IP Verse

Crispr-Based Genome Modification And Regulation - EP3138912

The patent EP3138912 was granted to Sigma Aldrich on Dec 5, 2018. The application was originally filed on Dec 5, 2013 under application number EP16183725A. The patent is currently recorded with a legal status of "Revoked".

EP3138912

SIGMA ALDRICH
Application Number
EP16183725A
Filing Date
Dec 5, 2013
Status
Revoked
Aug 26, 2022
Publication Date
Dec 5, 2018
External Links
Slate, Register, Google Patents

Patent Summary

Patent Family

Patent Family

Patent Oppositions (6)

Patent oppositions filed by competitors challenge the validity of a granted patent. These oppositions are typically based on claims of prior art, lack of novelty, or non-obviousness. They are a key part of the process for determining a patent's strength and enforceability.

CompanyOpposition DateRepresentativeOpposition Status

Get instant alerts for new oppositions and patent status changes

POHLMANSep 5, 2019POHLMANADMISSIBLE
BASFSep 4, 2019EISENFUHR SPEISERADMISSIBLE
BAYER BAYERJun 18, 2019COHAUSZ & FLORACKADMISSIBLE
VOSSIUS & PARTNER PATENTANWALTE RECHTSANWALTE MBBFeb 22, 2019VOSSIUS & PARTNER PATENTANWALTE RECHTSANWALTE MBBADMISSIBLE
GRUNDJan 30, 2019GRUNDADMISSIBLE
SCHLICHDec 6, 2018SCHLICHADMISSIBLE

Patent Citations (10) New

Patent citations refer to prior patents cited during different phases such as opposition or international search.

Citation PhasePublication Number
OPPOSITIONWO2011146121
OPPOSITIONWO2012087756
OPPOSITIONWO2013176772
OPPOSITIONWO2014089290
OTHEREP3138910
OTHERWO2011146121
OTHERWO2013176772
SEARCHWO2013176772
SEARCHWO2014093655
SEARCHWO2014099744

Non-Patent Literature (NPL) Citations (168) New

NPL citations refer to non-patent references such as research papers, articles, or other publications cited during examination or opposition phases.

Citation PhaseReference Text
DESCRIPTION- BEERLI ET AL., NAT. BIOTECHNOL., (2002), vol. 20, pages 135 - 141
DESCRIPTION- BELFORT ET AL., NUCLEIC ACIDS RES., (1997), vol. 25, pages 3379 - 3388
DESCRIPTION- CHOO ET AL., CURR. OPIN. STRUCT. BIOL, (2000), vol. 10, pages 411 - 416
DESCRIPTION- CONG ET AL., SCIENCE, vol. 339, pages 819 - 823
DESCRIPTION- DAYHOFF, Atlas of Protein Sequences and Structure, NATIONAL BIOMEDICAL RESEARCH FOUNDATION, vol. 3, pages 353 - 358
DESCRIPTION- DOYON ET AL., NAT. BIOTECHNOL., (2008), vol. 26, pages 702 - 708
DESCRIPTION- DOYON ET AL., NAT. METHODS, (2011), vol. 8, pages 74 - 81
DESCRIPTION- GRIBSKOV, NUCL. ACIDS RES, (1986), vol. 14, no. 6, pages 6745 - 6763
DESCRIPTION- ISALAN ET AL., NAT. BIOTECHNOL., (2001), vol. 19, pages 656 - 660
DESCRIPTION- ISELI ET AL., PLOS ONE, vol. 2, no. 6, page E579
DESCRIPTION- JINEK ET AL., SCIENCE, vol. 337, pages 816 - 821
DESCRIPTION- LANGE ET AL., J. BIOL. CHEM., (2007), vol. 282, pages 5101 - 5105
DESCRIPTION- LOMBARDO; 2007 ET AL., NAT. BIOTECHNOLOGY, vol. 25, pages 1298 - 1306
DESCRIPTION- MILLER ET AL., NAT. BIOTECHNOL, (2007), vol. 25, pages 778 - 785
DESCRIPTION- MOEHLE ET AL., PNAS, (2007), vol. 104, pages 3055 - 3060
DESCRIPTION- PABO ET AL., ANN. REV. BIOCHEM., (2001), vol. 70, pages 313 - 340
DESCRIPTION- SANTIAGO ET AL., PNAS, (2008), vol. 105, pages 5809 - 5814
DESCRIPTION- SANTIAGO ET AL., PROC. NATL. ACAD. SCI. USA, (2008), vol. 105, pages 5809 - 5814
DESCRIPTION- SEGAL ET AL., CURR. OPIN. BIOTECHNOL., (2001), vol. 12, pages 632 - 637
DESCRIPTION- SMITH; WATERMAN, ADVANCES IN APPLIED MATHEMATICS, (1981), vol. 2, pages 482 - 489
DESCRIPTION- SZCZPEK ET AL., NAT. BIOTECHNOL, (2007), vol. 25, pages 786 - 793
DESCRIPTION- URNOV ET AL., NATURE, (2005), vol. 435, pages 646 - 651
DESCRIPTION- ZHANG ET AL., J. BIOL. CHEM., (2000), vol. 275, no. 43, pages 33850 - 33860
EXAMINATION- E. Kim ET AL, "Precision genome engineering with programmable DNA-nicking enzymes", Genome Research, (20120420), vol. 22, no. 7, doi:10.1101/gr.138792.112, ISSN 1088-9051, pages 1327 - 1333, XP055106309
OPPOSITION- "cNLS Mapper results; NLS sequence mapper", cNLS Mapper, URL: nls-mapper.iab.keio.ac.jp/cgi-bin/NLS_Mapper_yogi
OPPOSITION- CONG et al., "Multiplex Genome Engineering Using CRISPR/Cas Systems", Science, (20130000), vol. 339, no. 6121, pages 819 - 823, XP002779503
OPPOSITION- DAVIS, G.D. et al., "Zinc Finger Nucleases for Genome Editing", Genetic Engineering & Biotechnology News, (20100000), vol. 30, no. 13, XP055340181
OPPOSITION- "ENABLING SCIENCE TO IMPROVE THE QUALITY OF LIFE", Sigma-Aldrich Annual Report, (20120000), pages 1 - 92, XP055638459
OPPOSITION- FIECK et al., "Modifications of the E. coli Lac repressor for expression in eukaryotic cells: effects of nuclear signal sequences on protein activity and nuclear accumulation", Nucleic Acids Res., (19920000), vol. 20, no. 7, pages 1785 - 1791, XP002095852
OPPOSITION- FREITAS et al., "Mechanisms and Signals for the Nuclear Import of Proteins", Current Genomics, (20090000), vol. 10, pages 550 - 557, XP055502464
OPPOSITION- "in vivo", ALBERTS B. et al., Lehrbuch der Molekularen Zellbiologie, Weinheim, Wiley-VCH, (19990000), page 690, XP055844645
OPPOSITION- "in vivo", Gunter Mayer, Thieme Römpp, Thieme, (20090400), XP055844641
OPPOSITION- "Kallus", Dieter Heß, Pflanzenphysiologie, Stuttgart, Eugen Ulmer KG, (20080000), pages 62 - 63, XP055844643
OPPOSITION- SCHRAMM et al., "Recruitment of RNA polymerase III to its target promoters", Genes Dev, (20020000), vol. 16, pages 2593 - 2620, XP055183585
OPPOSITION- CRISTEA et al., "In vivo cleavage of transgene donors promotes nuclease-mediated targeted integration", Biotechnology and Bioengineering, (20130000), vol. 110, pages 871 - 880, XP055076901
OPPOSITION- SOUILHOL et al., "Nas transgenic mouse line allows visualization of Notch pathway activity in vivo", Genesis, (20060000), vol. 44, pages 277 - 286, XP055502571
OPPOSITION- FURUTAMA et al., "Expression of the IP3R1 promoter-driven nls-lacZ transgene in Purkinje cell parasagittal arrays of developing mouse cerebellum", J Neurosci Res, (20100000), vol. 88, pages 2810 - 2825, XP055502574
OPPOSITION- BIZZARRI et al., "Fluorescence recovery after photobleaching reveals the biochemistry of nucleocytoplasmic exchange", Anal Bioanal Chem, (20120000), vol. 403, pages 2339 - 2351, XP035069015
OPPOSITION- REMY, "Zinc-finger nucleases: a powerful tool for genetic engineering of animals", Transgenic Res, (20100000), vol. 19, pages 363 - 371, XP019831645
OPPOSITION- FOECKING et al., "Powerful and versatile enhancer-promoter unit for mammalian expression vectors", Gene, (19860000), pages 101 - 105, XP009061501
OPPOSITION- MARFORI et al., "Molecular basis for specificity of nuclear import and prediction of nuclear localization", Biochim. Biophys. Acta, (20110000), vol. 1813, doi:doi:10.1016/j.bbamcr.2010.10.013, pages 1562 - 1577, XP055378211
OPPOSITION- PEREZ-PINERA et al., "Advances in Targeted Genome Editing", NIH Public Access Author Manuscript, (20120000), pages 1 - 18, XP055501592
OPPOSITION- PEREZ-PINERA, P., "Advances in Targeted Genome Editing", Current Opinion in Chemical Biology, (20120000), vol. 16, no. 3-4, doi:doi:10.1016/j.cbpa.2012.06.007, pages 268 - 277, XP055065262
OPPOSITION- WANG et al., "One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering", Cell, (20130000), vol. 153, pages 910 - 918, XP028538358
OPPOSITION- RAN et al., "Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity", Cell, (20130000), vol. 154, pages 1380 - 1389, XP002718603
OPPOSITION- MUSSOLINO, C. et al., "TALE nucleases: tailored genome engineering made easy", Current Opinion Biotechnology, (20120000), vol. 23, no. 5, doi:doi:10.1016/j.copbio.2012.01.013, pages 644 - 650, XP055071668
OPPOSITION- MUSSOLINO, "TALE nucleases: tailored genome engineering made easy", Curr Opinion Biotechnol, (20120000), vol. 23, pages 644 - 650, XP055071668
OPPOSITION- DO et al., "Identification of multiple nuclear localization signals in murine Elf3, an ETS transcription factor", FEBS Letters, (20060000), vol. 580, no. 7, doi:doi:10.1016/j.febslet.2006.02.049, pages 1865 - 71, XP028030315
OPPOSITION- GOLDFARB et al., "Importin @a: a multipurpose nuclear-transport receptor", Trends in Cell Biology, (20040000), vol. 14, pages 505 - 514, XP004552656
OPPOSITION- WARD et al., "Targeted integration of a rAAV vector into the AAVS1 region", Virology, (20120000), vol. 433, pages 356 - 366, XP055502331
OPPOSITION- KARRAN, "DNA double strand break repair in mammalian cells", Current Opinion in Genetics & Development, (20000000), vol. 10, pages 144 - 150, XP001206008
OPPOSITION- CHANG et al., "Genome editing with RNA-guided Cas9 nuclease in zebrafish embryos", Cell Research, (20130000), vol. 23, no. 4, doi:doi:10.1038/cr.2013.45, page 465, XP055251146
OPPOSITION- CHANG, "Genome editing with RNA-guided Cas9 nuclease in Zebrafish embryos", Cell Research, (20130000), vol. 23, pages 465 - 472, XP055251146
OPPOSITION- CARROLL, D., "Progress and prospects: zinc-finger nucleases as gene therapy agents", Gene Therapy, (20080000), vol. 15, doi:doi:10.1038/gt.2008.145, pages 1463 - 1468, XP055041104
OPPOSITION- D CARROLL, "Progress and prospects: Zinc-finger nucleases as gene therapy agents", Gene Therapy, (20080000), vol. 15, no. 22, pages 1463 - 1468, XP055041104
OPPOSITION- LEI et al., "Gene Editing of Human Embryonic Stem Cells via an Engineered Baculoviral Vector Carrying Zinc-finger Nucleases", Molecular Therapy, (20110000), vol. 19, pages 942 - 950, XP055639632
OPPOSITION- CARROLL, D., "A CRISPR Approach to Gene Targeting", Molecular Therapy, (20120000), vol. 20, no. 9, doi:doi:10.1038/mt.2012.171, pages 1658 - 1660, XP055106489
OPPOSITION- CARROLL, D., "A CRISPR Approach to Gene Targeting", Mol. Therap., (20120000), vol. 20, no. 9, doi:doi:10.1038/mt.2012.171, pages 1658 - 1660, XP055106489
OPPOSITION- CARLSON et al., "Targeting DNA With Fingers and TALENs", Molecular Therapy-Nucleic Acids, (20120100), vol. 1, no. 1, pages 1 - 4, XP055182775
OPPOSITION- URNOV, F.D. et al., "Highly efficient endogenous human gene correction using zinc-finger nucleases", Nature, (20050000), vol. 435, doi:doi:10.1038/nature03556, pages 646 - 651, XP002411069
OPPOSITION- LI et al., "In vivo genome editing restores haemostasis in a mouse model of haemophilia", HHS Public Access Author Manuscript, (20110000), vol. 475, doi::10.1038/nature10177, pages 1 - 20, XP055502541
OPPOSITION- BEDELL et al., "In vivo genome editing using a high-efficiency TALEN system", Nature, (20120000), vol. 491, pages 114 - 118, XP055048281
OPPOSITION- BEDELL, V.M. et al., "In vivo genome editing using a high-efficiency TALEN system", Nature, (20120000), vol. 491, doi:doi:10.1038/nature11537, pages 114 - 118, XP055048281
OPPOSITION- PORTEUS, M.H. et al., "Gene targeting using zinc finger nucleases", Nature Biotechnology, (20050000), vol. 23, no. 8, doi:doi:10.1038/nbt1125, pages 967 - 973, XP002467422
OPPOSITION- LOMBARDO et al., "TI - Gene editing in human stem cells using zinc finger nucleases and integrase-defective lentiviral vector delivery", Nature Biotechnology, (20070000), vol. 11, pages 1298 - 1306, XP002633002
OPPOSITION- IRION et al., "Identification and targeting of the ROSA26 locus in human embryonic stem cells", Nature Biotechnology, (20070000), vol. 25, pages 1477 - 1482, XP008111005
OPPOSITION- HOCKEMEYER et al., "Highly efficient gene targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases", Nat. Biotechnol., (20090000), vol. 27, page 851, XP055390559
OPPOSITION- CUI et al., "Targeted integration in rat and mouse embryos with zinc-finger nucleases", Nat Biotechnol, (20110000), vol. 29, pages 64 - 67, XP055065200
OPPOSITION- MILLER, "A TALE nuclease architecture for efficient genome editing.(includes Online Methods)", Nature Biotechnology, (20110000), vol. 29, pages 143 - 148, XP002685898
OPPOSITION- MILLER, J.C. et al., "A TALE nuclease architecture for efficient genome editing", Nature Biotechnology, (20110000), vol. 29, no. 2, doi:doi:10.1038/nbt.1755, pages 143 - 148, XP055568321
OPPOSITION- HOCKEMEYER et al., "Genetic engineering of human pluripotent cells using TALE nucleases", Nature Biotechnology, (20110000), vol. 29, pages 731 - 734, XP055018244
OPPOSITION- TESSON et al., "Knockout rats generated by embryo microinjection of TALENs", Nat Biotechnol, (20110000), vol. 29, pages 695 - 696, XP055145023
OPPOSITION- REYON, D. et al., "FLASH Assembly of TALENS Enables High-Throughput Genome Editing", Nature Biotechnology, (20120000), vol. 30, no. 5, pages 460 - 465, XP055565802
OPPOSITION- BARRANGOU, "RNA-mediated programmable DNA cleavage", Nature Biotechnology, (20120000), vol. 30, no. 9, doi:doi:10.1038/nbt.2357, pages 836 - 838, XP055118675
OPPOSITION- BARRANGOU, "RNA-mediated programmable DNA cleavage", Nature Biotechnology, (20120000), vol. 30, no. 9, pages 836 - 838, XP055118675
OPPOSITION- HWANG et al., "Efficient genome editing in zebrafish using a CRISPR-Cas system", Nature Biotechnology, (20130000), vol. 31, pages 227 - 229, XP055086625
OPPOSITION- HWANG, W. et al., "Efficient genome editing in zebrafish using a CRISPR-Cas system", Nature Biotechnology, (20130000), vol. 31, doi:doi:10.1038/nbt.2501, pages 227 - 229, XP055540926
OPPOSITION- CHO et al., "Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease (includes Supplementary information)", Nature Biotechnology, (20130000), vol. 31, pages 230 - 232,1-11, XP002699850
OPPOSITION- MALI et al., "CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering", HHS Public Access Author Manuscript, (20130000), vol. 31, pages 1 - 17, XP055541952
OPPOSITION- OAKES et al., "Profiling of engineering hotspots identifies an allosteric CRISPR- Cas9 switch", Nature Biotech, (20160000), pages 1 - 14, XP055378225
OPPOSITION- REBAR et al., "Induction of angiogenesis in a mouse model using engineered transcription factors", Nature Medicine, (20020000), vol. 8, pages 1427 - 1432, XP002993087
OPPOSITION- SADELAIN et al., "S afe harbours for the integration of new DNA in the human genome", Nat. Rev. Cancer, (20120000), vol. 12, no. 1, doi:doi:10.1038/nrc3179, pages 51 - 58, XP055018235
OPPOSITION- URNOV et al., "Genome editing with engineered zinc finger nucleases", Nat Rev. Genet, (20100911), vol. 11, doi:doi:10.1038/nrg2842, pages 636 - 646, XP055198280
OPPOSITION- URNOV, F.D. et al., "Genome editing with engineered zinc finger nucleases", Nature Reviews Genetics, (20100000), vol. 11, doi:doi:10.1038/nrg2842, pages 636 - 646, XP055198280
OPPOSITION- URNOV, "Genome editing with engineered zinc finger nucleases", Nat. Rev. Gene, (20100000), vol. 11, pages 636 - 646, XP055198280
OPPOSITION- JOUNG et al., "TALENs: a widely applicable technology for targeted genome editing", Nat. Rev. Mol. Cell Biol., (20130000), vol. 14, no. 1, doi:doi:10.1038/nrm3486, pages 49 - 55, XP055282847
OPPOSITION- JOUNG et al., "TALENs: a widely applicable technology for targeted genome editing", Nature Reviews, (20130000), vol. 14, pages 49 - 55, XP055282847
OPPOSITION- LAUMONIER et al., "Lentivirus mediated HO-1 gene transfer enhances myogenic precursor cell survival after autologous transplantation in pig", Mol Ther, (20080000), vol. 16, pages 404 - 410, XP008159717
OPPOSITION- MOEHLE et al., "Targeted gene addition into a specified location in the human genome using designed zinc finger nucleases + corrections", PNAS, (20070403), vol. 104, no. 14, pages 3055 - 3060, 6090, XP002518477
OPPOSITION- BEUMER et al., "Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases", PNAS, (20080000), vol. 105, pages 19821 - 19826, XP002782686
OPPOSITION- MAHFOUZ, M.M. et al., "De novo-engineered transcription activator-like effector (TALE) hybrid nuclease with novel DNA binding specificity creates double- strand breaks", PNAS, (20110000), vol. 108, no. 6, doi:doi:10.1073/pnas.1019533108, pages 2623 - 2628, XP055007615
OPPOSITION- GASIUNAS et al., "Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria", PNAS, (20120925), vol. 109, no. 39, pages e2579 - e2586, XP055068588
OPPOSITION- LIANG, "Homology-directed repair is a major double-strand break repair pathway in mammalian cells", PNAS, (19980400), vol. 95, pages 5172 - 5177, XP055502633
OPPOSITION- DAI et al., "The transcription factors GATA4 and dHAND physically interact to synergistically activate cardiac gene expression through a p300-dependent mechanism", J. Biol. Chem., (20020000), vol. 277, no. 27, doi:doi:10.1074/jbc.M202490200, pages 24390 - 8, XP055251716
OPPOSITION- PLANEY et al., "Inhibition of glucocorticoid-induced apoptosis in 697 pre-B lymphocytes by the mineralocorticoid receptor N-terminal domain", J. Biol. Chem., (20020000), vol. 277, no. 44, doi:doi:10.1074/jbc.M205085200, pages 42188 - 96, XP055251719
OPPOSITION- HODEL et al., "Nuclear Localization Signal Receptor Affinity Correlates with in Vivo Localization in Saccharomyces cerevisiae", JBC, (20060000), vol. 281, no. 33, pages 23545 - 23556, XP055502581
OPPOSITION- HANDEL, E.M. et al., "Versatile and Efficient Genome Editing in Human Cells by Combining Zinc-Finger Nucleases With Adeno-Associated Viral Vectors", Human Gene Therapy, (20120000), vol. 23, doi:doi:10.1089/hum.2011.140, pages 321 - 329, XP002730956
OPPOSITION- HÄNDEL et al., "Versatile and efficient genome editing in human cells by combining zinc-finger nucleases with adeno-associated viral vectors", Human Gene Therapy, (20120000), vol. 23, doi:doi:10.1089/hum.2011.140, pages 321 - 329, XP002730956
OPPOSITION- KOURANOVA et al., "CRISPRs for Optimal Targeting: Delivery of CRISPR Components as DNA, RNA, and Protein into Cultured Cells and Single- Cell Embryos", Hum. Gene . Ther., (20160000), vol. 27, no. 6, doi:doi:10.1089/hum.2016.009, pages 464 - 75, XP055465083
OPPOSITION- SALOMON et al., "Capture of genomic and T-DNA sequences during double-strand break repair in somatic plant cells", The EMBO Journal, (19980000), vol. 17, pages 6086 - 6095, XP002259898
OPPOSITION- PUCHTA, "The repair of double-strand breaks in plants: mechanisms and consequences for genome evolution", Journal of Experimental Botany, (20050000), vol. 56, pages 1 - 14, XP002394840
OPPOSITION- ORLANDO et al., "Zinc-finger nuclease-driven targeted integration into mammalian genomes using donors with limited chromosomal homology", Nucleic Acid Res, (20100000), vol. 38, pages 1 - 15, XP055076783
OPPOSITION- LI et al., "Modularly assembled designer TAL effector nucleases for targeted gene knockout and gene replacement in eukaryotes", Nucleic Acids Res., (20110000), vol. 39, no. 14, doi:doi:10.1093/NAR/GKR188, pages 6315 - 25, XP002681969
OPPOSITION- LI, T. et al., "Modularly assembled designer TAL effector nucleases for targeted gene knockout and gene replacement in eukaryotes", Nucleic Acids Research, (20110000), vol. 39, no. 14, doi:doi:10.1093/NAR/GKR188, pages 6315 - 6325, XP002681969
OPPOSITION- CERMAK et al., "Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting", Nucleic Acids Res, (20110000), vol. 39, pages 1 - 11, XP055130093
OPPOSITION- CERMAK, T. et al., "Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting", Nucleic Acids Research, (20110000), vol. 39, no. 12, doi:doi:10.1093/nar/gkr218, page e82, XP055130093
OPPOSITION- RAMACHANDRA et al., "Efficient recombinase-mediated cassette exchange at the AAVS1 locus in human embryonic stem cells using baculoviral vector s", Nucleic Acids Res., (20110000), vol. 39, no. 16, doi:doi:10.1093/nar/gkr409, page e107, XP055264872
OPPOSITION- DICARLO et al., "Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems", Nucleic Acids Res, (20130000), vol. 41, pages 4336 - 4343, XP055086617
OPPOSITION- DICARLO et al., "Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems", Nucleic Acids Res., (20130304), vol. 41, no. 7, pages 4336 - 4343, XP055086617
OPPOSITION- KIM et al., "Precision genome engineering with programmable DNA-nicking enzymes", Genome Research, (20120000), vol. 22, no. 7, pages 1327 - 1333, XP055106309
OPPOSITION- MARESCA et al., "Obligate Ligation-Gated Recombination (ObLiGaRe): Custom-designed nuclease-mediated targeted integration through nonhomologous end joining", Genome Research, (20130000), vol. 23, pages 539 - 546, XP055077484
OPPOSITION- CHO et al., "Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases", Genome Research, (20140000), vol. 24, pages 132 - 141, XP055227885
OPPOSITION- SHIEH et al., "Nuclear Targeting of the maize R protein requires two nuclear localization sequences", Plant Physiol., (19930000), vol. 101, no. 2, doi:doi:10.1104/pp.101.2.353, pages 353 - 361, XP055241771
OPPOSITION- LUO et al., "Multiple nuclear localization sequences allow modulation of 5- lipoxygenase nuclear impor t", Traffic, (20040000), vol. 5, no. 11, doi:doi:10.1111/j.1600-0854.2004.00227.x, pages 847 - 54, XP055267791
OPPOSITION- PORTEUS et al., "Chimeric nucleases stimulate gene targeting in human cells", Science, (20030000), vol. 300, page 763, XP002974231
OPPOSITION- BIBIKOVA et al., "Enhancing gene targeting with designed zinc finger nucleases", Science, (20030000), vol. 300, doi:10.1126/science.1079512, page 764, XP002974267
OPPOSITION- GEURTS et al., "Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases", Science, (20090000), vol. 325, page 433, XP002580718
OPPOSITION- JINEK et al., "A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity", Science, (20120000), vol. 337, pages 816 - 820, XP055067740
OPPOSITION- JINEK et al., "A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity", Science, (20120817), vol. 337, no. 6096, doi:doi:10.1126/science.1225829, pages 816 - 821, XP055549487
OPPOSITION- JINEK, M. et al., "A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity", Science, (20120000), vol. 337, doi:doi:10.1126/science.1225829, pages 816 - 821, XP055549487
OPPOSITION- BROUNS, "A Swiss Army Knife of Immunity", Science, (20120000), vol. 337, pages 808 - 809, XP055113718
OPPOSITION- BROUNS, "A Swiss Army Knife of Immunity", Science, (20120628), vol. 337, no. 6096, doi:doi:10.1126/science.1227253, pages 808 - 809, XP055113718
OPPOSITION- BROUNS, S.J.J., "A Swiss Army Knife of Immunity", Science, (20120000), vol. 337, doi:doi:10.1126/science.1227253, pages 808 - 809, XP055113718
OPPOSITION- CONG et al., "Multiplex genome engineering using CRISPR/Cas systems", Science, (20130000), vol. 339, doi:doi:10.1126/science.1231143, pages 819 - 823, XP055400719
OPPOSITION- CONG, L. et al., "Multiplex Genome Engineering Using CRISPR/Cas Systems", Science, (20130000), vol. 339, doi:doi:10.1126/science.1231143, pages 819 - 823, XP055400719
OPPOSITION- MALI et al., "RNA-Guided Human Genome Engineering via Cas9", Science, (20130000), vol. 339, no. 6121, pages 823 - 826, XP055111247
OPPOSITION- MALI et al., "RNA-guided human genome engineering via Cas9", Science, (20130103), vol. 339, no. 6121, pages 823 - 826, XP055111247
OPPOSITION- MALI, P. et al., "RNA-guided human genome engineering via Cas9", Science, (20130000), vol. 339, pages 823 - 826, XP055111247
OPPOSITION- ROUET et al., "Introduction of double-strand breaks into the genome of mouse cells by expression of a rare-cutting endonuclease", Molecular and Cellular Biology, (19940000), vol. 14, pages 8096 - 8106, XP000572151
OPPOSITION- L. FISCHER-FANTUZZI et al., "Cell -dependent efficiency of reiterated nuclear signals in a mutant simian virus 40 oncoprotein targeted to the nucleus", Mol Cell Biol., (19880000), vol. 8, no. 12, doi:doi:10.1128/MCB.8.12.549, pages 5495 - 5503, XP009140368
OPPOSITION- RUDIN et al., "Efficient repair of HO-induced chromosomal breaks in Saccharomyces cerevisiae by recombination between flanking homologous sequences", Molecular and Cellular Biology, (19880000), vol. 8, pages 3918 - 3928, XP055502440
OPPOSITION- Michael R LIEBER, "The Mechanism of Double-Strand DNA Break Repair by the Nonhomologous DNA End-Joining Pathway", NIH Public Access Author Manuscript, (20100000), vol. 79, no. 1, pages 181 - 211, XP055502406
OPPOSITION- MINAMI et al., "Ets Motifs Are Necessary for Endothelial Cell -Specific Expression of a 723-bp Tie-2 Promoter/Enhancer in Hprt Targeted Transgenic Mice", Arterioscler Thromb Vasc Biol., (20030000), vol. 23, pages 2041 - 2047, XP055502336
OPPOSITION- MCCONNELL et al., "Nuclear and cytoplasmic LIMK1 enhances human breast cancer progression", Molecular Cancer, (20110000), vol. 10, pages 1 - 13, XP021103688
OPPOSITION- ZHANG et al., "Applications of mRNA injections for analyzing cell lineage and asymmetric cell divisions during segmentation in the leech Helobdella robusta", Development, (20050000), vol. 132, no. 9, pages 2103 - 2113, XP055502340
OPPOSITION- CHANG, "Microtubule-based localization of a synaptic calcium-signaling complex is required for left-right neuronal asymmetry in C. elegans", Development, (20110000), vol. 138, pages 3509 - 3418, XP055502632
OPPOSITION- RAYMOND et al., "High-Efficiency FLP and PHIC31 Site-Specific Recombination in Mammalian Cells", PLoS ONE, (20070000), vol. 2, no. 1, doi:doi:10.1371/journal.pone.0000162, XP055069825
OPPOSITION- CAMPEAU et al., "A versatile viral system for expression and depletion of proteins in mammalian cells", PLoS One, (20090806), vol. 4, no. 8, doi:doi:10.1371/journal.pone.0006529, page e6529, XP055251120
OPPOSITION- HU et al., "Comparison of Various Nuclear Localization Signal-Fused Cas9 Proteins and Cas9 mRNA for Genome Editing in Zebrafish", G3, (20180000), vol. 8, doi:doi:10.1534/g3.117.300359, pages 823 - 831, XP055513510
OPPOSITION- CHRISTIAN, M. et al., "Targeting DNA Double-Strand Breaks with TAL Effector Nucleases", Genetics, (20100000), vol. 186, pages 757 - 761, XP002632806
OPPOSITION- CHRISTIAN M. et al., "Targeting DNA Double-Strand Breaks with TAL Effector Nucleases", Genetics, (20100726), vol. 186, no. 2, pages 757 - 761, XP002632806
OPPOSITION- CARROLL, D., "Genome Engineering with Zinc-Finger Nucleases", Genetics, (20110000), vol. 188, doi:doi:10.1534/genetics.111.131433, pages 773 - 782, XP055171682
OPPOSITION- CARROLL, "Genome Engineering With Zinc-Finger Nucleases", Genetics, (20110000), vol. 188, pages 773 - 782, XP055036700
OPPOSITION- GRATZ et al., "Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease", Genetics, (20130000), vol. 194, doi:10.1534/genetics.113.152710, pages 1029 - 1035, XP055102690
OPPOSITION- GOLIC et al., "RNA-Guided Nucleases: A New Era for Engineering the Genomes of Model and Nonmodel Organisms", Genetics, (20130000), vol. 195, pages 303 - 308, XP055340188
OPPOSITION- LYSSENKO, "Cognate putative nuclear localization signal effects strong nuclear localization of a GFP reporter and facilitates gene expression studies in Caenorhabditis elegans", BioTechniques, (20070000), vol. 43, pages 596 - 600, XP055268137
OPPOSITION- JINEK, "RNA-programmed genome editing in human cells", eLife, (20130000), vol. 2, pages 1 - 9, XP002699851
OTHER- Pablo Perez-Pinera, David G. Ousterout, and Charles A. Gersbach, "Advances in Targeted Genome Editing", NIH Public Access Author Manuscript, (20120000), pages 1 - 17, XP055447240
OTHER- Bassett Andrew R; Liu Ji-Long, "CRISPR/Cas9 and Genome Editing inDrosophila", J Genet Genomics, (20140000), vol. 41, no. 1, pages 7 - 19, XP028826531
OTHER- CHIU W-L; ET AL, "ENGINEERED GFP AS A VITAL REPORTER IN PLANTS", Curr Biol, (19960301), vol. 6, no. 3, pages 325 - 330, XP000571865
OTHER- BAKER M; ET AL, "GENE EDITING AT CRISPR SPEED", Nat Biotechnol., (20140400), vol. 32, no. 4, pages 309 - 312, XP003035060
OTHER- Christopher S Raymond; Philippe Soriano, "High-Efficiency FLP and ΦC31 Site-Specific Recombination in Mammalian Cells", PLoS One., (20070100), vol. 2, no. 1, pages e162/1 - e162/4, XP055069825
SEARCH- RODOLPHE BARRANGOU, "CRISPR-Cas systems and RNA-guided interference", WILEY INTERDISCIPLINARY REVIEWS: RNA, (20130320), vol. 4, no. 3, doi:10.1002/wrna.1159, ISSN 1757-7004, pages 267 - 278, XP055153534 [AP] 1-50
SEARCH- F. ANN RAN ET AL, "Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity", CELL, US, (20130901), vol. 154, no. 6, doi:10.1016/j.cell.2013.08.021, ISSN 0092-8674, pages 1380 - 1389, XP055299681 [AP] 1-50
SEARCH- EMMANUELLE CHARPENTIER ET AL, "Biotechnology: Rewriting a genome", NATURE, United Kingdom, (20130306), vol. 495, no. 7439, doi:10.1038/495050a, ISSN 0028-0836, pages 50 - 51, XP055338357 [AP] 1-50
SEARCH- BIN SHEN ET AL, "Generation of gene-modified mice via Cas9/RNA-mediated gene targeting", CELL RESEARCH - XIBAO YANJIU, GB, CN, (20130501), vol. 23, no. 5, doi:10.1038/cr.2013.46, ISSN 1001-0602, pages 720 - 723, XP055141533 [AP] 1-50
SEARCH- DANA CARROLL, "A CRISPR Approach to Gene Targeting", MOLECULAR THERAPY, (20120901), vol. 20, no. 9, doi:10.1038/mt.2012.171, ISSN 1525-0016, pages 1658 - 1660, XP055106489 [A] 1-50 * the whole document *
SEARCH- RODOLPHE BARRANGOU, "RNA-mediated programmable DNA cleavage", NATURE BIOTECHNOLOGY, (20120910), vol. 30, no. 9, doi:10.1038/nbt.2357, ISSN 1087-0156, pages 836 - 838, XP055118675 [A] 1-50
SEARCH- WOONG Y HWANG ET AL, "Efficient genome editing in zebrafish using a CRISPR-Cas system", NATURE BIOTECHNOLOGY, (20130129), vol. 31, no. 3, doi:10.1038/nbt.2501, ISSN 1087-0156, pages 227 - 229, XP055086625 [YP] 12,13,44 * the whole document *
SEARCH- PRASHANT MALI ET AL, "CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering", NATURE BIOTECHNOLOGY, US, (20130801), vol. 31, no. 9, doi:10.1038/nbt.2675, ISSN 1087-0156, pages 833 - 838, XP055299678 [AP] 1-50
SEARCH- G. GASIUNAS ET AL, "PNAS Plus: Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, (20120925), vol. 109, no. 39, doi:10.1073/pnas.1208507109, ISSN 0027-8424, pages E2579 - E2586, XP055068588 [A] 1-50
SEARCH- R. SAPRANAUSKAS ET AL, "The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli", NUCLEIC ACIDS RESEARCH, GB, (20110803), vol. 39, no. 21, doi:10.1093/nar/gkr606, ISSN 0305-1048, pages 9275 - 9282, XP055265024 [A] 1-50 * the whole document *
SEARCH- M. JINEK ET AL, "A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity", SCIENCE, US, (20120817), vol. 337, no. 6096, doi:10.1126/science.1225829, ISSN 0036-8075, pages 816 - 821, XP055229606 [A] 1-50
SEARCH[ ] - M. JINEK ET AL, "Supplementary Materials for A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity", SCIENCE, US, (20120628), vol. 337, no. 6096, doi:10.1126/science.1225829, ISSN 0036-8075, pages 1 - 37, XP055067747
SEARCH- L. CONG ET AL, "Multiplex Genome Engineering Using CRISPR/Cas Systems", SCIENCE, (20130214), vol. 339, no. 6121, doi:10.1126/science.1231143, ISSN 0036-8075, pages 819 - 823, XP055236048 [XP] 1-6,8-10,12-28,30-48,50 * the whole document * [Y] 7,12,13,29,44
SEARCH- MALI P. ET AL, "RNA-guided human genome engineering via Cas9", SCIENCE, (20130215), vol. 339, no. 6121, doi:10.1126/science.1232033, ISSN 0036-8075, pages 823 - 826, XP055300091 [YP] 7,29 * the whole document *
SEARCH- P. Mali ET AL, "Supplementary Materials for RNA-Guided Human Genome Engineering via Cas9", Science, (20130103), vol. 339, no. 6121, doi:10.1126/science.1232033, ISSN 0036-8075, pages 1 - 36, XP055322657
SEARCH- MARTIN JINEK ET AL, "RNA-programmed genome editing in human cells", ELIFE, GB, (20130129), vol. 2, doi:10.7554/eLife.00471, ISSN 2050-084X, pages e00471 - 1, XP055245475 [AP] 1-50 * the whole document *

Download Citation Report

Get a free citation report including examiner, opposition, and international search citations.

Get Citation Report

Dossier Documents

The dossier documents provide a comprehensive record of the patent’s prosecution history - including filings, correspondence, and decisions made by patent offices - and are crucial for understanding the patent’s legal journey and any challenges it may have faced during examination.

  • Date

    Description

  • Get instant alerts for new documents