Patent No. EP3930478 (titled "Stable Protein Formulations") was filed by Amano Enzyme on Feb 25, 2020. The application was issued on Jan 29, 2025.
Stable protein solutions for use in acidic beverages and beverage additives that contain a protein, stabilizer, and protein deamidating enzyme. The solutions have a pH of 3.5-7.0 and are stable against protein precipitation at acidic pH. The protein deamidating enzyme converts glutamine and asparagine residues in the protein to glutamic acid and aspartic acid, respectively, to increase the negative charge of the protein and lower its isoelectric point, making it more soluble at acidic pH. The stabilizer prevents sedimentation. The enzyme is added to the protein solution, incubated, and then acidified to the desired pH.

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