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Methods And Systems For Amplification In Complex Samples

Patent No. EP3443124 (titled "Methods And Systems For Amplification In Complex Samples") was filed by T Biosystems on Apr 12, 2017. The application was issued on Sep 3, 2025.

Patent Summary

A method for amplifying pathogen-characteristic target nucleic acids in a 0.2 mL to 5 mL whole blood sample containing subject-derived cells or debris comprises: (a) lysing cells to form a lysate; (b) adding a buffer solution with a moderately alkaline pH (8.5 to 9.5) at ambient temperature to create a reaction mixture; (c) heating the mixture to denature it, followed by centrifugation; (d) adding a thermostable nucleic acid polymerase to the denatured mixture; and (e) performing polymerase chain reaction (PCR) to produce an amplicon. This process minimizes inhibition from heme compounds, IgG, and excess host DNA without extensive purification. Optional steps include adding dNTPs, magnesium, and primers, and detecting amplicons via optical, electrochemical, or NMR-based magnetic particle aggregation.

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Patent Oppositions (3)

Patent oppositions filed by competitors challenge the validity of a granted patent. These oppositions are typically based on claims of prior art, lack of novelty, or non-obviousness. They are a key part of the process for determining a patent's strength and enforceability.

CompanyOpposition DateRepresentative
ROTHMay 17, 2023KONIG SZYNKA TILMANN VON RENESSE PATENTANWALTE PARTNERSCHAFT MBB DUSSELDORF
BIOMRIEUXMay 16, 2023CABINET BEAU DE LOMNIE
BIOMERIEUXMay 16, 2023ATOUT PI LAPLACE

Dossier Documents

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EP3443124

T BIOSYSTEMS
Application Number
EP17783057A
Filing Date
Apr 12, 2017
Status
Patent Maintained As Amended
Aug 1, 2025
Publication Date
Sep 3, 2025
External Links
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