagic disease virus", MICROBIOLOGY, Society for General Microbiology, Reading, Reading , (20000201), vol. 81, no. 2, doi:10.1099/0022-1317-81-2-481, ISSN 1350-0872, pages 481 - 488, XP093320015
OPPOSITION- Ray Debashish, Shah Aaloki, Tilgner Mark, Guo Yi, Zhao Yiwei, Dong Hongping, Deas Tia S., Zhou Yangsheng, Li Hongmin, Shi Pei-Yong, "West Nile Virus 5′-Cap Structure Is Formed by Sequential Guanine N-7 and Ribose 2′-O Methylations by Nonstructural Protein 5", Journal of Virology, THE AMERICAN SOCIETY FOR MICROBIOLOGY, US, US , (20060901), vol. 80, no. 17, doi:10.1128/JVI.00814-06, ISSN 0022-538X, pages 8362 - 8370, XP055897121
OPPOSITION- Banerjee Amiya K, "5'-Terminal Cap Structure in Eucaryotic Messenger Ribonucleic Acids", MICROBIOLOGICAL REVIEWS, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, DC, US, US , (19800601), vol. 44, no. 2, doi:10.1128/mr.44.2.175-205.1980, ISSN 0146-0749, pages 175 - 205, XP055897139
OPPOSITION- JEMIELITY JACEK, ET AL, "Novel "anti-reverse" cap analogs with superior translational properties", RNA, COLD SPRING HARBOR LABORATORY PRESS, US, US , (20030101), vol. 9, no. 9, doi:10.1261/rna.5430403, ISSN 1355-8382, pages 1108 - 1122, XP002466761
OPPOSITION- Marcin Ziemniak, Malwina Strenkowska, Joanna Kowalska, Jacek Jemielity, "Potential therapeutic applications of RNA cap analogs", Future Medicinal Chemistry, Future Science, England, England, (20130601), vol. 5, no. 10, doi:10.4155/fmc.13.96, ISSN 1756-8919, pages 1141 - 1172, XP055573135
SEARCH- ROBERT E. SMITH ET AL, "A Unique Class of Compound, Guanosine-Nucleoside Tetraphosphate G(5')pppp(5')N, Synthesized during the in Vitro Transcription of Cytoplasmic Polyhedrosis Virus of Bombyx mori STRUCTURAL DETERMINATION AND MECHANISM OF FORMATION*", THE JOURNAL OF BIOLOGICAL CHEMISTRY, (19820101), vol. 257, no. 1, pages 485 - 494, XP055331398 [Y] 1-15 * the whole document * * abstract *-
SEARCH- HIROAKI SAWAI ET AL, "Synthesis and Reactions of Nucleoside 5'-Diphosphate Imidazolide. A Nonenzymatic Capping Agent for 5'-Monophosphorylated Oligoribonucleotides in Aqueous Solution", JOURNAL OF ORGANIC CHEMISTRY, (19990801), vol. 64, no. 16, doi:10.1021/jo990286u, ISSN 0022-3263, pages 5836 - 5840, XP055331109 [Y] 1-15 * the whole document * * Materials and methods * * Scheme 2 *
SEARCH- I. I. KOUKHAREVA ET AL, "Chemical Route to the Capped RNAs", NUCLEOSIDES, NUCLEOTIDES AND NUCLEIC ACIDS., US, (20040101), vol. 23, no. 10, doi:10.1081/NCN-200031492, ISSN 1525-7770, pages 1667 - 1680, XP055553747 [Y] 1-15 * the whole document * * table 1 * * Results *
SEARCH- Y. THILLIER ET AL, "Synthesis of 5' cap-0 and cap-1 RNAs using solid-phase chemistry coupled with enzymatic methylation by human (guanine-N7)-methyl transferase", RNA, US, (20120214), vol. 18, no. 4, doi:10.1261/rna.030932.111, ISSN 1355-8382, pages 856 - 868, XP055553150 [Y] 1-15 * the whole document * * figure 1 * * Results and Discussion *

Download Citation Report

Get a free citation report including examiner, opposition, and international search citations.

Get Citation Report

Dossier Documents

The dossier documents provide a comprehensive record of the patent’s prosecution history - including filings, correspondence, and decisions made by patent offices - and are crucial for understanding the patent’s legal journey and any challenges it may have faced during examination.

  • Date

    Description

  • Get instant alerts for new documents