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Composition For Cleaving A Target Dna Comprising A Guide Rna Specific For The Target Dna And Cas Protein-Encoding Nucleic Acid Or Cas Protein, And Use Thereof

Patent No. EP4397760 (titled "Composition For Cleaving A Target Dna Comprising A Guide Rna Specific For The Target Dna And Cas Protein-Encoding Nucleic Acid Or Cas Protein, And Use Thereof") was filed by Toolgen Incorporated on Oct 23, 2013. The application was issued on Jan 21, 2026.

Patent Summary

This invention provides an in vitro or ex vivo method for cleaving target DNA, inducing targeted mutagenesis, or performing genome editing in mammalian cells. The method comprises providing a composition containing a single-chain guide RNA (sgRNA) and a Cas9 protein, and transferring this composition into the cell. Specifically, the Cas9 protein is derived from *Streptococcus pyogenes*. The sgRNA is a chimeric RNA fusing essential portions of CRISPR RNA (crRNA) and trans-activating crRNA (tracrRNA), allowing the Cas9 protein to function as a programmable RNA-guided endonuclease (RGEN). This system overcomes limitations of ZFNs and TALENs by utilizing RNA-based site specificity, enabling precise DNA cleavage and the potential for RGEN-mediated restriction fragment length polymorphism (RFLP) analysis for genotyping mutations.

UPC Cases New

Latest UPC cases involving this patent.

Case NumberFiling DatePlaintiffProceeding Type
UPC_CFI_0001830/2026May 26, 2026ToolgenInfringement

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Patent Oppositions (2)

Patent oppositions filed by competitors challenge the validity of a granted patent. These oppositions are typically based on claims of prior art, lack of novelty, or non-obviousness. They are a key part of the process for determining a patent's strength and enforceability.

CompanyOpposition DateRepresentative
VERTEX PHARMACEUTICALS INCORPORATEDJan 21, 2026CARPMAELS & RANSFORD
VOSSIUS & PARTNER PATENTANWALTE RECHTSANWALTE MBBJan 21, 2026VOSSIUS & PARTNER PATENTANWALTE RECHTSANWALTE MBB

Dossier Documents

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  • Date

    Description

EP4397760

TOOLGEN INCORPORATED
Application Number
EP24165204A
Filing Date
Oct 23, 2013
Status
Granted And Under Opposition
Dec 19, 2025
Publication Date
Jan 21, 2026
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