Patent No. EP3692360 (titled "Methods And Systems For 3D Structure Estimation Using Non-Uniform Refinement") was filed by The Governing Council OF The University OF Toronto on Oct 5, 2018. The application was issued on Sep 6, 2023.
Systems and methods for 3D structure estimation from electron Cryo-microscopy images using non-uniform refinement. The approach involves iteratively refining 3D maps by splitting the particle images into half-sets, determining local resolution estimates and filtering on each half-map, aligning particles, generating updated half-maps, and combining them to produce a final 3D map. The refinement process is optimized by minimizing the discrepancy between the two half-maps after each filtering step.

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