Patent No. EP3686266 (titled "Synbiotic Surface Treatment Composition") was filed by Chrisal on Jan 23, 2019. The application was issued on Oct 23, 2024.
A method for treating abiotic surfaces—including wood, paper, leather, rubber, textile, or mixtures thereof—utilizes a surface treatment composition comprising a prebiotic component and spores of non-pathogenic *Bacillus* species. The probiotic component consists of a mixture of *Bacillus subtilis*, *B. licheniformis*, *B. megaterium*, and *B. amyloliquefaciens*, where each species constitutes 10% to 70% of the total spore count. The prebiotic component comprises at least one of inulin, fructo-oligosaccharide, or galacto-oligosaccharide. Treatment is performed via cleaning, spraying, ultrasound fogging, painting, or water dosing to reduce pathogenic microorganisms. The composition may be formulated as a detergent or liquid, and the spores and prebiotics may be encapsulated in brittle-shelled microcapsules containing a non-aqueous solution to accelerate probiotic activity and enhance biofilm removal.

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