Composition For Cleaving A Target Dna Comprising A Guide Rna Specific For The Target Dna And Cas Protein-Encoding Nucleic Acid Or Cas Protein, And Use Thereof
Patent No. EP4397760 (titled "Composition For Cleaving A Target Dna Comprising A Guide Rna Specific For The Target Dna And Cas Protein-Encoding Nucleic Acid Or Cas Protein, And Use Thereof") was filed by Toolgen Incorporated on Oct 23, 2013. The application was issued on Jan 21, 2026.
Patent Summary
This invention provides an in vitro or ex vivo method for cleaving target DNA, inducing targeted mutagenesis, or performing genome editing in mammalian cells. The method comprises providing a composition containing a single-chain guide RNA (sgRNA) and a Cas9 protein, and transferring this composition into the cell. Specifically, the Cas9 protein is derived from *Streptococcus pyogenes*. The sgRNA is a chimeric RNA fusing essential portions of CRISPR RNA (crRNA) and trans-activating crRNA (tracrRNA), allowing the Cas9 protein to function as a programmable RNA-guided endonuclease (RGEN). This system overcomes limitations of ZFNs and TALENs by utilizing RNA-based site specificity, enabling precise DNA cleavage and the potential for RGEN-mediated restriction fragment length polymorphism (RFLP) analysis for genotyping mutations.
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EP4397760
- Application Number
- EP24165204A
- Filing Date
- Oct 23, 2013
- Status
- Granted And Under Opposition
- Dec 19, 2025
- Publication Date
- Jan 21, 2026
- External Links
- Slate, Register , Google Patents