Composition For Cleaving A Target Dna Comprising A Guide Rna Specific For The Target Dna And Cas Protein-Encoding Nucleic Acid Or Cas Protein, And Use Thereof

A composition for cleaving target DNA in eukaryotic cells or organisms using a CRISPR/Cas9 system. The composition comprises a guide RNA specific for the target DNA and Cas9 protein or Cas9 protein-encoding nucleic acid. It enables precise genome editing in eukaryotes by introducing double-strand breaks at desired locations using the RNA-guided Cas9 endonuclease. The composition can be used for genotyping mutations and variations in eukaryotic cells and organisms.