IP Verse

Engineering And Optimization Of Improved Systems, Methods And Enzyme Compositions For Sequence Manipulation - EP3064585

The patent EP3064585 was granted to Massachusetts Institute OF Technology on Feb 5, 2020. The application was originally filed on Dec 12, 2013 under application number EP16151694A. The patent is currently recorded with a legal status of "Granted And Under Opposition".

EP3064585

MASSACHUSETTS INSTITUTE OF TECHNOLOGY
Application Number
EP16151694A
Filing Date
Dec 12, 2013
Status
Granted And Under Opposition
Jan 3, 2020
Publication Date
Feb 5, 2020
External Links
Slate, Register, Google Patents

Patent Summary

Patent Family

Patent Family

Patent Oppositions (3)

Patent oppositions filed by competitors challenge the validity of a granted patent. These oppositions are typically based on claims of prior art, lack of novelty, or non-obviousness. They are a key part of the process for determining a patent's strength and enforceability.

CompanyOpposition DateRepresentativeOpposition Status

Get instant alerts for new oppositions and patent status changes

GRUNDNov 4, 2020GRUNDADMISSIBLE
PATENT BOUTIQUESep 9, 2020-ADMISSIBLE
CRISPR THERAPEUTICSFeb 10, 2020VOSSIUS & PARTNER PATENTANWALTE RECHTSANWALTE MBBADMISSIBLE

Patent Citations (37) New

Patent citations refer to prior patents cited during different phases such as opposition or international search.

Citation PhasePublication Number
DESCRIPTIONWO1994US05700
DESCRIPTIONEP0264166
DESCRIPTIONUS4186183
DESCRIPTIONUS4217344
DESCRIPTIONUS4235871
DESCRIPTIONUS4261975
DESCRIPTIONUS4485054
DESCRIPTIONUS4501728
DESCRIPTIONUS4774085
DESCRIPTIONUS4797368
DESCRIPTIONUS4837028
DESCRIPTIONUS4873316
DESCRIPTIONUS4897355
DESCRIPTIONUS4946787
DESCRIPTIONUS5049386
DESCRIPTIONUS5173414
DESCRIPTIONUS5210015
DESCRIPTIONUS5445934
DESCRIPTIONUS6603061
DESCRIPTIONUS8404658
DESCRIPTIONWO9116024
DESCRIPTIONWO9117424
DESCRIPTIONWO9324641
DESCRIPTIONWO9639154
DESCRIPTIONWO9703211
OPPOSITIONEP2771468
OPPOSITIONEP2898075
OPPOSITIONUS2005220796
OPPOSITIONUS2015184139
OPPOSITIONWO2011146121
OPPOSITIONWO2013176772
OPPOSITIONWO2014093635
OTHEREP2771468
OTHEREP2784162
OTHEREP2896697
OTHEREP2898075
OTHEREP2931898

Non-Patent Literature (NPL) Citations (348) New

NPL citations refer to non-patent references such as research papers, articles, or other publications cited during examination or opposition phases.

Citation PhaseReference Text
DESCRIPTION- AHMAD ET AL., CANCER RES., (1992), vol. 52, pages 4817 - 4820
DESCRIPTION- ALTSCHUL ET AL., J. MOL. BIOL., (1990), pages 403 - 410
DESCRIPTION- AMRANN ET AL., GENE, (1988), vol. 69, pages 301 - 315
DESCRIPTION- ANDERSON, SCIENCE, (1992), vol. 256, pages 808 - 813
DESCRIPTION- A.R. GRUBER ET AL., CELL, (2008), vol. 106, no. 1, pages 23 - 24
DESCRIPTION- ATSCHUL ET AL., J. MOL. BIOL., (1990), pages 403 - 410
DESCRIPTION- AUSUBEL ET AL., SHORT PROTOCOLS IN MOLECULAR BIOLOGY, (1999), pages 7.58 - 7.60
DESCRIPTION- BALDARI ET AL., EMBO J., (1987), vol. 6, pages 229 - 234
DESCRIPTION- BANEIJI ET AL., CELL, (1983), vol. 33, pages 729 - 740
DESCRIPTION- BEDELL, V.M. ET AL., "In vivo genome editing using a high-efficiency TALEN system", NATURE, (2012), vol. 491, pages 114 - 133
DESCRIPTION- BEHR ET AL., BIOCONJUGATE CHEM., (1994), vol. 5, pages 382 - 389
DESCRIPTION- BHAYA, D.; DAVISON, M.; BARRANGOU, R., "CRISPR-Cas systems in bacteria and archaea: versatile small RNAs for adaptive defense and regulation", ANNU REV GENET, (2011), vol. 45, pages 273 - 297
DESCRIPTION- Biotechnology Information, NATIONAL LIBRARY OF MEDICINE
DESCRIPTION- BIRCH AM ET AL., "DGAT1 inhibitors as anti-obesity and anti-diabetic agents", CURRENT OPINION IN DRUG DISCOVERY & DEVELOPMENT, (2010), vol. 13, no. 4, pages 489 - 496
DESCRIPTION- BLAESE ET AL., CANCER GENE THER., (1995), vol. 2, pages 291 - 297
DESCRIPTION- BOBIS-WOZOWICZ, S.; OSIAK, A.; RAHMAN, S.H.; CATHOMEN, T., "Targeted genome editing in pluripotent stem cells using zinc-finger nucleases", METHODS, (2011), vol. 53, pages 339 - 346
DESCRIPTION- BOCH, J. ET AL., "Breaking the code of DNA binding specificity of TAL-type III effectors", SCIENCE, (2009), vol. 326, pages 1509 - 1512
DESCRIPTION- BOGENHAGEN, D.F.; BROWN, D.D., "Nucleotide sequences in Xenopus 5S DNA required for transcription termination", CELL, (1981), vol. 24, pages 261 - 270
DESCRIPTION- BOSHART ET AL., CELL, (1985), vol. 41, pages 521 - 530
DESCRIPTION- BUCHSCHER ET AL., J. VIROL., (1992), vol. 66, pages 2731 - 2739
DESCRIPTION- BULTMANN, S. ET AL., "Targeted transcriptional activation of silent oct4 pluripotency gene by combining designer TALEs and inhibition of epigenetic modifiers", NUCLEIC ACIDS RES, (2012), vol. 40, pages 5368 - 5377
DESCRIPTION- BYRNE; RUDDLE, PROC. NATL. ACAD. SCI. USA, (1989), vol. 86, pages 5473 - 5477
DESCRIPTION- CALAME; EATON, ADV. IMMUNOL., (1988), vol. 43, pages 235 - 275
DESCRIPTION- CAMPES; TILGHMAN, GENES DEV., (1989), vol. 3, pages 537 - 546
DESCRIPTION- CARLSON, D.F. ET AL., "Efficient TALEN-mediated gene knockout in livestock", PROC NATL ACAD SCI U S A, (2012), vol. 109, pages 17382 - 17387
DESCRIPTION- CHAN-HUI ET AL., CLINICAL IMMUNOLOGY, (2003), vol. 111, pages 162 - 174
DESCRIPTION- CHEN, F.Q. ET AL., "High-frequency genome editing using ssDNA oligonucleotides with zinc-finger nucleases", NAT METHODS, (2011), vol. 8, pages 753 - 796
DESCRIPTION- CHO, S.; GOLDBERG, M.; SON, S.; XU, Q.; YANG, F.; MEI, Y.; BOGATYREV, S.; LANGER, R.; ANDERSON, D., "Lipid-like nanoparticles for small interfering RNA delivery to endothelial cells", ADVANCED FUNCTIONAL MATERIALS, (2010), vol. 19, pages 3112 - 3118
DESCRIPTION- CHO, S.W.; KIM, S.; KIM, J.M.; KIM, J.S., "Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease", NAT BIOTECHNOL, (2013), vol. 31, pages 230 - 232
DESCRIPTION- CHRISTIAN, M. ET AL., "Targeting DNA double-strand breaks with TAL effector nucleases", GENETICS, (2010), vol. 186, pages 757 - 761
DESCRIPTION- CODON USAGE DATABASE, (20020709), URL: www.kazusa.orjp/codon
DESCRIPTION- CONG, L. ET AL., "Multiplex genome engineering using CRISPR/Cas systems", SCIENCE, (2013), vol. 339, pages 819 - 823
DESCRIPTION- CRYSTAL, SCIENCE, (1995), vol. 270, pages 404 - 410
DESCRIPTION- DELTCHEVA, E. ET AL., "CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III", NATURE, (2011), vol. 471, pages 602 - 607
DESCRIPTION- DEVEAU, H.; GARNEAU, J.E.; MOINEAU, S., "CRISPR/Cas system and its role in phage-bacteria interactions", ANNU REV MICROBIOL, (2010), vol. 64, pages 475 - 493
DESCRIPTION- DEVEREUX ET AL., NUC. ACIDS RESEARCH, (1984), vol. 12, page 387
DESCRIPTION- DEVEREUX ET AL., NUCLEIC ACIDS RESEARCH, (1984), vol. 12, page 387
DESCRIPTION- DILLON, TIBTECH, (1993), vol. 11, pages 167 - 175
DESCRIPTION- DING, Q. ET AL., "A TALEN genome-editing system for generating human stem cell-based disease models", CELL STEM CELL, (2013), vol. 12, pages 238 - 251
DESCRIPTION- DUMITRACHE ET AL., "Trex2 enables spontaneous sister chromatid exchanges without facilitating DNA double-strand break repair", GENETICS, (201108), vol. 188, no. 4, pages 787 - 797
DESCRIPTION- EDLUND ET AL., SCIENCE, (1985), vol. 230, pages 912 - 916
DESCRIPTION- EL-ANDALOUSSI S ET AL., "Exosome-mediated delivery of siRNA in vitro and in vivo", NAT PROTOC., (20121115), vol. 7, no. 12, pages 2112 - 2126
DESCRIPTION- FEMS MICROBIOL LETT., (1999), vol. 174, no. 2, pages 247 - 250
DESCRIPTION- FEMS MICROBIOL LETT., (1999), vol. 177, no. 1, pages 187 - 188
DESCRIPTION- FUCHS ET AL., "Killing of leukemic cells with a BCR/ABL fusion gene by RNA interference (RNAi", ONCOGENE, (2002), vol. 21, no. 37, pages 5716 - 5724
DESCRIPTION- GAO ET AL., GENE THERAPY, (1995), vol. 2, pages 710 - 722
DESCRIPTION- GARNEAU, J.E. ET AL., "The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA", NATURE, (2010), vol. 468, pages 67 - 71
DESCRIPTION- GASIUNAS ET AL., PROC. NATL. ACAD. SCI. USA, (2012), vol. 109, page E2579
DESCRIPTION- GASIUNAS, G.; BARRANGOU, R.; HORVATH, P.; SIKSNYS, V., "Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria", PROC NATL ACAD SCI U S A, (2012), vol. 109, pages E2579 - E2586
DESCRIPTION- Genetic Medicine, JOHNS HOPKINS
DESCRIPTION- Genetics of Epilepsy and Genetic Epilepsies, MARIANI FOUNDATION PAEDIATRIC NEUROLOGY, (2009), page 20
DESCRIPTION- GEURTS, A.M. ET AL., "Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases", SCIENCE, (2009), vol. 325, pages 433 - 433
DESCRIPTION- GOEDDEL, GENE EXPRESSION TECHNOLOGY: METHODS IN ENZYMOLOGY, ACADEMIC PRESS, (1990), vol. 185
DESCRIPTION- GRIMM, D. ET AL., J. VIROL., (2008), vol. 82, pages 5887 - 5911
DESCRIPTION- GROENEN ET AL., MOL. MICROBIOL., (1993), vol. 10, pages 1057 - 1065
DESCRIPTION- GUSCHIN, D.Y. ET AL., "A rapid and general assay for monitoring endogenous gene modification", METHODS MOL BIOL, (2010), vol. 649, pages 247 - 256
DESCRIPTION- HASTY, P.; RIVERA-PEREZ, J.; BRADLEY, A., "The length of homology required for gene targeting in embryonic stem cells", MOL CELLBIOL, (1991), vol. 11, pages 5586 - 5591
DESCRIPTION- HERMONAT; MUZYCZKA, PNAS, (1984), vol. 81, pages 6466 - 6470
DESCRIPTION- HIGGINS DG; SHARP PM, GENE, (1988), vol. 73, no. 1, pages 237 - 244
DESCRIPTION- HOE ET AL., EMERG. INFECT. DIS., (1999), vol. 5, pages 254 - 263
DESCRIPTION- HORVATH, P.; BARRANGOU, R., "CRISPR/Cas, the immune system of bacteria and archaea", SCIENCE, (2010), vol. 327, pages 167 - 170
DESCRIPTION- HORWELL DC, TRENDS BIOTECHNOL., (1995), vol. 13, no. 4, pages 132 - 134
DESCRIPTION- HSU, P.D.; ZHANG, F., "Dissecting neural function using targeted genome engineering technologies", ACS CHEMICAL NEUROSCIENCE, (2012), vol. 3, pages 603 - 610
DESCRIPTION- HWANG, W.Y. ET AL., "Efficient genome editing in zebrafish using a CRISPR-Cas system", NAT BIOTECHNOL, (2013), vol. 31, pages 227 - 229
DESCRIPTION- ISHINO ET AL., J. BACTERIOL., (1987), vol. 169, pages 5429 - 5433
DESCRIPTION- JANSEN ET AL., MOL. MICROBIOL., (2002), vol. 43, pages 1565 - 1575
DESCRIPTION- JANSSEN ET AL., OMICS J. INTEG. BIOL., (2002), vol. 6, pages 23 - 33
DESCRIPTION- JIANG, W.; BIKARD, D.; COX, D.; ZHANG, F.; MARRAFFINI, L.A., "RNA-guided editing of bacterial genomes using CRISPR-Cas systems", NAT BIOTECHNOL, (2013), vol. 31, pages 233 - 239
DESCRIPTION- JINEK ET AL., SCIENCE, (2012), vol. 337, page 816
DESCRIPTION- JINEK, M. ET AL., "A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity", SCIENCE, (2012), vol. 337, pages 816 - 821
DESCRIPTION- JINEK, M. ET AL., "RNA-programmed genome editing in human cells", ELIFE, (2013), vol. 2, page E00471
DESCRIPTION- JOHANN ET AL., J. VIROL., (1992), vol. 66, pages 1635 - 1640
DESCRIPTION- KARGINOV; HANNON, "The CRISPR system: small RNA-guided defense in bacteria and archae", MOLE CELL, (20100115), vol. 37, no. 1, page 7
DESCRIPTION- KAUFMAN ET AL., EMBO J., (1987), vol. 6, pages 187 - 195
DESCRIPTION- KESSEL; GRUSS, SCIENCE, (1990), vol. 249, pages 374 - 379
DESCRIPTION- KOTIN, HUMAN GENE THERAPY, (1994), vol. 5, pages 793 - 801
DESCRIPTION- KREMER; PERRICAUDET, BRITISH MEDICAL BULLETIN, (1995), vol. 51, no. 1, pages 31 - 44
DESCRIPTION- KUIJAN; HERSKOWITZ, CELL, (1982), vol. 30, pages 933 - 943
DESCRIPTION- LIVINGSTONE C.D.; BARTON G.J., "Protein sequence alignments: a strategy for the hierarchical analysis of residue conservation", COMPUT. APPL. BIOSCI., (1993), vol. 9, pages 745 - 756
DESCRIPTION- LUCKLOW; SUMMERS, VIROLOGY, (1989), vol. 170, pages 31 - 39
DESCRIPTION- MAHFOUZ, M.M. ET AL., "De novo-engineered transcription activator-like effector (TALE) hybrid nuclease with novel DNA binding specificity creates double-strand breaks", PROC NATL ACAD SCI U S A, (2011), vol. 108, pages 2623 - 2628
DESCRIPTION- MAKAROVA, K.S. ET AL., "Evolution and classification of the CRISPR-Cas systems", NAT REV MICROBIOL, (2011), vol. 9, pages 467 - 477
DESCRIPTION- MALI, P. ET AL., "RNA-Guided Human Genome Engineering via Cas9", SCIENCE, (2013), vol. 339, pages 823 - 826
DESCRIPTION- MASEPOHL ET AL., BIOCHIM. BIOPHYS. ACTA, (1996), vol. 1307, pages 26 - 30
DESCRIPTION- MCLVOR EI; POLAK U; NAPIERALA M, "New insights into repeat instability: role of RNA DNA hybrids", RNA BIOL., (201009), vol. 7, no. 5, pages 551 - 558
DESCRIPTION- MICHAEL S D KORMANN ET AL., "Expression of therapeutic proteins after delivery of chemically modified mRNA in mice", NATURE BIOTECHNOLOGY, (20110109), vol. 29, pages 154 - 157
DESCRIPTION- MILLER ET AL., J. VIROL., (1991), vol. 65, pages 2220 - 2224
DESCRIPTION- MILLER, J.C. ET AL., "An improved zinc-finger nuclease architecture for highly specific genome editing", NAT BIOTECHNOL, (2007), vol. 25, pages 778 - 785
DESCRIPTION- MILLER, J.C. ET AL., "A TALE nuclease architecture for efficient genome editing", NAT BIOTECHNOL, (2011), vol. 29, pages 143 - 148
DESCRIPTION- MILLER, NATURE, (1992), vol. 357, pages 455 - 460
DESCRIPTION- MITANI; CASKEY, TIBTECH, (1993), vol. 11, pages 162 - 166
DESCRIPTION- MOJICA ET AL., MOL. MICROBIOL., (1995), vol. 17, pages 85 - 93
DESCRIPTION- MOJICA ET AL., MOL. MICROBIOL., (2000), vol. 36, pages 244 - 246
DESCRIPTION- MOL. CELL. BIOL., VOL., (1988), vol. 8, no. 1, pages 466 - 472
DESCRIPTION- MORRELL ET AL., "Crop genomics:advances and applications", NAT REV GENET., (20111229), vol. 13, no. 2, pages 85 - 96
DESCRIPTION- MOSCOU, M.J.; BOGDANOVE, A.J., "A simple cipher governs DNA recognition by TAL effectors", SCIENCE, (2009), vol. 326, page 1501
DESCRIPTION- MUSSOLINO, C. ET AL., "A novel TALE nuclease scaffold enables high genome editing activity in combination with low toxicity", NUCLEIC ACIDS RESEARCH, (2011), vol. 39, pages 9283 - 9293
DESCRIPTION- MUZYCZKA, J. CLIN. INVEST., (1994), vol. 94, page 1351
DESCRIPTION- NABEL; FELGNER, TIBTECH, (1993), vol. 11, pages 211 - 217
DESCRIPTION- NAKAMURA, Y. ET AL., "Codon usage tabulated from the international DNA sequence databases: status for the year 2000", NUCL. ACIDS RES., (2000), vol. 28, page 292
DESCRIPTION- NAKATA ET AL., J. BACTERIOL., (1989), vol. 171, pages 3553 - 3556
DESCRIPTION- National Center for Biotechnology Information, NATIONAL LIBRARY OF MEDICINE
DESCRIPTION- OLIVEIRA, T.Y. ET AL., "Translocation capture sequencing: a method for high throughput mapping of chromosomal rearrangements", J IMMUNOL METHODS, (2012), vol. 375, pages 176 - 181
DESCRIPTION- PA CARR; GM CHURCH, NATURE BIOTECHNOLOGY, (2009), vol. 27, no. 12, pages 1151 - 1162
DESCRIPTION- PEREZ, E.E. ET AL., "Establishment of HIV-1 resistance in CD4(+) T cells by genome editing using zinc-finger nucleases", NAT BIOTECHNOL, (2008), vol. 26, pages 808 - 816
DESCRIPTION- PINKERT ET AL., GENES DEV., (1987), vol. 1, pages 268 - 277
DESCRIPTION- PLOSKER GL ET AL., "Fluvastatin: a review of its pharmacology and use in the management of hypercholesterolaemia", DRUGS, (1996), vol. 51, no. 3, pages 433 - 459
DESCRIPTION- PORTEUS, M.H.; BALTIMORE, D., "Chimeric nucleases stimulate gene targeting in human cells", SCIENCE, (2003), vol. 300, page 763
DESCRIPTION- PROC. NATL. ACAD. SCI. USA., (1981), vol. 78, no. 3, pages 1527 - 1531
DESCRIPTION- QI, L.S. ET AL., "Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression", CELL, (2013), vol. 152, pages 1173 - 1183
DESCRIPTION- QUEEN; BALTIMORE, CELL, (1983), vol. 33, pages 741 - 748
DESCRIPTION- RAN ET AL., CELL, (20130912), vol. 154, no. 6, pages 1380 - 1389
DESCRIPTION- REMY ET AL., BIOCONJUGATE CHEM., (1994), vol. 5, pages 647 - 654
DESCRIPTION- REYON, D. ET AL., "FLASH assembly of TALENs for high-throughput genome editing", NAT BIOTECHNOL, (2012), vol. 30, pages 460 - 465
DESCRIPTION- SALEH-GOHARI, N.; HELLEDAY, T., "Conservative homologous recombination preferentially repairs DNA double-strand breaks in the S phase of the cell cycle in human cells", NUCLEIC ACIDS RES, (2004), vol. 32, pages 3683 - 3688
DESCRIPTION- SAMULSKI ET AL., J. VIROL., (1989), vol. 63, pages 03822 - 3828
DESCRIPTION- SANDER, J.D. ET AL., "Selection-free zinc-finger-nuclease engineering by context-dependent assembly (CoDA", NAT METHODS, (2011), vol. 8, pages 67 - 69
DESCRIPTION- SANJANA, N.E. ET AL., "A transcription activator-like effector toolbox for genome engineering", NATURE PROTOCOLS, (2012), vol. 7, pages 171 - 192
DESCRIPTION- SAPRANAUSKAS ET AL., NUCLEIC ACIS RESEARCH, (2011), vol. 39, page 9275
DESCRIPTION- SAPRANAUSKAS, R. ET AL., "The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli", NUCLEIC ACIDS RES, (2011), vol. 39, pages 9275 - 9282
DESCRIPTION- SCHROEDER, A.; LEVINS, C.; CORTEZ, C.; LANGER, R.; ANDERSON, D., "Lipid-based nanotherapeutics for siRNA delivery", JOURNAL OF INTERNAL MEDICINE, (2010), vol. 267, pages 9 - 21
DESCRIPTION- SCHULTZ ET AL., GENE, (1987), vol. 54, pages 113 - 123
DESCRIPTION- SEED, NATURE, (1987), vol. 329, page 840
DESCRIPTION- series METHODS IN ENZYMOLOGY, ACADEMIC PRESS, INC.
DESCRIPTION- SHALEK ET AL., "Nanowire-mediated delivery enables functional interrogation of primary immune cells: application to the analysis of chronic lymphocytic leukemia", NANO LETTERS, (20121212), vol. 12, no. 12, pages 6498 - 6504
DESCRIPTION- SHEN, B. ET AL., "Generation of gene-modified mice via Cas9/RNA-mediated gene targeting", CELL RES, (2013), vol. 23, pages 720 - 723
DESCRIPTION- SIMON RJ ET AL., PNAS, (1992), vol. 89, no. 20, pages 9367 - 9371
DESCRIPTION- SMITH ET AL., MOL. CELL. BIOL., (1983), vol. 3, pages 2156 - 2165
DESCRIPTION- SMITHIES, O.; GREGG, R.G.; BOGGS, S.S.; KORALEWSKI, M.A.; KUCHERLAPATI, R.S., "Insertion of DNA sequences into the human chromosomal beta-globin locus by homologous recombination", NATURE, (1985), vol. 317, pages 230 - 234
DESCRIPTION- SMITH; JOHNSON, GENE, (1988), vol. 67, pages 31 - 40
DESCRIPTION- SOLDNER, F. ET AL., "Generation of isogenic pluripotent stem cells differing exclusively at two early onset Parkinson point mutations", CELL, (2011), vol. 146, pages 318 - 331
DESCRIPTION- SOMMNERFELT ET AL., VIROL., (1990), vol. 176, pages 58 - 59
DESCRIPTION- STUDIER ET AL., GENE EXPRESSION TECHNOLOGY: METHODS IN ENZYMOLOGY, ACADEMIC PRESS, (1990), vol. 185, pages 60 - 89
DESCRIPTION- SU X; FRICKE J; KAVANAGH DG; IRVINE DJ, "In vitro and in vivo mRNA delivery using lipid-enveloped pH-responsive polymer nanoparticles", MOL PHARM., (20110401), vol. 8, no. 3, pages 774 - 787
DESCRIPTION- TAKASU, Y. ET AL., "Targeted mutagenesis in the silkworm Bombyx mori using zinc finger nuclease mRNA injection", INSECT BIOCHEM MOLEC, (2010), vol. 40, pages 759 - 765
DESCRIPTION- TANG J ET AL., "Inhibition of SREBP by a Small Molecule, Betulin, Improves Hyperlipidemia and Insulin Resistance and Reduces Atherosclerotic Plaques", CELL METABOLISM, (20110105), vol. 13, no. 1, pages 44 - 56
DESCRIPTION- TAYLOR W.R., "The classification of amino acid conservation", J. THEOR. BIOL., (1986), vol. 119, pages 205 - 218
DESCRIPTION- THOMAS, K.R.; FOLGER, K.R.; CAPECCHI, M.R., "High frequency targeting of genes to specific sites in the mammalian genome", CELL, (1986), vol. 44, pages 419 - 428
DESCRIPTION- TRAPANI ET AL., "Potential role of nonstatin cholesterol lowering agents", IUBMB LIFE, (201111), vol. 63, no. 11, pages 964 - 971
DESCRIPTION- TRATSCHIN ET AL., MOL. CELL. BIOL., (1984), vol. 4, pages 2072 - 2081
DESCRIPTION- TRATSCHIN ET AL., MOL. CELL. BIOL., (1985), vol. 5, pages 3251 - 3260
DESCRIPTION- TREMBLAY ET AL., "Transcription Activator-Like Effector Proteins Induce the Expression of the Frataxin Gene", HUMAN GENE THERAPY, (201208), vol. 23, no. 8, pages 883 - 890
DESCRIPTION- TUSCHL, T., "Expanding small RNA interference", NAT BIOTECHNOL, (2002), vol. 20, pages 446 - 448
DESCRIPTION- URNOV, F.D.; REBAR, E.J.; HOLMES, M.C.; ZHANG, H.S.; GREGORY, P.D., "Genome editing with engineered zinc finger nucleases", NAT REV GENET, (2010), vol. 11, pages 636 - 646
DESCRIPTION- VALTON, J. ET AL., "Overcoming transcription activator-like effector (TALE) DNA binding domain sensitivity to cytosine methylation", J BIOL CHEM, (2012), vol. 287, pages 38427 - 38432
DESCRIPTION- VAN BRUNT, BIOTECHNOLOGY, (1988), vol. 6, no. 10, pages 1149 - 1154
DESCRIPTION- VAN EMBDEN ET AL., J. BACTERIOL., (2000), vol. 182, pages 2393 - 2401
DESCRIPTION- VIGNE, RESTORATIVE NEUROLOGY AND NEUROSCIENCE, (1995), vol. 8, pages 35 - 36
DESCRIPTION- WANG, H. ET AL., "One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering", CELL, (2013), vol. 153, pages 910 - 918
DESCRIPTION- WATANABE, T. ET AL., "Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases", NAT COMMUN, (2012), page 3
DESCRIPTION- WEST ET AL., VIROLOGY, (1987), vol. 160, pages 38 - 47
DESCRIPTION- WILSON, E.B., "Probable inference, the law of succession, and statistical inference", J AM STAT ASSOC, (1927), vol. 22, pages 209 - 212
DESCRIPTION- WILSON ET AL., J. VIROL., (1989), vol. 63, pages 2374 - 2378
DESCRIPTION- WINOTO; BALTIMORE, EMBO J., (1989), vol. 8, pages 729 - 733
DESCRIPTION- WOOD, A.J. ET AL., "Targeted genome editing across species using ZFNs and TALENs", SCIENCE, (2011), vol. 333, page 307
DESCRIPTION- WU, S.; YING, G.X.; WU, Q.; CAPECCHI, M.R., "A protocol for constructing gene targeting vectors: generating knockout mice for the cadherin family and beyond", NAT PROTOC, (2008), vol. 3, pages 1056 - 1076
DESCRIPTION- XIA CF; BOADO RJ; PARDRIDGE WM, "Antibody-mediated targeting of siRNA via the human insulin receptor using avidin-biotin technology", MOL PHARM., (200905), vol. 6, no. 3, pages 747 - 751
DESCRIPTION- YU ET AL., GENE THERAPY, (1994), vol. 1, pages 13 - 26
DESCRIPTION- ZHANG Y; SCHLACHETZKI F; PARDRIDGE WM., "Global non-viral gene transfer to the primate brain following intravenous administration", MOL THER., (200301), vol. 7, no. 1, pages 11 - 18
DESCRIPTION- ZUKER; STIEGLER, NUCLEIC ACIDS RES., (1981), vol. 9, pages 133 - 148
OPPOSITION- AKOPIAN et al., PNAS, (20030000), vol. 100, pages 8688 - 8691
OPPOSITION- Anonymous, "For Investigators: Participation Agreement", Harvard Medical School, (20060505), URL: https://hms.harvard.edu/, XP055751759
OPPOSITION- anonymous, "Preparing a proposal", Harvard, (20110928), XP055751732
OPPOSITION- BARRANGOU, 2012 : Nat Biotechnol ., (20120000), vol. 30, no. 9, pages 836 - 8
OPPOSITION- BEERLI et al., PNAS, (19980000), vol. 95, pages 14628 - 14633
OPPOSITION- BEERLI et al., PNAS, (20000000), vol. 97, pages 1495 - 1500
OPPOSITION- BIANCAFORT et al., Molecular Pharmacology, (20040000), vol. 66, pages 1361 - 1371
OPPOSITION- BODEN, Mol Ther, (20040000), vol. 9, pages 396 - 402
OPPOSITION- BOLOTIN, Microbiology, (20050000), vol. 151, page 2551
OPPOSITION- BROUNS. S., Science, (20120000), vol. 337, pages 808 - 809
OPPOSITION- BRUNET, PNAS, (20090000), vol. 106, pages 10620 - 10625
OPPOSITION- BULTMANN, Nucleic Acids Research, (20120000), vol. 40, pages 5368 - 77
OPPOSITION- CAMPEAU, E. et al., PLoS One, (20090000), vol. 4, page e6529
OPPOSITION- CARLSON, PNAS, (20120000), vol. 109, pages 17382 - 17387
OPPOSITION- CARROLL et al., Gene Ther, (20080000), vol. 15, pages 1463 - 1468
OPPOSITION- CERMAK, Nucleic Acids Res, (20110000), vol. 39, pages 1 - 11
OPPOSITION- CHANG et al., Cell Research, (20130000), vol. 23, no. 4, pages 465 - 472
OPPOSITION- Chenfei Zhang ; Aijian Qin ; Hongjun Chen ; Xufang Deng ; Yuwen Su ; Kun Qian, "MDV-1 VP22: a transporter that can selectively deliver proteins into cells", Archives of Virology ; Official Journal of the Virology Divisionof the International Union of Microbiological Societies, Springer-Verlag, Vi, Vi , (20090604), vol. 154, no. 7, ISSN 1432-8798, pages 1027 - 1034, XP019722961
OPPOSITION- CHO, S. et al., Nature Biotechnology, (20130000), vol. 31, pages 230 - 232
OPPOSITION- CHOULIKA, J Virol, (19960000), vol. 70, pages 1792 - 1798
OPPOSITION- CHRISTIAN et al., Genetics, (20100000), vol. 186, pages 757 - 761
OPPOSITION- CONG, L. et al., Science, (20130000), vol. 339, no. 6121, pages 819 - 823
OPPOSITION- D220 - Vossius Grounds of Appeal filed in EP-B1 2898075 case
OPPOSITION- D221 - Vossius submission filed on August 2, 2022 in EP-B1 2898075 case
OPPOSITION- D222 - Vossius submission filed on October 9, 2023 in EP-B1 2898075 case
OPPOSITION- D223 - Concordance list for the documents cited in the relevant parts of our D220, D221, and D222 vs. documents of present case
OPPOSITION- D230 - Sequence alignment of D40 vs. D100 (D11 and D120 of EP-B1 2898075 case)
OPPOSITION- D231 - Sequence match between tracrRNA sequence annotated in D98 (D119a in EP-B1 2898075) vs. Figs. 12 & 13W of present and '075 patent
OPPOSITION- DAI et al., J. Biol. Chem., (20020000), vol. 277, no. 27, pages 24390 - 8
OPPOSITION- DICARLO et al., Nucleic Acids Res, (20130000), vol. 41, pages 4336 - 4343
OPPOSITION- DINGWALL, CELL, (19820000), vol. 30, pages 449 - 58
OPPOSITION- DINGWALL, Cell Biol., (19880000), vol. 107, pages 841 - 849
OPPOSITION- DO et al., FEBS Letters, (20060000), vol. 580, no. 7, pages 1865 - 1871
OPPOSITION- ESPINOZA, RNA, (20070000), vol. 13, pages 583 - 596
OPPOSITION- ESVELT, Nature Methods, (20130000), vol. 10, pages 1116 - 1121
OPPOSITION- FIECK et al., Nucleic Acids Res, (19920000), vol. 20, no. 7, pages 1785 - 1791
OPPOSITION- FISCHER-FANTUZZI et al., Mol Cell Bio l, (19880000), vol. 8, no. 12, pages 5495 - 5503
OPPOSITION- FOECKING, Gene, (19860000), pages 101 - 5
OPPOSITION- FREITAS, Curr. Genom, (20090000), vol. 10, pages 550 - 557
OPPOSITION- FRIEDLAND, Genome Biology, (20150000), vol. 16, page 257
OPPOSITION- GEISSLER et al., PloS One, (20110000), vol. 6, page e19509
OPPOSITION- GenBank, Database accession no. CCK74173.1
OPPOSITION- Genbank, Database accession no. HE980450
OPPOSITION- GILBERT, Cell, (20130000), vol. 154, pages 442 - 451
OPPOSITION- GORDLEY, PNAS, (20090000), vol. 106, pages 5053 - 5058
OPPOSITION- GRATZ, Genetics, (20130000), vol. 194, pages 1029 - 35
OPPOSITION- HANDEL, E. et al., Human Gene Ther, (20120000), vol. 23, pages 321 - 329
OPPOSITION- HAN, Molecules and Cells, (20000000), vol. 10, pages 728 - 732
OPPOSITION- HORVATH, J. Bacteriol, (20080000), vol. 190, page 1401
OPPOSITION- HSU, Nature Biotechnology, (20130000), vol. 31, pages 827 - 832
OPPOSITION- HWANG, W. et al., Nature Biotechnology, (20130000), vol. 31, pages 227 - 229
OPPOSITION- IMAGAWA, FEBS Lett, (20000000), vol. 484, pages 118 - 124
OPPOSITION- JENSEN et al., Journal of Biomedical Science, (20110000), vol. 18, page 10
OPPOSITION- JINEK et al., Science, (20130000), vol. 337, pages 816 - 821
OPPOSITION- JINEK. M. et al., Science, (20120000), vol. 337, pages 816 - 821
OPPOSITION- KALDERON, Cell, (19840000), vol. 39, pages 499 - 509
OPPOSITION- KINNEVEY et al., Antimicrob Agents Chemother, (20130000), vol. 57, no. 1, pages 524 - 531
OPPOSITION- LANGE, J Biol Chem, (20070000), vol. 282, pages 5101 - 5105
OPPOSITION- LEE, Genome Res, (20100000), vol. 20, pages 81 - 89
OPPOSITION- LEE, Genome Res, (20120000), vol. 22, pages 539 - 548
OPPOSITION- LEI, Molecular Therapy, (20110000), vol. 19, no. 5, pages 942 - 950
OPPOSITION- LEMAY, Chemistry & Biology, (20060000), vol. 13, pages 857 - 868
OPPOSITION- LIU, Biotechnol Bioeng, (20100000), vol. 106, pages 97 - 105
OPPOSITION- LOMBARDO, Nature Biotechnology, (20070000), vol. 11, pages 1298 - 1306
OPPOSITION- LUO et al., Traffic, (20040000), vol. 5, no. 11, pages 847 - 854
OPPOSITION- MAHFOUZ et al., Plant Molecular Biology, (20120000), vol. 78, pages 311 - 321
OPPOSITION- MAKAROVA, Biology Direct, (20110000), vol. 6, page 38
OPPOSITION- MALI, P. et al., Science, (20130000), vol. 339, pages 823 - 826
OPPOSITION- MA, PLoS One, (20120000), vol. 7, page e45035
OPPOSITION- MERCER, Nucleic Acids Research, (20120000), vol. 40, pages 11163 - 72
OPPOSITION- MILLER, Nature Biotechnology, (20110000), vol. 29, pages 143 - 148
OPPOSITION- MOJICA et al., Journal of Molecular Evolution, (20050000), vol. 60, page 174
OPPOSITION- New England Biolabs
OPPOSITION- NOMURA et al., Journal of the American Chemical Society, (20070000), vol. 129, pages 8676 - 8677
OPPOSITION- NOZAWA, PLoS One, (20110000), vol. 6, no. 5, page e19543
OPPOSITION- Nucleotide, (20130103), Database accession no. HE980450, URL: NCBI, XP055752238
OPPOSITION- PAPWORTH et al., PNAS, (20030000), vol. 100, pages 1621 - 1626
OPPOSITION- PARK et al., J. Biol. Chem., (20020000), vol. 277, no. 35, pages 31423 - 31429
OPPOSITION- PEREZ-PINERA, Curr Opin Chem Biol, (20120000), vol. 16, pages 268 - 277
OPPOSITION- PLANEV. et al., J. Biol. Chem., (20020000), vol. 277, no. 44, pages 42188 - 96
OPPOSITION- REBAR, Nature Medicine, (20020000), vol. 8, pages 1427 - 32
OPPOSITION- RIVENBARK, Epigenetics, (20120000), vol. 7, pages 350 - 360
OPPOSITION- ROBERTS, Cell, (19870000), vol. 50, pages 465 - 475
OPPOSITION- RUBEN S, ET AL., "STRUCTURAL AND FUNCTIONAL CHARACTERIZATION OF HUMAN IMMUNODEFICIENCY VIRUS TAT PROTEIN", Journal of Virology, THE AMERICAN SOCIETY FOR MICROBIOLOGY, US, US , (19890101), vol. 63, no. 01, ISSN 0022-538X, pages 01 - 08, XP009013914
OPPOSITION- SANJANA, Nature Protocol, (20130000), vol. 7, pages 171 - 192
OPPOSITION- SATO, Biosci Biotechnol Biochem, (20090000), vol. 73, pages 2145 - 2148
OPPOSITION- SCHRAMM, Genes Dev, (20020000), vol. 16, pages 2593 - 2620
OPPOSITION- SHAH, Biochem. Soc. Tran, (20090000), vol. 37, pages 23 - 28
OPPOSITION- SHIEH, ET AL. et al., Plant Physiol, (19930000), vol. 101, pages 353 - 361
OPPOSITION- SNOWDEN, Current Biology, (20020000), vol. 12, pages 2159 - 2166
OPPOSITION- SOREK, Nature Review Microbiology, (20080000), vol. 6, page 181
OPPOSITION- "Staphylococcus aureus subsp. aureus ORFX gene and pseudo SCCmec-SCC-SCCCRISPR element., strain M06/0171", Nucleotide, (20130103), Database accession no. HE980450, URL: ncbi, XP055338801
OPPOSITION- TAN et al., Journal of Virology, (20060000), vol. 80, pages 1939 - 1948
OPPOSITION- TAN et al., PNAS, (20030000), vol. 100, pages 11997 - 12002
OPPOSITION- TINLAND et al., Proc. Natl. Acad. Sei., (19920000), vol. 89, pages 7442 - 7446
OPPOSITION- TISCORNIA et al., Delivery and expression of DNA and RNA
OPPOSITION- "transcription", ALBERTS B. et al., Molecular Biology of the Cell, Garland Science, (20020000), page G-35, XP055751721
OPPOSITION- "transcription", LODISH H. et al., Molecular Cell Biology. 6th ed., W. H. Freeman and Company, (20080000), page G-22, XP055751718
OPPOSITION- TRUANT R, CULLEN B R, "The arginine-rich domains present in human immunodeficiency virus type 1 tat and rev function as direct importin B-dependent nuclear localization signals", MOLECULAR AND CELLULAR BIOLOGY, American Society for Pharmacology and Experimental Therapeutics, US, US , (19990201), vol. 19, no. 2, ISSN 0270-7306, pages 1210 - 1217, XP002964815
OPPOSITION- URL: http://www.addgene.org/vector- database/4288
OPPOSITION- URNOV, Nature, (20050000), vol. 435, pages 646 - 651
OPPOSITION- VAN DEN ACKERVEKEN, Cell, (19960000), vol. 8, pages 1307 - 1316
OPPOSITION- VILLION et al., Cell R esearch, (20130000), vol. 23, pages 15 - 17
OPPOSITION- WALSH et al., "A variant CRISPR-Cas9 system adds versatility to genome engineering", PNAS, (20130000), vol. 110, no. 39, pages 15514 - 15515, XP055338779
OPPOSITION- WANG et al., Cell, (20130000), vol. 153, no. 4, pages 910 - 918
OPPOSITION- WOLFF, Nature Biotechnology, (20010000), vol. 19, pages 1118 - 1120
OPPOSITION- WU, The American Society of Gene & Cell Therapy, (20100000), vol. 18, pages 80 - 86
OPPOSITION- XU, Fruit Quarterly, New York, (20130000), vol. 21, pages 19 - 22
OPPOSITION- YU, Genetics, (20130000), vol. 195, pages 289 - 291
OPPOSITION- ZHANG et al., Nature Biotechnology, (20110000), vol. 29, no. 2, pages 149 - 53
OPPOSITION- Elena Senis et al., "CRISPR/Cas9-mediated genome engineering: An adeno-associated viral (AAV) vector toolbox", Biotechnol J, (2014), vol. 9, doi:10.1002/biot.201400046, XP002737562
OPPOSITION- Archana Chugh, François Eudes, Youn-Seb Shim, "Cell-penetrating peptides: Nanocarrier for macromolecule delivery in living cells", IUBMB Life, Published for the International Union of Biochemistry and Molecular Biology by Taylor & Francis, (20100325), vol. 62, no. 3, doi:10.1002/iub.297, ISSN 15216543, pages 183 - 193, XP055071760
OPPOSITION- MI Z, ET AL., "CHARACTERIZATION OF A CLASS OF CATIONIC PEPTIDES ABLE TO FACILITATEEFFICIENT PROTEIN TRANSDUCTION IN VITRO AND IN VIVO", Molecular Therapy, Elsevier Inc., US, US , (20001001), vol. 02, no. 04, doi:10.1006/mthe.2000.0137, ISSN 1525-0016, pages 339 - 347, XP000998662
OPPOSITION- PATTERSON STACEY S ET AL, "Codon optimization of bacterial luciferase (lux) for expression in mammalian cells", Journal of Industrial Microbiology & Biotechnology, BASINGSTOKE, GB, GB , (20050301), vol. 32, no. 3, doi:10.1007/s10295-005-0211-8, ISSN 1367-5435, pages 115 - 123, XP002503994
OPPOSITION- WU et al., "Target specificity of the CRISPR-Cas 9 system", Quant Biol., (20140600), vol. 2, no. 2, pages 59 - 70, XP055176829
OPPOSITION- SMITH et al., "Identification of common molecular subsequences", Journal of Molecular Biology, (19810325), vol. 147, no. 1, doi:10.1016/0022-2836(81)90087-5, pages 195 - 197, XP024015032
OPPOSITION- Chung-Pin Chen; Jyh-Ching Chou; Betty Revon Liu; Microsugar Chang; Han-Jung Lee, "Transfection and expression of plasmid DNA in plant cells by an arginine‐rich intracellular delivery peptide without protoplast preparation", FEBS Letters, ELSEVIER, AMSTERDAM., NL, NL , (20070409), vol. 581, no. 9, doi:10.1016/j.febslet.2007.03.076, ISSN 0014-5793, pages 1891 - 1897, XP071249466
OPPOSITION- Yan Zhang et al., "Processing-Independent CRISPR RNAs Limit Natural Transformation in Neisseria meningitidis", Molecular Cell, (20130523), vol. 50, doi:10.1016/j.molcel.2013.05.001, XP028553287
OPPOSITION- Duchêne Benjamin L., Cherif Khadija, Iyombe-Engembe Jean-Paul, Guyon Antoine, Rousseau Joel, Ouellet Dominique L., Barbeau Xavier, Lague Patrick, Tremblay Jacques P., "CRISPR-Induced Deletion with SaCas9 Restores Dystrophin Expression in Dystrophic Models In Vitro and In Vivo", Molecular Therapy, Elsevier Inc, United States, United States, (20181107), vol. 26, no. 11, doi:10.1016/j.ymthe.2018.08.010, ISSN 1525-0016, pages 2604 - 2616, XP055868240
OPPOSITION- YANG et al., "HIV TAT-mediated protein transduction and subcellular localization using novel expression vectors", FEBS Letters, (20020000), vol. 532, doi:10.1016/S0014-5793(02)03624-4, pages 36 - 44, XP004395339
OPPOSITION- Yang, Y. Ma, J. Song, Z. Wu, M., "HIV-1 TAT-mediated protein transduction and subcellular localization using novel expression vectors", FEBS Letters, ELSEVIER, AMSTERDAM., NL, NL , (20021204), vol. 532, no. 1-2, doi:10.1016/S0014-5793(02)03624-4, ISSN 0014-5793, pages 36 - 44, XP004395339
OPPOSITION- SNOWDEN et al., "Gene -specific targeting of H3K9 methylation is sufficient for initiating repression in vivo", Current Biology, (20020000), vol. 12, doi:10.1016/S0960-9822(02)01391-X, pages 2159 - 2166, XP009157323
OPPOSITION- ELLIS et al., "Zinc-finger nuclease-mediated gene correction using single AAV vector transduction and enhancement by Food and Drug Administration-approved drugs", Gene Ther., (20120119), vol. 20, no. 1, doi:10.1038/gt.2011.211, pages 35 - 42, XP002730955
OPPOSITION- WU et al., "Effect of Genome Size on AAV Vector Packaging", Molecular Therapy, (20091110), vol. 18, no. 1, doi:10.1038/mt.2009.255, pages 80 - 86, XP055235504
OPPOSITION- LAI et al., "Evidence for the Failure of Adeno- associated Virus Serotype 5 to Package a Viral Genome >= 8.2 kb", Molecular Therapy, (20091110), vol. 18, no. 1, doi:10.1038/mt.2009.256, pages 75 - 79, XP055217887
OPPOSITION- DONG et al., "Characterization of Genome Integrity for Oversized Recombinant AAV Vector", Molecular Therapy, (20091110), vol. 18, no. 1, doi:10.1038/mt.2009.258, pages 87 - 92, XP055183270
OPPOSITION- HIRSCH et al., "Little Vector, Big Gene Transduction: Fragmented Genome Reassembly of Adeno-associated Virus", Molecular Therapy, (20100101), vol. 18, no. 1, doi:10.1038/mt.2009.280, pages 6 - 8, XP055312618
OPPOSITION- Elitza Deltcheva, Krzysztof Chylinski, Cynthia M. Sharma, Karine Gonzales, Yanjie Chao, Zaid A. Pirzada, Maria R. Eckert, Jörg Vogel, Emmanuelle Charpentier, "CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III", Nature, (20110331), vol. 471, no. 7340, doi:10.1038/nature09886, pages 602 - 607, XP055308803
OPPOSITION- RAN et al., "In vivo genome editing using Staphylococcus aureus Cas9", Nature, (20150401), vol. 520, doi:10.1038/nature14299, pages 186 - 191, XP055484527
OPPOSITION- Benjamin P Kleinstiver et al., "Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition", Nature Biotechnology, (2015), vol. 33, doi:10.1038/nbt.3404, XP055309933
OPPOSITION- ESVELT et al., "Orthogonal Cas9 proteins for RNA-guided gene regulation and editing", Nature Methods, (20130000), vol. 11, doi:10.1038/nmeth.2681, pages 1116 - 1121, XP055128928
OPPOSITION- Fyodor D. Urnov, Edward J. Rebar, Michael C. Holmes, H. Steve Zhang, Philip D. Gregory, "Genome editing with engineered zinc finger nucleases", Nature Reviews Genetics, Nature Pub. Group, (20100901), vol. 11, no. 9, doi:10.1038/nrg2842, ISSN 14710056, pages 636 - 646, XP055198280
OPPOSITION- J. Keith Joung, Sander Jeffry D., "TALENs: a widely applicable technology for targeted genome editing", Nature Reviews Molecular Cell Biology, Nature Publishing Group UK, London, London, vol. 14, no. 1, doi:10.1038/nrm3486, ISSN 1471-0072, pages 49 - 55, XP055282847
OPPOSITION- Makarova Kira S; Haft Daniel H; Barrangou Rodolphe; Brouns Stan J J; Charpentier Emmanuelle; Horvath Philippe; Moineau Sylvain; Mojica Francisco J M; Wolf Yuri I; Yakunin Alexander F; van der Oost John; Koonin Eugene V, "Evolution and classification of the CRISPR-Cas systems", Nature Reviews Microbiology, NATURE PUBLISHING GROUP, GB, GB , (20110509), vol. 9, no. 6, doi:10.1038/nrmicro2577, ISSN 1740-1526, pages 467 - 477, XP009155547
OPPOSITION- Morgan L. Maeder et al., "Development of a gene-editing approach to restore vision loss in Leber congenital amaurosis type 10", Nature Medicine, (2019), vol. 25, doi:10.1038/s41591-018-0327-9, XP036693196
OPPOSITION- GORDLEY et al., "Synthesis of programmable integrases", PNAS, (20090000), vol. 106, no. 13, doi:10.1073/pnas.0812502106, pages 5053 - 5058, XP002544501
OPPOSITION- MAHOWALD et al., "Characterizing a model human gut microbiota composed of members of its two dominant bacterial phyla", PNAS, (20090407), vol. 106, no. 14, doi:10.1073/pnas.0901529106, pages 5859 - 5864, XP055087929
OPPOSITION- GASIUNAS et al., "Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria.", Proceedings of the National Academy of Sciences, (20120000), vol. 109, no. 39, doi:10.1073/pnas.1208507109, pages E2579 - E2586, XP055569955
OPPOSITION- HOU et al., "Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis", PNAS, (20130000), vol. 110, no. 39, doi:10.1073/pnas.1313587110, pages 15644 - 15649, XP055118866
OPPOSITION- Z. Hou, Y. Zhang, N. E. Propson, S. E. Howden, L.-F. Chu, E. J. Sontheimer, J. A. Thomson, "Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis", Proceedings of the National Academy of Sciences, National Academy of Sciences, (20130924), vol. 110, no. 39, doi:10.1073/pnas.1313587110, ISSN 00278424, pages 15644 - 15649, XP055118866
OPPOSITION- BEERLI et al., "Toward controlling gene expression at will: Specific regulation of D18 the erbB-2 / HER-2 promoter by using polydactyl zinc finger proteins constructed from modular building blocks", PNAS, (19980000), vol. 95, doi:10.1073/pnas.95.25.14628, pages 14628 - 14633, XP002924795
OPPOSITION- Daniele Derossi, Sophie Calvet, Alain Trembleau, Alié Brunissen, Gérard Chassaing and Alain Prochiantz, "Cell Internalization of the Third Helix of the Antennapedia Homeodomain Is Receptor-independent", Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, US, US , (19960726), vol. 271., no. 30., doi:10.1074/jbc.271.30.18188, ISSN 0021-9258, pages 18188 - 18193, XP002024485
OPPOSITION- DEROSSI et al., "Cell internalization of the third helix of the Antennapedia homeodomain is receptor-independent", J. Biol. Chem., (19960000), vol. 271, doi:10.1074/jbc.271.30.18188, pages 18188 - 18193, XP002024485
OPPOSITION- EFTHYMIADIS A, BRIGGS L J, JANS D A, "The HIV-1 tat nuclear localization sequence confers novel nuclear import properties", Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, US, US , (19980116), vol. 273, no. 3, doi:10.1074/jbc.273.3.1623, ISSN 0021-9258, pages 1623 - 1628, XP003000854
OPPOSITION- HÄNDEL et al., "Versatile and efficient genome editing in human cells by D9 combining zinc-finger nucleases with adeno-associated viral vectors", Human Gene Ther, (20111214), vol. 23, no. 3, pages 321 - 329, XP002730956
OPPOSITION- GRISSA et al., "CRISPRFinder: a web tool to identify clustered regularly interspaced short palindromic repeats", Nucleic Acids Res., (20070700), vol. 35, doi:10.1093/nar/gkm360, pages W52 - W57, XP002490217
OPPOSITION- Rimantas Sapranauskas et al., "The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli", Nucleic Acids Research, (2011), vol. 39, doi:10.1093/nar/gkr606, XP093259587
OPPOSITION- MERCER A. C. et al., "Chimeric TALE recombinases with programmable DNA sequence specificity", Nucleic Acids Research, (20120000), vol. 40, no. 21, doi:10.1093/nar/gks875, pages 11163 - 11172, XP055133292
OPPOSITION- DICARLO et al., "Genome engineering in Saccharomyces cerevisiae using CRISPR- Cas systems", Nucleic Acids Research, (20130000), vol. 41, no. 7, doi:10.1093/nar/gkt135, pages 4336 - 4343, XP055086617
OPPOSITION- AGUDELO et al., "Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9", Genome Res, (20200103), vol. 30, pages 107 - 117, XP055752359
OPPOSITION- BLANCAFORT et al., "Designing transcription factor architectures for drug discovery", Molecular Pharmacology, (20040000), vol. 66, no. 6, doi:10.1124/mol.104.002758, pages 1361 - 1371, XP055015641
OPPOSITION- JINEK et al., "A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.", Science, (20120000), vol. 337, doi:10.1126/science.1225829, pages 816 - 821, XP055549487
OPPOSITION- JINEK et al., "A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity", Science, (20120817), vol. 337, doi:10.1126/science.1225829, pages 816 - 821, XP055549487
OPPOSITION- BROUNS, "A Swiss Army Knife of Immunity", Science, (20120817), vol. 337, doi:10.1126/science.1227253, pages 808 - 809, XP055113718
OPPOSITION- CONG et al., "Multiplex genome engineering using CRISPR/Cas systems", Science, (20130000), vol. 339, doi:10.1126/science.1231143, pages 819 - 823, XP055458249
OPPOSITION- CONG et al., "Multiplex Genome Engineering Using CRISPR/Cas Systems", Science, (20130103), vol. 339, doi:10.1126/science.1231143, pages 819 - 823, XP055300065
OPPOSITION- MALI et al., "RNA-guided human genome engineering via Cas9", Science, (20130000), vol. 339, doi:10.1126/science.1232033, pages 823 - 826, XP055469277
OPPOSITION- GUO et al., "Group II introns designed to insert into therapeutically relevant DNA target sites in human cells", Science, (20000000), vol. 289, doi:10.1126/science.289.5478.452, pages 452 - 457, XP002938491
OPPOSITION- KINNEVEY et al., "Emergence of Sequence Type 779 Methicillin-Resistant Staphylococcus aureus Harboring a Novel Pseudo Staphylococcal Cassette Chromosome mec (SCCmec)-SCC- SCCCRISPR Composite Element in Irish Hospitals", Antimicrobial Agents and Chemotherapy, (20130100), vol. 57, no. 1, doi:10.1128/AAC.01689-12, pages 524 - 531, XP055338796
OPPOSITION- KINNEVEY et al., "Emergence of sequence type 779 methycillin-resistant D15 Staphylococcus aureus harboring a novel pseudo staphylococcal cassette chromosome mec (SCCmec)-SCC-SCCCRISPR composite element in Irish hospitals", Antimicrobial Agents and Chemotherapy, (20130000), vol. 57, no. 1, doi:10.1128/AAC.01689-12, pages 524 - 531, XP055338796
OPPOSITION- JENSEN et al., "An update on targeted gene repair in mammalian cells: methods and mechanisms", J. Biomed. Sci., (20110202), vol. 18, no. 1, pages 1 - 10, XP021085735
OPPOSITION- CAMACHO et al., "BLAST+: architecture and applications", BMC Bioinformatics, (20091215), vol. 10, doi:10.1186/1471-2105-10-421, page 421, XP055111342
OPPOSITION- FRIEDLAND et al., "Characterization of Staphylococcus aureus Cas9: a smaller cas9 D8 for all-in-one adeno-associated virus delivery and paired nickase applications", Genome Biology, (20151124), vol. 16, no. 1, page 257, XP055347837
OPPOSITION- HAFT et al., "A Guild of 45 CRISPR-Associated (Cas) Protein Families and Multiple CRISPR/Cas Subtypes Exist in Prokaryotic Genomes", PLoS Comput Biol, (20051111), vol. 1, no. 6, page e60, XP002574577
OPPOSITION- Christopher S. Raymond, Philippe Soriano, "High-Efficiency FLP and ΦC31 Site-Specific Recombination in Mammalian Cells", PLOS ONE, Public Library of Science, US, US , (20070101), vol. 2, no. 1, doi:10.1371/journal.pone.0000162, ISSN 1932-6203, pages e162/1 - 4, XP055069825
OPPOSITION- MASTROIANNI et al., "Group II intron-based gene targeting reactions in eukaryotes", PLoS ONE, (20080901), vol. 3, no. 9, page e3121, XP055149290
OPPOSITION- NOGUCHI et al., "PDX-1 containing its own Antennapedia-like protein transduction domain can transduce pancreatic duct and islet cells", Diabetes, (20030000), vol. 52, doi:10.2337/diabetes.52.7.1732, pages 1732 - 1737, XP002549995
OPPOSITION- Noguchi H ET AL, "PDX-1 Protein Containing Its Own Antennapedia-Like Protein Transduction Domain Can Transduce Pancreatic Duct and Islet Cells", Diabetes, American Diabetes Association, US, US , (20030701), vol. 52, no. 7, doi:10.2337/diabetes.52.7.1732, ISSN 0012-1797, pages 1732 - 1737, XP002549995
OPPOSITION- RIVENBARK et al., "Epigenetic reprogramming of cancer cells via targeted DNA methylation", Epigenetics, (20120000), vol. 7, no. 4, doi:10.4161/epi.19507, pages 350 - 360, XP002756482
OPPOSITION- Tautvydas Karvelis et al., "crRNA and tracrRNA guide Cas9-mediated DNA interference in Streptococcus thermophilus", RNA Biology, (2013), vol. 10, doi:10.4161/rna.24203, XP055539891
OPPOSITION- CHYLINSKI et al., "The tracrRNA and Cas9 families of type II CRISPR-Cas immunity systems", RNA Biology, (20130000), vol. 10, no. 5, doi:10.4161/rna.24321, pages 726 - 737, XP055116068
OPPOSITION- CHYLINSKI et al., "The tracrRNA and Cas9 families of type II CRISPR-Cas immunity systems", RNA Biology, (20130405), vol. 10, no. 5, doi:10.4161/rna.24321, pages 726 - 737, XP055116068
OTHER- "CRISPR-associated endonuclease Csn1I family protein [Neisseria meningitidis 98008]", Protein, (20120827), Database accession no. EJU65122.1, URL: ncbi, XP055598836
OTHER- "CRISPR-associated protein Cas9/Csn1 [Staphylococcus aureus subsp. aureus]", Protein, (20130103), Database accession no. CCK74173, URL: ncbi, XP055337941
OTHER- "CRISPR-associated protein Cas9/Csn1, subtype 11/NMEMI [Treponema sp. JC4]", Protein, (20120410), Database accession no. EID84762.1, URL: ncbi, XP055598819
OTHER- "CRISPR-associated protein, Csn1 family [Campylobacter jejuni subsp. jejuni 260.94]", Protein, (20060127), Database accession no. EAQ58399.1, URL: ncbi, XP055598855
OTHER- "Csn1 family CRISPR-associated protein [Mycoplasma canis UFG4]", Protein, (20120417), Database accession no. EIE41734.1, URL: ncbi, XP055598828
OTHER- "hypothetical protein W5M_11353 [Corynebacterium diphtheriae bv. intermedius str. NCTC 5011]", Protein, (20120521), Database accession no. EIK55275.1, URL: ncbi, XP055598805
OTHER- "Legionella pneumophila (strain Paris).", UniprotKB, (20041123), Database accession no. Q5X8T6, URL: Uniprot, XP055598811
OTHER- Zhijian Wu et al, "Effect of Genome Size on AAV Vector Packaging", MOLECULAR THERAPY, (20100101), vol. 18, no. 1, pages 80 - 86, XP055235504
OTHER- Händel E M et al, "Versatile and efficient genome editing in human cells by combining zinc-finger nucleases with adeno-associated viral vectors", HUMAN GENE THERAPY, (20111214), vol. 23, no. 3, pages 321 - 329, XP002730956
OTHER- L. CONG ET AL, "Multiplex Genome Engineering Using CRISPR/Cas Systems", SCIENCE, (20130215), vol. 339, no. 6121, pages 819 - 823, XP055102030
OTHER- Jensen Nanna M et al, "An update on targeted gene repair in mammalian cells: methods and mechanisms", Journal of Biomedical Science, (20110202), vol. 18, no. 1, page 10, XP021085735
SEARCH- ELITZA DELTCHEVA ET AL, "CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III", NATURE, (20110331), vol. 471, no. 7340, doi:10.1038/nature09886, ISSN 0028-0836, pages 602 - 607, XP055068535 [A] 1-28
SEARCH- BLAKE WIEDENHEFT ET AL, "RNA-guided genetic silencing systems in bacteria and archaea", NATURE, (20120215), vol. 482, no. 7385, doi:10.1038/nature10886, ISSN 0028-0836, pages 331 - 338, XP055116249 [A] 1-28 * the whole document *
SEARCH- JIANG WENYAN ET AL, "RNA-guided editing of bacterial genomes using CRISPR-Cas systems", NATURE BIOTECHNOLOGY,, (20130129), vol. 31, no. 3, pages 233 - 239, XP002699849 [PA] 1-28 * abstract; figures 1-5 *
SEARCH- G. GASIUNAS ET AL, "PNAS Plus: Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, (20120925), vol. 109, no. 39, doi:10.1073/pnas.1208507109, ISSN 0027-8424, pages E2579 - E2586, XP055068588 [X] 1,3-11,15-19 * abstract; figures 4, S9 * * page E2579 * * page E2583 * [I] 2,12-14,20-28
SEARCH- M. JINEK ET AL, "A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity", SCIENCE, (20120817), vol. 337, no. 6096, doi:10.1126/science.1225829, ISSN 0036-8075, pages 816 - 821, XP055067740 [X] 1,3-11,15-19 * the whole document * [I] 2,12-14,20-28

Download Citation Report

Get a free citation report including examiner, opposition, and international search citations.

Get Citation Report

Dossier Documents

The dossier documents provide a comprehensive record of the patent’s prosecution history - including filings, correspondence, and decisions made by patent offices - and are crucial for understanding the patent’s legal journey and any challenges it may have faced during examination.

  • Date

    Description

  • Get instant alerts for new documents